Search Results - "TAKAKURA, Yoshinobu"

Possibility of Exosome-Based Therapeutics and Challenges in Production of Exosomes Eligible for Therapeutic Application by Yamashita, Takuma, Takahashi, Yuki, Takakura, Yoshinobu

“…Exosomes are cell-derived vesicles with a diameter 30–120 nm. Exosomes contain endogenous proteins and nucleic acids; delivery of these molecules to…”
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Pharmacokinetics of Exosomes-An Important Factor for Elucidating the Biological Roles of Exosomes and for the Development of Exosome-Based Therapeutics by Morishita, Masaki, Takahashi, Yuki, Nishikawa, Makiya, Takakura, Yoshinobu

“…Exosomes are small membrane vesicles containing lipids, proteins, and nucleic acids. Recently, researchers have uncovered that exosomes are involved in various…”
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Therapeutic Application of Small Extracellular Vesicles (sEVs): Pharmaceutical and Pharmacokinetic Challenges by Takakura, Yoshinobu, Matsumoto, Akihiro, Takahashi, Yuki

“…Small extracellular vesicles (sEVs), including exosomes as typical example, are cell-derived vesicles comprising lipid bilayer with a diameter approximately…”
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Visualization and in vivo tracking of the exosomes of murine melanoma B16-BL6 cells in mice after intravenous injection by Takahashi, Yuki, Nishikawa, Makiya, Shinotsuka, Haruka, Matsui, Yuriko, Ohara, Saori, Imai, Takafumi, Takakura, Yoshinobu

“…•Gaussia luciferase-lactadherin fusion protein was designed to label exosomes.•Exosomes were successfully labeled with the fusion protein.•In vivo…”
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Cell type-specific and common characteristics of exosomes derived from mouse cell lines: Yield, physicochemical properties, and pharmacokinetics by Charoenviriyakul, Chonlada, Takahashi, Yuki, Morishita, Masaki, Matsumoto, Akihiro, Nishikawa, Makiya, Takakura, Yoshinobu

“…Exosomes are small membrane vesicles secreted from cells and are expected to be used as drug delivery systems. Important characteristics of exosomes, such as…”
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Antitumor immunity by small extracellular vesicles collected from activated dendritic cells through effective induction of cellular and humoral immune responses by Matsumoto, Akihiro, Asuka, Maho, Takahashi, Yuki, Takakura, Yoshinobu

“…Dendritic cell-derived small extracellular vesicles (DC-sEVs) are proposed as a novel candidate for tumor antigen-based cancer immunotherapy. In order to…”
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Development of allergic rhinitis immunotherapy using antigen-loaded small extracellular vesicles by Liu, Wen, Ota, Maki, Tabushi, Mayu, Takahashi, Yuki, Takakura, Yoshinobu

“…Allergic rhinitis is caused by a breakdown of the Th1/Th2 balance, in which the allergen-induced Th2 immune response predominates over the Th1 immune response,…”
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Effect of exosome isolation methods on physicochemical properties of exosomes and clearance of exosomes from the blood circulation by Yamashita, Takuma, Takahashi, Yuki, Nishikawa, Makiya, Takakura, Yoshinobu

“…[Display omitted] Exosomes, which are expected to be delivery systems for biomolecules such as nucleic acids, are collected by several methods. However, the…”
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Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells by Matsumoto, Akihiro, Takahashi, Yuki, Ariizumi, Reiichi, Nishikawa, Makiya, Takakura, Yoshinobu

“…Tumor-cell derived small extracellular vesicle (sEV) combined with immunostimulatory adjuvants may serve as a promising tumor vaccine through the induction of…”
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DNA nanotechnology-based composite-type gold nanoparticle-immunostimulatory DNA hydrogel for tumor photothermal immunotherapy by Yata, Tomoya, Takahashi, Yuki, Tan, Mengmeng, Nakatsuji, Hirotaka, Ohtsuki, Shozo, Murakami, Tatsuya, Imahori, Hiroshi, Umeki, Yuka, Shiomi, Tomoki, Takakura, Yoshinobu, Nishikawa, Makiya

“…Success of tumor photothermal immunotherapy requires a system that induces heat stress in cancer cells and enhances strong anti-tumor immune responses. Here,…”
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Quantitative analysis of tissue distribution of the B16BL6-derived exosomes using a streptavidin-lactadherin fusion protein and iodine-125-labeled biotin derivative after intravenous injection in mice by Morishita, Masaki, Takahashi, Yuki, Nishikawa, Makiya, Sano, Kohei, Kato, Kana, Yamashita, Takuma, Imai, Takafumi, Saji, Hideo, Takakura, Yoshinobu

