Antimicrobial potential of Actinomycetes species isolated from marine environment

Objective:To evaluate the antimicrobial activity of Actinomycetes species isolated from marine environment.Methods:Twenty one strains of Actinomyceteswere isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak meth...

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Published in:	Asian Pacific journal of tropical biomedicine Vol. 2; no. 6; pp. 469 - 473
Main Authors:	Valli, S, Suvathi, Sugasini S, Aysha, OS, Nirmala, P, Vinoth, Kumar P, Reena, A
Format:	Journal Article
Language:	English
Published:	China Elsevier B.V 01-06-2012 Asian Pacific Tropical Medicine Press
Subjects:	16S 16S rRNA Actinobacteria - chemistry Actinobacteria - classification Actinobacteria - isolation & purification Actinobacteria - physiology activity Anti-Infective Agents - isolation & purification Antibacterial Antibacterial substance Antibiosis Antimicrobial Antimicrobial activity Aquatic Organisms - chemistry Aquatic Organisms - classification Aquatic Organisms - isolation & purification Aquatic Organisms - physiology Bacillus subtilis Brackish character Cluster Analysis Cross-streak Cross-streak method diffusion disc DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects identification Kirby-Bauer Kirby-Bauer disc diffusion method Marine Actinomycetes MarineActinomycetes method Molecular Molecular identification Morphological Morphological character Original Phylogeny Pseudomonas RNA, Ribosomal, 16S - genetics rRNA Sequence Analysis, DNA Staphylococcus Streptomyces substance Vibrio harveyi ISW, India, Tamil Nadu, Chennai Dist., Adyar Estuary ISW, India, Tamil Nadu, Muttukadu 16S rRNA Antimicrobial activity Marine Actinomycetes Molecular identification Streptomyces Cross-streak method Morphological character Kirby-Bauer disc diffusion method Antibacterial substance
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Summary:	Objective:To evaluate the antimicrobial activity of Actinomycetes species isolated from marine environment.Methods:Twenty one strains of Actinomyceteswere isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak method against two gram-positive and eight gram-negative bacteria.The most potent strains C11 and C12 were selected from which antibacterial substances were extracted. The antibacterial activities of the extracts were performed using Kirby-Bauer disc diffusion method. Molecular identification of those isolates was done.Results:All those twenty one isolates were active against at least one of the test organisms. Morphological characters were recorded. C11 showed activity againstStaphylococcusspecies(13.0暲0.5 mm),Vibrio harveyi(11.0暲0.2 mm),Pseudomonas species(12.0暲0.3 mm). C12 showed activity against Staphylococcus species(16.0暲0.4 mm),Bacillus subtilis(11.0暲0.2 mm),Vibrio harveyi(9.0暲0.1 mm),Pseudomonas species(10.0暲0.2 mm). 16S rRNA pattern strongly suggested that C11 and C12 strains were Streptomyces species.Conclusions:The results of the present investigation reveal that the marineActinomycetesfrom coastal environment are the potent source of novel antibiotics.Isolation, characterization and study of Actinom ycetescan be useful in discovery of novel species of Actinomy cetes.
Bibliography:	Valli S;Suvathi Sugasini S;Aysha OS;Nirmala P;Vinoth Kumar P;Reena A;PG and Research Department of Microbiology, Mohamed Sathak College of Arts & Science ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2221-1691 2588-9222
DOI:	10.1016/S2221-1691(12)60078-1