Search Results - "Sykes, Brian D."

Targeting the sarcomere to correct muscle function by Hwang, Peter M., Sykes, Brian D.

“…Key Points The sarcomere is the most basic contractile unit in skeletal and cardiac muscle. Mutations in genes encoding sarcomeric proteins give rise to…”
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Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli by Hwang, Peter M., Pan, Jonathan S., Sykes, Brian D.

“…Today, proteins are typically overexpressed using solubility-enhancing fusion tags that allow for affinity chromatographic purification and subsequent removal…”
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Structural and functional analysis of the Na+/H+ exchanger by Slepkov, Emily R, Rainey, Jan K, Sykes, Brian D, Fliegel, Larry

“…The mammalian NHE (Na+/H+ exchanger) is a ubiquitously expressed integral membrane protein that regulates intracellular pH by removing a proton in exchange for…”
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Investigations of the Effects of Gender, Diurnal Variation, and Age in Human Urinary Metabolomic Profiles by Slupsky, Carolyn M, Rankin, Kathryn N, Wagner, James, Fu, Hao, Chang, David, Weljie, Aalim M, Saude, Erik J, Lix, Bruce, Adamko, Darryl J, Shah, Sirish, Greiner, Russ, Sykes, Brian D, Marrie, Thomas J

“…Metabolomics may have the capacity to revolutionize disease diagnosis through the identification of scores of metabolites that vary during environmental,…”
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cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C by Hwang, Peter M., Cai, Fangze, Pineda-Sanabria, Sandra E., Corson, David C., Sykes, Brian D.

“…The cardiac isoform of troponin I (cTnI) has a unique 31-residue N-terminal region that binds cardiac troponin C (cTnC) to increase the calcium sensitivity of…”
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Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy by Saude, Erik J., MD, PhD, Skappak, Christopher D, Regush, Shana, BSc, Cook, Kim, RN, Ben-Zvi, Amos, PEng, PhD, Becker, Allan, MD, FRCPC, Moqbel, Redwan, PhD, FRCPath, Sykes, Brian D., PhD, Rowe, Brian H., MD, MSc, CCFP(EM), Adamko, Darryl J., MD, FRCPC

“…Background The ability to diagnose and monitor asthma on the basis of noninvasive measurements of airway cellular dysfunction is difficult in the typical…”
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Urine stability for metabolomic studies: effects of preparation and storage by Saude, Erik J, Sykes, Brian D

“…Metabolomic studies attempt to identify and profile unique metabolic differences among test populations, which may be correlated with a specific biological…”
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Rewiring AMPK and Mitochondrial Retrograde Signaling for Metabolic Control of Aging and Histone Acetylation in Respiratory-Defective Cells by Friis, R. Magnus N., Glaves, John Paul, Huan, Tao, Li, Liang, Sykes, Brian D., Schultz, Michael C.

“…Abnormal respiratory metabolism plays a role in numerous human disorders. We find that regulation of overall histone acetylation is perturbed in…”
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Solution Structure of Human Cardiac Troponin C in Complex with the Green Tea Polyphenol, (−)-Epigallocatechin 3-Gallate by Robertson, Ian M., Li, Monica X., Sykes, Brian D.

“…Heart muscle contraction is regulated by Ca2+ binding to the thin filament protein troponin C. In cardiovascular disease, the myofilament response to Ca2+ is…”
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A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli by Hwang, Peter M., Pan, Jonathan S., Sykes, Brian D.

“…► We developed a fusion protein expression system based on the membrane protein, PagP. ► The new system shows improved expression and targeting to inclusion…”
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Ubiquinone-binding Site Mutations in the Saccharomyces cerevisiae Succinate Dehydrogenase Generate Superoxide and Lead to the Accumulation of Succinate by Szeto, Samuel S.W., Reinke, Stacey N., Sykes, Brian D., Lemire, Bernard D.