“…We previously succeeded in the visualization of tissue distribution of B16BL6 cells-derived exosomes by labeling with Gaussia luciferase (gLuc)-LA, a fusion…”
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Enhanced Class I Tumor Antigen Presentation via Cytosolic Delivery of Exosomal Cargos by Tumor-Cell-Derived Exosomes Displaying a pH-Sensitive Fusogenic Peptide by Morishita, Masaki, Takahashi, Yuki, Nishikawa, Makiya, Ariizumi, Reiichi, Takakura, Yoshinobu

“…Tumor-cell-derived exosomes contain endogenous tumor antigens and can be used as a potential cancer vaccine without requiring identification of the…”
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Accelerated growth of B16BL6 tumor in mice through efficient uptake of their own exosomes by B16BL6 cells by Matsumoto, Akihiro, Takahashi, Yuki, Nishikawa, Makiya, Sano, Kohei, Morishita, Masaki, Charoenviriyakul, Chonlada, Saji, Hideo, Takakura, Yoshinobu

“…Exosomes are extracellular vesicles released by various cell types and play roles in cell–cell communication. Several studies indicate that cancer cell‐derived…”
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Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice by Nakamura, Yuto, Kita, Shunbun, Tanaka, Yoshimitsu, Fukuda, Shiro, Obata, Yoshinari, Okita, Tomonori, Nishida, Hiroyuki, Takahashi, Yuki, Kawachi, Yusuke, Tsugawa-Shimizu, Yuri, Fujishima, Yuya, Nishizawa, Hitoshi, Takakura, Yoshinobu, Miyagawa, Shigeru, Sawa, Yoshiki, Maeda, Norikazu, Shimomura, Iichiro

“…Mesenchymal stem/stromal cells (MSCs) are cultured adult stem cells that originally reside in virtually all tissues, and the gain of MSCs by transplantation…”
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Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply by Mizukami, Yuya, Takahashi, Yuki, Shimizu, Kazunori, Konishi, Satoshi, Takakura, Yoshinobu, Nishikawa, Makiya

“…Multicellular spheroids are expected to be used for in vivo-like tissue models and cell transplantation. Microwell devices are useful for the fabrication of…”
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Development of RNA/DNA Hydrogel Targeting Toll-Like Receptor 7/8 for Sustained RNA Release and Potent Immune Activation by Komura, Fusae, Okuzumi, Kana, Takahashi, Yuki, Takakura, Yoshinobu, Nishikawa, Makiya

“…Guanosine- and uridine-rich single-stranded RNA (GU-rich RNA) is an agonist of Toll-like receptor (TLR) 7 and TLR8 and induces strong immune responses. A…”
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Macrophage-dependent clearance of systemically administered B16BL6-derived exosomes from the blood circulation in mice by Imai, Takafumi, Takahashi, Yuki, Nishikawa, Makiya, Kato, Kana, Morishita, Masaki, Yamashita, Takuma, Matsumoto, Akihiro, Charoenviriyakul, Chonlada, Takakura, Yoshinobu

“…Previous studies using B16BL6-derived exosomes labelled with gLuc-lactadherin (gLuc-LA), a fusion protein of Gaussia luciferase (a reporter protein) and…”
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Effects of Localization of Antigen Proteins in Antigen-Loaded Exosomes on Efficiency of Antigen Presentation by Arima, Yuta, Liu, Wen, Takahashi, Yuki, Nishikawa, Makiya, Takakura, Yoshinobu

“…Exosomes are considered to be vehicles of antigen delivery. The localization of antigen proteins, i.e., whether they lie on the outer surface or inner surface…”
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Optimization of Albumin Secretion and Metabolic Activity of Cytochrome P450 1A1 of Human Hepatoblastoma HepG2 Cells in Multicellular Spheroids by Controlling Spheroid Size by Nishikawa, Tomoko, Tanaka, Yutaro, Nishikawa, Makiya, Ogino, Yuka, Kusamori, Kosuke, Mizuno, Narumi, Mizukami, Yuya, Shimizu, Kazunori, Konishi, Satoshi, Takahashi, Yuki, Takakura, Yoshinobu

“…Multicellular spheroids are useful as three-dimensional cell culture systems and for cell-based therapies. Their successful application requires an…”
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In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase by Takahashi, Yuki, Nishikawa, Makiya, Takakura, Yoshinobu

“…Extracellular vesicles (EVs) are cell-derived vesicles comprising a lipid bilayer and are found in body fluids, such as blood, sweat, and urine. As EVs,…”
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