IR and Raman imaging of murine brains from control and ApoE/LDLR(-/-) mice with advanced atherosclerosis

Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional br...

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Bibliographic Details
Published in:	Analyst (London) Vol. 141; no. 18; p. 5329
Main Authors:	Kochan, Kamila, Chrabaszcz, Karolina, Szczur, Barbara, Maslak, Edyta, Dybas, Jakub, Marzec, Katarzyna M
Format:	Journal Article
Language:	English
Published:	England 21-09-2016
Subjects:	Animals Atherosclerosis - diagnostic imaging Brain - diagnostic imaging Mice Mice, Inbred C57BL Mice, Knockout, ApoE Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman
Online Access:	Get more information
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Summary:	Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR(-/-) mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.
ISSN:	1364-5528
DOI:	10.1039/c6an00107f