Search Results - "T. Carrondo, Manuel J."

Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells by Serra, Margarida, Correia, Cláudia, Malpique, Rita, Brito, Catarina, Jensen, Janne, Bjorquist, Petter, Carrondo, Manuel J T, Alves, Paula M

“…The successful implementation of human embryonic stem cells (hESCs)-based technologies requires the production of relevant numbers of well-characterized cells…”
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Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing by Tostões, Rui M., Leite, Sofia B., Serra, Margarida, Jensen, Janne, Björquist, Petter, Carrondo, Manuel J. T., Brito, Catarina, Alves, Paula M.

“…Primary cultures of human hepatocyte spheroids are a promising in vitro model for long‐term studies of hepatic metabolism and cytotoxicity. The lack of robust…”
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Improved virus purification processes for vaccines and gene therapy by Nestola, Piergiuseppe, Peixoto, Cristina, Silva, Ricardo R. J. S, Alves, Paula M., Mota, José P. B., Carrondo, Manuel J. T.

“…ABSTRACT The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving…”
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Metabolic responses of CHO cells to limitation of key amino acids by Duarte, Tiago M., Carinhas, Nuno, Barreiro, Laura C., Carrondo, Manuel J. T., Alves, Paula M., Teixeira, Ana P.

“…ABSTRACT Chinese hamster ovary (CHO) cells are the predominant host for production of therapeutic glycoproteins. In particular, the glutamine‐synthetase (GS)…”
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Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes by Correia, Cláudia, Serra, Margarida, Espinha, Nuno, Sousa, Marcos, Brito, Catarina, Burkert, Karsten, Zheng, Yunjie, Hescheler, Jürgen, Carrondo, Manuel J. T., Šarić, Tomo, Alves, Paula M.

“…Cardiomyocytes (CMs) derived from induced pluripotent stem cells (iPSCs) hold great promise for patient-specific disease modeling, drug screening and cell…”
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A detection and quantification label-free tool to speed up downstream processing of model mucins by Carvalho, Sofia B, Moreira, Ana Sofia, Gomes, Joana, Carrondo, Manuel J T, Thornton, David J, Alves, Paula M, Costa, Julia, Peixoto, Cristina

“…Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for…”
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Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells by Vasconcelos E Sá, João, Simão, Daniel, Terrasso, Ana P, Silva, Marta M, Brito, Catarina, Isidro, Inês A, Alves, Paula M, Carrondo, Manuel J T

“…Metabolism plays an essential role in cell fate decisions. However, the methods used for metabolic characterization and for finding potential metabolic…”
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Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography by Nestola, Piergiuseppe, Silva, Ricardo J.S., Peixoto, Cristina, Alves, Paula M., Carrondo, Manuel J.T., Mota, José P.B.

“…•Ad5 was purified by size-exclusion chromatography (SEC) in a 2-column open-loop SMB system.•Clearance of impurities, namely DNA and host cell protein (HCP),…”
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Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange by Carinhas, Nuno, Bernal, Vicente, Yokomizo, Adriana Y., Carrondo, Manuel J. T., Oliveira, Rui, Alves, Paula M.

“…One of the major concerns regarding the use of insect cells and baculovirus expression vectors for the production of recombinant proteins is the drop in…”
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Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control by Serra, Margarida, Brito, Catarina, Sousa, Marcos F.Q., Jensen, Janne, Tostões, Rui, Clemente, João, Strehl, Raimund, Hyllner, Johan, Carrondo, Manuel J.T., Alves, Paula M.

“…The successful transfer of human embryonic stem cell (hESC) technology and cellular products into clinical and industrial applications needs to address issues…”
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Cell density effect in the baculovirus-insect cells system: A quantitative analysis of energetic metabolism by Bernal, Vicente, Carinhas, Nuno, Yokomizo, Adriana Y, Carrondo, Manuel J.T, Alves, Paula M

“…The cell density effect (i.e., the drop in the specific productivity in the baculovirus-insect cells expression system when cells are infected at high cell…”
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Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration by Nestola, Piergiuseppe, Martins, Duarte L, Peixoto, Cristina, Roederstein, Susanne, Schleuss, Tobias, Alves, Paula M, Mota, José P B, Carrondo, Manuel J T

“…The purification of virus particles and viral vectors for vaccine and gene therapy applications is gaining increasing importance in order to deliver a fast,…”
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Systems biotechnology of animal cells: the road to prediction by Carinhas, Nuno, Oliveira, Rui, Alves, Paula Marques, Carrondo, Manuel J.T, Teixeira, Ana P

“…A central concern of biopharmaceutical R&D is the production of sufficient quantities of recombinant products from manufacturing processes based on animal cell…”
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Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles by Carvalho, Sofia B, Freire, João M, Moleirinho, Mafalda G, Monteiro, Francisca, Gaspar, Diana, Castanho, Miguel A. R. B, Carrondo, Manuel J. T, Alves, Paula M, Bernardes, Gonçalo J. L, Peixoto, Cristina

“…Virus-like particles (VLPs) constitute a promising platform in vaccine development and targeted drug delivery. To date, most applications use simple…”
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Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells by Rodrigues, Ana F, Carrondo, Manuel J.T, Alves, Paula M, Coroadinha, Ana S

“…Highlights • Vaccines and viral vectors produced in animal cells constitute a growing research field and market. • Virus–host interactions can unveil targets…”
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Perfusion of 3D encapsulated hepatocytes—A synergistic effect enhancing long‐term functionality in bioreactors by Tostões, Rui M, Leite, Sofia B, Miranda, Joana P, Sousa, Marcos, Wang, Daniel I.C, Carrondo, Manuel J.T, Alves, Paula M

“…Long‐term primary cultures of hepatocytes are essential for bioartificial liver (BAL) devices and to reduce and replace animal tests in lead candidate…”
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Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control by Teixeira, Ana P, Alves, Carlos, Alves, Paula M, Carrondo, Manuel J T, Oliveira, Rui

“…The progress in the "-omic" sciences has allowed a deeper knowledge on many biological systems with industrial interest. This knowledge is still rarely used…”
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Insect cells as a production platform of complex virus-like particles by Fernandes, Fabiana, Teixeira, Ana P, Carinhas, Nuno, Carrondo, Manuel J T, Alves, Paula M

“…Virus-like particles (VLPs) are multiprotein structures that resemble the conformation of native viruses but lack a viral genome, potentiating their…”
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Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia by Silva, Ana Carina, Yami, Martha, Libeau, Geneviève, Carrondo, Manuel J.T, Alves, Paula M

“…Abstract In this paper extended tests on a new candidate formulation for Peste des Petits Ruminants (PPR) vaccine carried out at National Veterinary Institute…”
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How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology? by Carrondo, Manuel J. T., Alves, Paula M., Carinhas, Nuno, Glassey, Jarka, Hesse, Friedemann, Merten, Otto-Wilhelm, Micheletti, Martina, Noll, Thomas, Oliveira, Rui, Reichl, Udo, Staby, Arne, Teixeira, Ana P., Weichert, Henry, Mandenius, Carl-Fredrik

“…This report highlights the potential of measurement, monitoring, modeling and control (M3C) methodologies in animal and human cell culture technology. In…”
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