Time Resolved Cryo‐Correlative Light and Electron Microscopy
Complex materials exhibit fascinating features especially in situations far from equilibrium. Thus, methods for investigating structural dynamics with sub‐second time resolution are becoming a question of interest at varying spatial scales. With novel microscopy techniques steadily improving, the te...
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| Published in: | Advanced functional materials Vol. 34; no. 30 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Hoboken
Wiley Subscription Services, Inc
01-07-2024
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Complex materials exhibit fascinating features especially in situations far from equilibrium. Thus, methods for investigating structural dynamics with sub‐second time resolution are becoming a question of interest at varying spatial scales. With novel microscopy techniques steadily improving, the temporal and spatial limits of multiple imaging methods are investigated with an emphasis on the important role of correlative imaging and cryo‐fixation. A deep‐dive is taken into cryo‐correlative light and electron microscopy (CLEM) as a starting point for multimodal investigations of ultrastructural dynamics at high spatiotemporal resolution. The focus is on highlighting the different microscopy methods that capture the following key aspects: 1) samples are as close to native state as possible 2) dynamic process information is captured, 3) high structural resolution is enabled. Additionally, the size of samples that can be imaged under these conditions is looked at and approaches not only focusing on single molecules, but larger structures are highlighted.
The development of cryo‐EM is a breakthrough in structural biology, and the method is increasingly being used in materials science to study new battery materials, drug formulations, and other systems. However, studying a variety of important dynamic processes with cryomicroscopy is challenging. To address this challenge, new time‐resolved methods have emerged in recent years. In this perspective, an overview of these developments is given with a special focus on time resolved correlative light and electron microscopy (CLEM) at low temperature. |
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| ISSN: | 1616-301X 1616-3028 |
| DOI: | 10.1002/adfm.202313705 |