Search Results - "Suzuki, Ken‐Ichi"

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 by Nakade, Shota, Tsubota, Takuya, Sakane, Yuto, Kume, Satoshi, Sakamoto, Naoaki, Obara, Masanobu, Daimon, Takaaki, Sezutsu, Hideki, Yamamoto, Takashi, Sakuma, Tetsushi, Suzuki, Ken-ichi T.

“…Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting…”
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Fgf10 mutant newts regenerate normal hindlimbs despite severe developmental defects by Suzuki, Miyuki, Okumura, Akinori, Chihara, Akane, Shibata, Yuki, Endo, Tetsuya, Teramoto, Machiko, Agata, Kiyokazu, Bronner, Marianne E, Suzuki, Ken-Ichi T

“…In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function is broadly conserved in tetrapods and/or…”
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A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis by Mochii, Makoto, Akizuki, Kai, Ossaka, Hero, Kagawa, Norie, Umesono, Yoshihiko, Suzuki, Ken-Ichi T

“…Xenopus laevis is a widely used model organism in developmental and regeneration studies. Despite several reports regarding targeted integration techniques in…”
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CRISPR-Cas9-Based Functional Analysis in Amphibians: Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl by Suzuki, Miyuki, Iida, Midori, Hayashi, Toshinori, Suzuki, Ken-Ichi T

“…Amphibians have made many fundamental contributions to our knowledge, from basic biology to biomedical research on human diseases. Current genome editing tools…”
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Efficient TALEN construction and evaluation methods for human cell and animal applications by Sakuma, Tetsushi, Hosoi, Sayaka, Woltjen, Knut, Suzuki, Ken‐ichi, Kashiwagi, Keiko, Wada, Housei, Ochiai, Hiroshi, Miyamoto, Tatsuo, Kawai, Narudo, Sasakura, Yasunori, Matsuura, Shinya, Okada, Yasushi, Kawahara, Atsuo, Hayashi, Shigeo, Yamamoto, Takashi

“…Transcription activator–like effector nucleases (TALENs) have recently arisen as effective tools for targeted genome engineering. Here, we report streamlined…”
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A simple and practical workflow for genotyping of CRISPR–Cas9‐based knockout phenotypes using multiplexed amplicon sequencing by Iida, Midori, Suzuki, Miyuki, Sakane, Yuto, Nishide, Hiroyo, Uchiyama, Ikuo, Yamamoto, Takashi, Suzuki, Ken‐ichi T., Fujii, Satoshi

“…Founder animals carrying high proportions of somatic mutation induced by CRISPR–Cas9 enable a rapid and scalable strategy for the functional screening of…”
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hoxc12/c13 as key regulators for rebooting the developmental program in Xenopus limb regeneration by Kawasumi-Kita, Aiko, Lee, Sang-Woo, Ohtsuka, Daisuke, Niimi, Kaori, Asakura, Yoshifumi, Kitajima, Keiichi, Sakane, Yuto, Tamura, Koji, Ochi, Haruki, Suzuki, Ken-ichi T., Morishita, Yoshihiro

“…During organ regeneration, after the initial responses to injury, gene expression patterns similar to those in normal development are reestablished during…”
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CRISPR/Cas9-based simple transgenesis in Xenopus laevis by Shibata, Yuki, Suzuki, Miyuki, Hirose, Nao, Takayama, Ayuko, Sanbo, Chiaki, Inoue, Takeshi, Umesono, Yoshihiko, Agata, Kiyokazu, Ueno, Naoto, Suzuki, Ken-ichi T., Mochii, Makoto

“…Transgenic techniques have greatly increased our understanding of the transcriptional regulation of target genes through live reporter imaging, as well as the…”
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Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration by Suzuki, Miyuki, Hayashi, Toshinori, Inoue, Takeshi, Agata, Kiyokazu, Hirayama, Miki, Suzuki, Miyuzu, Shigenobu, Shuji, Takeuchi, Takashi, Yamamoto, Takashi, Suzuki, Ken-ichi T.

“…Newts have remarkable ability to regenerate their organs and have been used in research for centuries. However, the laborious work of breeding has hampered…”
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Rapid and efficient analysis of gene function using CRISPR‐Cas9 in Xenopus tropicalis founders by Shigeta, Mitsuki, Sakane, Yuto, Iida, Midori, Suzuki, Miyuki, Kashiwagi, Keiko, Kashiwagi, Akihiko, Fujii, Satoshi, Yamamoto, Takashi, Suzuki, Ken‐ichi T.

