Search Results - "Syka, John E. P."

Enhanced Sequence Coverage of Large Proteins by Combining Ultraviolet Photodissociation with Proton Transfer Reactions by Sanders, James D, Mullen, Christopher, Watts, Eleanor, Holden, Dustin D, Syka, John E. P, Schwartz, Jae C, Brodbelt, Jennifer S

“…Ultraviolet photodissociation (UVPD) produces rich and informative fragmentation of intact protein ions, but in the case of high mass proteins (>30 kDa) the…”
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Supplemental Activation Method for High-Efficiency Electron-Transfer Dissociation of Doubly Protonated Peptide Precursors by Swaney, Danielle L, McAlister, Graeme C, Wirtala, Matthew, Schwartz, Jae C, Syka, John E. P, Coon, Joshua J

“…Electron-transfer dissociation (ETD) delivers the unique attributes of electron capture dissociation to mass spectrometers that utilize radio frequency…”
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Exposing the molecular heterogeneity of glycosylated biotherapeutics by Schachner, Luis F., Mullen, Christopher, Phung, Wilson, Hinkle, Joshua D., Beardsley, Michelle Irwin, Bentley, Tracy, Day, Peter, Tsai, Christina, Sukumaran, Siddharth, Baginski, Tomasz, DiCara, Danielle, Agard, Nicholas J., Masureel, Matthieu, Gober, Joshua, ElSohly, Adel M., Melani, Rafael, Syka, John E. P., Huguet, Romain, Marty, Michael T., Sandoval, Wendy

“…The heterogeneity inherent in today’s biotherapeutics, especially as a result of heavy glycosylation, can affect a molecule’s safety and efficacy…”
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Rapid MRSA detection via tandem mass spectrometry of the intact 80 kDa PBP2a resistance protein by Neil, Jason R., Verma, Arvind, Kronewitter, Scott R., McGee, William M., Mullen, Christopher, Viirtola, Marjaana, Kotovuori, Annika, Friedrich, Herdis, Finell, Johan, Rannisto, Joni, Syka, John E. P., Stephenson, James L.

“…Treatment of antibiotic-resistant infections is dependent on the detection of specific bacterial genes or proteins in clinical assays. Identification of…”
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Analysis of Phosphorylation Sites on Proteins from Saccharomyces cerevisiae by Electron Transfer Dissociation (ETD) Mass Spectrometry by Chi, An, Huttenhower, Curtis, Geer, Lewis Y., Coon, Joshua J., Syka, John E. P., Bai, Dina L., Shabanowitz, Jeffrey, Burke, Daniel J., Troyanskaya, Olga G., Hunt, Donald F.

“…We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography…”
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Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis by Weisbrod, Chad R., Kaiser, Nathan K., Syka, John E. P., Early, Lee, Mullen, Christopher, Dunyach, Jean-Jacques, English, A. Michelle, Anderson, Lissa C., Blakney, Greg T., Shabanowitz, Jeffrey, Hendrickson, Christopher L., Marshall, Alan G., Hunt, Donald F.

“…High resolution mass spectrometry is a key technology for in-depth protein characterization. High-field Fourier transform ion cyclotron resonance mass…”
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Front-End Electron Transfer Dissociation: A New Ionization Source by Earley, Lee, Anderson, Lissa C, Bai, Dina L, Mullen, Christopher, Syka, John E. P, English, A. Michelle, Dunyach, Jean-Jacques, Stafford, George C, Shabanowitz, Jeffrey, Hunt, Donald F, Compton, Philip D

“…Electron transfer dissociation (ETD), a technique that provides efficient fragmentation while depositing little energy into vibrational modes, has been widely…”
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A two-dimensional quadrupole ion trap mass spectrometer by Schwartz, Jae C, Senko, Michael W, Syka, John E.P

“…The use of a linear or two-dimensional (2-D) quadrupole ion trap as a high performance mass spectrometer is demonstrated. Mass analysis is performed by…”
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Protein Identification Using Sequential Ion/Ion Reactions and Tandem Mass Spectrometry by Coon, Joshua J., Ueberheide, Beatrix, John E. P. Syka, Dryhurst, Deanna D., Ausio, Juan, Shabanowitz, Jeffrey, Hunt, Donald F., McLafferty, Fred W.

“…A method for rapid sequencing of intact proteins simultaneously from the N and C termini (1-2 s) with online chromatography is described and applied to the…”
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Electron Transfer Dissociation of Peptide Anions by Coon, Joshua J., Shabanowitz, Jeffrey, Hunt, Donald F., Syka, John E.P.