“…The mitochondrial succinate dehydrogenase (SDH) is an essential component of the electron transport chain and of the tricarboxylic acid cycle. Also known as…”
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A Common Phenotype Polymorphism in Mammalian Brains Defined by Concomitant Production of Prolactin and Growth Hormone by Daude, Nathalie, Lee, Inyoul, Kim, Taek-Kyun, Janus, Christopher, Glaves, John Paul, Gapeshina, Hristina, Yang, Jing, Sykes, Brian D, Carlson, George A, Hood, Leroy E, Westaway, David

“…Pituitary Prolactin (PRL) and Growth Hormone (GH) are separately controlled and sub-serve different purposes. Surprisingly, we demonstrate that extra-pituitary…”
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Structural and Functional Characterization of Transmembrane Segment VII of the Na+/H+ Exchanger Isoform 1 by Ding, Jie, Rainey, Jan K., Xu, Caroline, Sykes, Brian D., Fliegel, Larry

“…The Na+/H+ exchanger isoform 1 is an integral membrane protein that regulates intracellular pH by exchanging one intracellular H+ for one extracellular Na+. It…”
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Binding of Cardiac Troponin-I147-163 Induces a Structural Opening in Human Cardiac Troponin-C by Li, Monica X, Spyracopoulos, Leo, Sykes, Brian D

“…The interaction of troponin-C (TnC) with troponin-I (TnI) plays a central role in skeletal and cardiac muscle contraction. We have recently shown that the…”
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Structural and Functional Analysis of Transmembrane XI of the NHE1 Isoform of the Na+/H+ Exchanger by Lee, Brian L., Li, Xiuju, Liu, Yongsheng, Sykes, Brian D., Fliegel, Larry

“…The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals by extruding an intracellular…”
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DNA Binding: a Novel Function of Pseudomonas aeruginosa Type IV Pili by VAN SCHAIK, Erin J, GILTNER, Carmen L, AUDETTE, Gerald F, KEIZER, David W, BAUTISTA, Daisy L, SLUPSKY, Carolyn M, SYKES, Brian D, IRVIN, Randall T

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Solution Structure of a DNA Duplex Containing the Potent Anti-Poxvirus Agent Cidofovir by Julien, Olivier, Beadle, James R, Magee, Wendy C, Chatterjee, Subhrangsu, Hostetler, Karl Y, Evans, David H, Sykes, Brian D

“…Cidofovir (1(S)-[3-hydroxy-2-(phosphonome-thoxy)propyl]cytosine, CDV) is a potent inhibitor of orthopoxvirus DNA replication. Prior studies have shown that,…”
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Structural and Functional Characterization of Transmembrane Segment IX of the NHE1 Isoform of the Na+/H+ Exchanger by Reddy, Tyler, Ding, Jie, Li, Xiuju, Sykes, Brian D., Rainey, Jan K., Fliegel, Larry

“…The Na+/H+ exchanger isoform 1 (NHE1) is an integral membrane protein that regulates intracellular pH by removing one intracellular H+ in exchange for one…”
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Relative and Regional Stabilities of the Hamster, Mouse, Rabbit, and Bovine Prion Proteins toward Urea Unfolding Assessed by Nuclear Magnetic Resonance and Circular Dichroism Spectroscopies by Julien, Olivier, Chatterjee, Subhrangsu, Bjorndahl, Trent C, Sweeting, Braden, Acharya, Sandipta, Semenchenko, Valentyna, Chakrabartty, Avijit, Pai, Emil F, Wishart, David S, Sykes, Brian D, Cashman, Neil R

“…The residue-specific urea-induced unfolding patterns of recombinant prion proteins from different species (bovine, rabbit, mouse, and Syrian hamster) were…”
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Fluorine labelling for in situ 19F NMR in oriented systems by Cockburn, Kieran T., Sykes, Brian D.

“…The focus of this project is to take advantage of the large NMR chemical shift anisotropy of 19 F to determine the orientation of fluorine labeled biomolecules…”
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