High doses of clethodim-based herbicide GrassOut Max poses reproductive hazard by affecting male reproductive function and early embryogenesis in Swiss albino mice

High doses of clethodim-based herbicide GrassOut Max poses reproductive hazard by affecting male reproductive function and early embryogenesis in Swiss albino mice

Clethodim is a widely used and approved class II herbicide, with little information about its impact on the reproductive system. Herein, we investigated the male reproductive toxicity of clethodim using a mouse model. GrassOut Max (26% clethodim-equivalent) or analytical grade clethodim (≥90%) were...

Full description

Saved in:
Bibliographic Details
Published in:Chemosphere (Oxford) Vol. 336; p. 139215
Main Authors: Dcunha, Reyon, Kumari, Sandhya, Najar, Mohd Altaf, Aravind, Anjana, Suvarna, Keerthana Sandesh, Hanumappa, Ananda, Mutalik, Sadhana P., Mutalik, Srinivas, Kalthur, Sneha Guruprasad, Rajanikant, G.K., Siddiqui, Sazada, Alrumman, Sulaiman, Alamri, Saad Abdurahamn M., Raghu, Shamprasad Varija, Adiga, Satish Kumar, Kannan, Nagarajan, Thottethodi Subrahmanya, Keshava Prasad, Kalthur, Guruprasad
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-09-2023
Subjects:
Animals
Clethodim
Embryonic Development
Endocrine disruptor
Female
Herbicide
Herbicides - metabolism
Herbicides - toxicity
Male
Mice
Pregnancy
Proteomics
Semen
Sperm motility
Spermatozoa - metabolism
Testis - metabolism
Testosterone
Testosterone
Endocrine disruptor
Sperm motility
Clethodim
Herbicide
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Clethodim is a widely used and approved class II herbicide, with little information about its impact on the reproductive system. Herein, we investigated the male reproductive toxicity of clethodim using a mouse model. GrassOut Max (26% clethodim-equivalent) or analytical grade clethodim (≥90%) were given orally to male mice for 10 d in varying doses. All parameters were assessed at 35 d post-treatment. Significant decrease in testicular weight, decreased germ cell population, elevated DNA damage in testicular cells and lower serum testosterone level was observed post clethodim based herbicide exposure. Epididymal spermatozoa were characterized with significant decrease in motility, elevated DNA damage, abnormal morphology, chromatin immaturity and, decreased acetylated-lysine of sperm proteins. In the testicular cells of clethodim-based herbicide treated mice, the expression of Erβ and Gper was significantly higher. Proteomic analysis revealed lower metabolic activity, poor sperm-oocyte binding potential and defective mitochondrial electron transport in spermatozoa of clethodim-based herbicide treated mice. Further, fertilizing ability of spermatozoa was compromised and resulted in defective preimplantation embryo development. Together, our data suggest that clethodim exposure risks male reproductive function and early embryogenesis in Swiss albino mice via endocrine disrupting function. [Display omitted] •Clethodim affects spermatogenesis and produces poor quality sperm.•Clethodim acts as a potential endocrine disruptor.•Decreased fertilization potential of spermatozoa observed post clethodim exposure.•Paternal exposure to clethodim leads to defective embryo development.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2023.139215