“…Ion/ion reactions of multiply deprotonated peptide anions with xenon radical cations result in electron abstraction to generate charge-reduced peptide anions…”
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Novel Linear Quadrupole Ion Trap/FT Mass Spectrometer:  Performance Characterization and Use in the Comparative Analysis of Histone H3 Post-translational Modifications by Syka, John E. P, Marto, Jarrod A, Bai, Dina L, Horning, Stevan, Senko, Michael W, Schwartz, Jae C, Ueberheide, Beatrix, Garcia, Benjamin, Busby, Scott, Muratore, Tara, Shabanowitz, Jeffrey, Hunt, Donald F

“…We describe the design and performance of a prototype high performance hybrid mass spectrometer. This instrument consists of a linear quadrupole ion trap (QLT)…”
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Infrared Photoactivation Reduces Peptide Folding and Hydrogen-Atom Migration following ETD Tandem Mass Spectrometry by Ledvina, Aaron R, McAlister, Graeme C, Gardner, Myles W, Smith, Suncerae I, Madsen, James A, Schwartz, Jae C, Stafford, George C. Jr, Syka, John E.P, Brodbelt, Jennifer S, Coon, Joshua J

“…Peptides see the light: The photoactivation of peptide precursor cations during electron transfer dissociation (ETD) led to the generation of isotopic cluster…”
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Proton Transfer Charge Reduction Enables High-Throughput Top-Down Analysis of Large Proteoforms by Huguet, Romain, Mullen, Christopher, Srzentić, Kristina, Greer, Joseph B, Fellers, Ryan T, Zabrouskov, Vlad, Syka, John E. P, Kelleher, Neil L, Fornelli, Luca

“…Despite the recent technological advances in Fourier transform mass spectrometry (FTMS) instrumentation, top-down proteomics (TDP) is currently mostly applied…”
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Sequential Ion–Ion Reactions for Enhanced Gas-Phase Sequencing of Large Intact Proteins in a Tribrid Orbitrap Mass Spectrometer by Kline, Jake T, Mullen, Christopher, Durbin, Kenneth R, Oates, Ryan N, Huguet, Romain, Syka, John E. P, Fornelli, Luca

“…Obtaining extensive sequencing of an intact protein is essential in order to simultaneously determine both the nature and exact localization of chemical and…”
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Ion–Ion Charge Reduction Addresses Multiple Challenges Common to Denaturing Intact Mass Analysis by Bailey, Aaron O, Huguet, Romain, Mullen, Christopher, Syka, John E. P, Russell, William K

“…Complete LC–MS-based protein primary sequence characterization requires measurement of intact protein profiles under denaturing and/or reducing conditions. To…”
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Towards a universal method for middle-down analysis of antibodies via proton transfer charge reduction—Orbitrap mass spectrometry by Oates, Ryan N., Lieu, Linda B., Kline, Jake T., Mullen, Christopher, Srzentić, Kristina, Huguet, Romain, McAlister, Graeme C., Huang, Jingjing, Bergen, David, Melani, Rafael D., Zabrouskov, Vlad, Durbin, Kenneth R., Syka, John E. P., Fornelli, Luca

“…Modern mass spectrometry technology allows for extensive sequencing of the ~ 25 kDa subunits of monoclonal antibodies (mAbs) produced by IdeS proteolysis…”
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Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins by Gault, Joseph, Liko, Idlir, Landreh, Michael, Shutin, Denis, Bolla, Jani Reddy, Jefferies, Damien, Agasid, Mark, Yen, Hsin-Yung, Ladds, Marcus J. G. W., Lane, David P., Khalid, Syma, Mullen, Christopher, Remes, Philip M., Huguet, Romain, McAlister, Graeme, Goodwin, Michael, Viner, Rosa, Syka, John E.P., Robinson, Carol V.

“…Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down…”
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Peptide and Protein Sequence Analysis by Electron Transfer Dissociation Mass Spectrometry by John E. P. Syka, Coon, Joshua J., Schroeder, Melanie J., Shabanowitz, Jeffrey, Hunt, Donald F., McLafferty, Fred W.

“…Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene…”
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Infrared Photoactivation Boosts Reporter Ion Yield in Isobaric Tagging by Lee, Kenneth W, Peters-Clarke, Trenton M, Mertz, Keaton L, McAlister, Graeme C, Syka, John E. P, Westphall, Michael S, Coon, Joshua J

“…Isobaric tagging facilitates multiplexed experiments that can determine sequences and relative amounts of peptides in biological samples using tandem mass…”
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Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation by Duselis, Elizabeth M, Panepinto, Maria C, Syka, John E.P, Mullen, Christopher, D’Ippolito, Robert A, English, A. Michelle, Ugrin, Scott A, Shabanowitz, Jeffrey, Hunt, Donald F

“…Electron transfer dissociation (ETD) is an analytically useful tool for primary structure interrogation of intact proteins, but its utility is limited by…”
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