CD8+T cells Recognize an Inclusion Membrane-Associated Protein from the Vacuolar Pathogen Chlamydia trachomatis

During infection with Chlamydia trachomatis, CD8+T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8+T cells recognize antigens processed from cytosolic proteins, the Chlamydia antigens recognized by these CD8+T cells...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 98; no. 3; pp. 1160 - 1165
Main Authors:	Fling, Steven P., Sutherland, R. Alec, Steele, Lisa N., Hess, Bruce, Sarah E. F. D'Orazio, Maisonneuve, Jean-François, Lampe, Mary F., Probst, Peter, Starnbach, Michael N.
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 30-01-2001 National Acad Sciences The National Academy of Sciences
Subjects:	Animals Antigens Bacterial Proteins - genetics Bacterial Proteins - immunology Base Sequence Biological Sciences CD8-Positive T-Lymphocytes - immunology Cell Line Cell lines Cells Cells, Cultured Chlamydia Chlamydia trachomatis - genetics Chlamydia trachomatis - immunology Chlamydia trachomatis - pathogenicity DNA Female Gene Library Immunohistochemistry Immunology Inclusion bodies Infections Membrane Proteins - genetics Membrane Proteins - immunology Membranes Mice Mice, Inbred BALB C Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Proteins Restriction Mapping T lymphocytes Vaccination Vacuoles - microbiology
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Summary:	During infection with Chlamydia trachomatis, CD8+T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8+T cells recognize antigens processed from cytosolic proteins, the Chlamydia antigens recognized by these CD8+T cells very likely have access to the host cell cytoplasm during infection. The identity of these C. trachomatis proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from C. trachomatis recognized by protective CD8+T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human C. trachomatis serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all C. trachomatis serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of C. trachomatis with its host cell.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Communicated by John J. Mekalanos, Harvard Medical School, Boston, MA To whom reprint requests should be addressed at: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, or Corixa Corporation, 1124 Columbia Street, Seattle, WA 98104. E-mail: starnbach@hms.harvard.edu or sfling@corixa.com.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.98.3.1160