Search Results - "TAKEDA, Junji"

Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon by Bradley, Allan, Yusa, Kosuke, Rad, Roland, Takeda, Junji

“…piggyBac transposons carrying reprogramming factors are used to reprogram mouse embryonic fibroblasts, with efficiencies equivalent to retroviral transduction,…”
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CRISPR-Cas3 induces broad and unidirectional genome editing in human cells by Morisaka, Hiroyuki, Yoshimi, Kazuto, Okuzaki, Yuya, Gee, Peter, Kunihiro, Yayoi, Sonpho, Ekasit, Xu, Huaigeng, Sasakawa, Noriko, Naito, Yuki, Nakada, Shinichiro, Yamamoto, Takashi, Sano, Shigetoshi, Hotta, Akitsu, Takeda, Junji, Mashimo, Tomoji

“…Although single-component Class 2 CRISPR systems, such as type II Cas9 or type V Cas12a (Cpf1), are widely used for genome editing in eukaryotic cells, the…”
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Mesenchymal to Epithelial Transition Induced by Reprogramming Factors Attenuates the Malignancy of Cancer Cells by Takaishi, Mikiro, Tarutani, Masahito, Takeda, Junji, Sano, Shigetoshi

“…Epithelial to mesenchymal transition (EMT) is a biological process of metastatic cancer. However, an effective anticancer therapy that directly targets the EMT…”
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CLICK: one-step generation of conditional knockout mice by Miyasaka, Yoshiki, Uno, Yoshihiro, Yoshimi, Kazuto, Kunihiro, Yayoi, Yoshimura, Takuji, Tanaka, Tomohiro, Ishikubo, Harumi, Hiraoka, Yuichi, Takemoto, Norihiko, Tanaka, Takao, Ooguchi, Yoshihiro, Skehel, Paul, Aida, Tomomi, Takeda, Junji, Mashimo, Tomoji

“…CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Here, we show that…”
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Generation of targeted homozygosity in the genome of human induced pluripotent stem cells by Yoshimura, Yasuhide, Yamanishi, Ayako, Kamitani, Tomo, Kim, Jin-Soo, Takeda, Junji

“…When loss of heterozygosity (LOH) is correlated with loss or gain of a disease phenotype, it is often necessary to identify which gene or genes are involved…”
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Chromatin states shape insertion profiles of the piggyBac, Tol2 and Sleeping Beauty transposons and murine leukemia virus by Yoshida, Junko, Akagi, Keiko, Misawa, Ryo, Kokubu, Chikara, Takeda, Junji, Horie, Kyoji

“…DNA transposons and retroviruses are versatile tools in functional genomics and gene therapy. To facilitate their application, we conducted a genome-wide…”
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PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia by Tamai, Katsuto, Yamazaki, Takehiko, Chino, Takenao, Ishii, Masaru, Otsuru, Satoru, Kikuchi, Yasushi, Iinuma, Shin, Saga, Kotaro, Nimura, Keisuke, Shimbo, Takashi, Umegaki, Noriko, Katayama, Ichiro, Miyazaki, Jun-ichi, Takeda, Junji, McGrath, John A., Uitto, Jouni, Kaneda, Yasufumi, Prockop, Darwin J.

“…The role of bone marrow cells in repairing ectodermal tissue, such as skin epidermis, is not clear. To explore this process further, this study examined a…”
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When Half Is Better Than the Whole: Advances in Haploid Embryonic Stem Cell Technology by Kokubu, Chikara, Takeda, Junji

“…Recent advances in embryonic stem cell (ESC) derivation and genome editing offer efficient platforms for genetic screening. In this issue, Li et al. and Leeb…”
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Author Correction: A newly identified gene Ahed plays essential roles in murine haematopoiesis by Nakai, Ritsuko, Yokota, Takafumi, Tokunaga, Masahiro, Takaishi, Mikiro, Yokomizo, Tomomasa, Sudo, Takao, Shi, Henyun, Yasumizu, Yoshiaki, Okuzaki, Daisuke, Kokubu, Chikara, Tanaka, Sachiyo, Takaoka, Katsuyoshi, Yamanishi, Ayako, Yoshida, Junko, Watanabe, Hitomi, Kondoh, Gen, Horie, Kyoji, Hosen, Naoki, Sano, Shigetoshi, Takeda, Junji

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A newly identified gene Ahed plays essential roles in murine haematopoiesis by Nakai, Ritsuko, Yokota, Takafumi, Tokunaga, Masahiro, Takaishi, Mikiro, Yokomizo, Tomomasa, Sudo, Takao, Shi, Henyun, Yasumizu, Yoshiaki, Okuzaki, Daisuke, Kokubu, Chikara, Tanaka, Sachiyo, Takaoka, Katsuyoshi, Yamanishi, Ayako, Yoshida, Junko, Watanabe, Hitomi, Kondoh, Gen, Horie, Kyoji, Hosen, Naoki, Sano, Shigetoshi, Takeda, Junji

“…The development of haematopoiesis involves the coordinated action of numerous genes, some of which are implicated in haematological malignancies. However, the…”
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Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro by Yagita, Kazuhiro, Horie, Kyoji, Koinuma, Satoshi, Nakamura, Wataru, Yamanaka, Iori, Urasaki, Akihiro, Shigeyoshi, Yasufumi, Kawakami, Koichi, Shimada, Shoichi, Takeda, Junji, Uchiyama, Yasuo