“…Recent advances in genome editing using programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator‐like effector nucleases (TALENs)…”
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Newt Hoxa13 has an essential and predominant role in digit formation during development and regeneration by Takeuchi, Takashi, Matsubara, Haruka, Minamitani, Fumina, Satoh, Yukio, Tozawa, Sayo, Moriyama, Tomoki, Maruyama, Kohei, Suzuki, Ken-Ichi T, Shigenobu, Shuji, Inoue, Takeshi, Tamura, Koji, Agata, Kiyokazu, Hayashi, Toshinori

“…The 5'Hox genes play crucial roles in limb development and specify regions in the proximal-distal axis of limbs. However, there is no direct genetic evidence…”
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A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology by Matsunami, Masatoshi, Suzuki, Miyuki, Haramoto, Yoshikazu, Fukui, Akimasa, Inoue, Takeshi, Yamaguchi, Katsushi, Uchiyama, Ikuo, Mori, Kazuki, Tashiro, Kosuke, Ito, Yuzuru, Takeuchi, Takashi, Suzuki, Ken-Ichi T, Agata, Kiyokazu, Shigenobu, Shuji, Hayashi, Toshinori

“…Urodele newts have unique biological properties, notably including prominent regeneration ability. The Iberian ribbed newt, Pleurodeles waltl, is a promising…”
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Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung by Habuta, Munenori, Yasue, Akihiro, Suzuki, Ken-Ichi T, Fujita, Hirofumi, Sato, Keita, Kono, Hitomi, Takayama, Ayuko, Bando, Tetsuya, Miyaishi, Satoru, Oyadomari, Seiichi, Tanaka, Eiji, Ohuchi, Hideyo

“…CRISPR/Cas9-mediated gene editing often generates founder generation (F0) mice that exhibit somatic mosaicism in the targeted gene(s). It has been known that…”
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Improvement in Processability for Injection Molding of Bisphenol-A Polycarbonate by Addition of Low-Density Polyethylene by Kuroda, Yuki, Suzuki, Ken-Ichi, Kikuchi, Genzo, Moonprasith, Nantina, Kida, Takumitsu, Yamaguchi, Masayuki

“…The rheological properties and processability at injection molding were studied for bisphenol-A polycarbonate (PC) that was modified by low-density…”
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Cilia play a role in breaking left–right symmetry of the sea urchin embryo by Takemoto, Ayumi, Miyamoto, Tatsuo, Simono, Fumie, Kurogi, Nao, Shirae‐Kurabayashi, Maki, Awazu, Akinori, Suzuki, Ken‐ichi T., Yamamoto, Takashi, Sakamoto, Naoaki

“…Left–right asymmetry of bilaterian animals is established during early development. In mice, frogs and fishes, the ciliated left–right organizer plays an…”
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Amiodarone bioconcentration and suppression of metamorphosis in Xenopus by Sanoh, Seigo, Hanada, Hideki, Kashiwagi, Keiko, Mori, Tsukasa, Goto-Inoue, Naoko, Suzuki, Ken-ichi T., Mori, Junpei, Nakamura, Naoki, Yamamoto, Takashi, Kitamura, Shigeyuki, Kotake, Yaichiro, Sugihara, Kazumi, Ohta, Shigeru, Kashiwagi, Akihiko

“…•This study examines amiodarone (AMD) bioconcentration and metamorphosis in Xenopus.•AMD acts as a competitive antagonist to thyroid hormone (TH) action.•This…”
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The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos by Miyamoto, Kei, Suzuki, Ken-Ichi T, Suzuki, Miyuki, Sakane, Yuto, Sakuma, Tetsushi, Herberg, Sarah, Simeone, Angela, Simpson, David, Jullien, Jerome, Yamamoto, Takashi, Gurdon, J B

“…Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been…”
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Protocols for transgenesis at a safe harbor site in the Xenopus laevis genome using CRISPR-Cas9 by Shibata, Yuki, Okumura, Akinori, Mochii, Makoto, Suzuki, Ken-ichi T.

“…We have established a new transgenesis protocol based on CRISPR-Cas9, “New and Easy XenopusTransgenesis (NEXTrans),” and identified a novel safe harbor site in…”
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In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration by Suzuki, Miyuki, Takagi, Chiyo, Miura, Shinichirou, Sakane, Yuto, Suzuki, Makoto, Sakuma, Tetsushi, Sakamoto, Naoaki, Endo, Tetsuya, Kamei, Yasuhiro, Sato, Yuko, Kimura, Hiroshi, Yamamoto, Takashi, Ueno, Naoto, Suzuki, Ken‐ichi T.

“…Xenopus laevis tadpoles can completely regenerate their appendages, such as tail and limbs, and therefore provide a unique model to decipher the molecular…”
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Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration by Satoh, Akira, Mitogawa, Kazumasa, Saito, Nanami, Suzuki, Miyuki, Suzuki, Ken-ichi T., Ochi, Haruki, Makanae, Aki

“…Limb regeneration is considered a form of limb redevelopment because of the molecular and morphological similarities. Forming a regeneration blastema is, in…”
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