“…The molecular oscillations underlying the generation of circadian rhythmicity in mammals develop gradually during ontogenesis. However, the developmental…”
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Limb-clasping, cognitive deficit and increased vulnerability to kainic acid-induced seizures in neuronal glycosylphosphatidylinositol deficiency mouse models by Kandasamy, Lenin C, Tsukamoto, Mina, Banov, Vitaliy, Tsetsegee, Sambuu, Nagasawa, Yutaro, Kato, Mitsuhiro, Matsumoto, Naomichi, Takeda, Junji, Itohara, Shigeyoshi, Ogawa, Sonoko, Young, Larry J, Zhang, Qi

“…Abstract Posttranslational modification of a protein with glycosylphosphatidylinositol (GPI) is a conserved mechanism exists in all eukaryotes. Thus far, >150…”
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FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice by Misaka, Tomofumi, Murakawa, Tomokazu, Nishida, Kazuhiko, Omori, Yosuke, Taneike, Manabu, Omiya, Shigemiki, Molenaar, Chris, Uno, Yoshihiro, Yamaguchi, Osamu, Takeda, Junji, Shah, Ajay M., Otsu, Kinya

“…Protein quality control in cardiomyocytes is crucial to maintain cellular homeostasis. The accumulation of damaged organelles, such as mitochondria and…”
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Elimination of protein aggregates prevents premature senescence in human trisomy 21 fibroblasts by Nawa, Nobutoshi, Hirata, Katsuya, Kawatani, Keiji, Nambara, Toshihiko, Omori, Sayaka, Banno, Kimihiko, Kokubu, Chikara, Takeda, Junji, Nishimura, Ken, Ohtaka, Manami, Nakanishi, Mahito, Okuzaki, Daisuke, Taniguchi, Hidetoshi, Arahori, Hitomi, Wada, Kazuko, Kitabatake, Yasuji, Ozono, Keiichi

“…Chromosome abnormalities induces profound alterations in gene expression, leading to various disease phenotypes. Recent studies on yeast and mammalian cells…”
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Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities by Banno, Kimihiko, Omori, Sayaka, Hirata, Katsuya, Nawa, Nobutoshi, Nakagawa, Natsuki, Nishimura, Ken, Ohtaka, Manami, Nakanishi, Mahito, Sakuma, Tetsushi, Yamamoto, Takashi, Toki, Tsutomu, Ito, Etsuro, Yamamoto, Toshiyuki, Kokubu, Chikara, Takeda, Junji, Taniguchi, Hidetoshi, Arahori, Hitomi, Wada, Kazuko, Kitabatake, Yasuji, Ozono, Keiichi

“…Chromosomal aneuploidy and specific gene mutations are recognized early hallmarks of many oncogenic processes. However, the net effect of these abnormalities…”
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Conditional inactivation of Has2 reveals a crucial role for hyaluronan in skeletal growth, patterning, chondrocyte maturation and joint formation in the developing limb by Matsumoto, Kazu, Li, Yingcui, Jakuba, Caroline, Sugiyama, Yoshinori, Sayo, Tetsuya, Okuno, Misako, Dealy, Caroline N, Toole, Bryan P, Takeda, Junji, Yamaguchi, Yu, Kosher, Robert A

“…The glycosaminoglycan hyaluronan (HA) is a structural component of extracellular matrices and also interacts with cell surface receptors to directly influence…”
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COSMOS: accurate detection of somatic structural variations through asymmetric comparison between tumor and normal samples by Yamagata, Koichi, Yamanishi, Ayako, Kokubu, Chikara, Takeda, Junji, Sese, Jun

“…An important challenge in cancer genomics is precise detection of structural variations (SVs) by high-throughput short-read sequencing, which is hampered by…”
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Disruption of Stat3 reveals a critical role in both the initiation and the promotion stages of epithelial carcinogenesis by Chan, Keith Syson, Sano, Shigetoshi, Kiguchi, Kaoru, Anders, Joanne, Komazawa, Nobuyasu, Takeda, Junji, DiGiovanni, John

“…Constitutive activation of signal transducer and activator of transcription 3 (Stat3) has been found in a wide spectrum of human malignancies. Here, we have…”
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Variable SATB1 Levels Regulate Hematopoietic Stem Cell Heterogeneity with Distinct Lineage Fate by Doi, Yukiko, Yokota, Takafumi, Satoh, Yusuke, Okuzaki, Daisuke, Tokunaga, Masahiro, Ishibashi, Tomohiko, Sudo, Takao, Ueda, Tomoaki, Shingai, Yasuhiro, Ichii, Michiko, Tanimura, Akira, Ezoe, Sachiko, Shibayama, Hirohiko, Kohwi-Shigematsu, Terumi, Takeda, Junji, Oritani, Kenji, Kanakura, Yuzuru

“…Hematopoietic stem cells (HSCs) comprise a heterogeneous population exhibiting self-renewal and differentiation capabilities; however, the mechanisms involved…”
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Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis by Yamanishi, Ayako, Matsuba, Atsushi, Kondo, Ryohei, Akamatsu, Rie, Tanaka, Sachiyo, Tokunaga, Masahiro, Horie, Kyoji, Kokubu, Chikara, Ishida, Yasumasa, Takeda, Junji

“…Abstract Haploid mouse embryonic stem cells (ESCs), in which a single hit mutation is sufficient to produce loss-of-function phenotypes, have provided a…”
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