Search Results - "Susanne Bohlken-Fascher"
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Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay
Published in Parasites & vectors (13-05-2016)“…Leishmania donovani (LD) is a protozoan parasite transmitted to humans from sand flies, which causes Visceral Leishmaniasis (VL). Currently, the diagnosis is…”
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Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source
Published in Foods (01-10-2020)“…Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and…”
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A Multi-Country, Single-Blinded, Phase 2 Study to Evaluate a Point-of-Need System for Rapid Detection of Leishmaniasis and Its Implementation in Endemic Settings
Published in Microorganisms (Basel) (12-03-2021)“…With the advancement of isothermal nucleic acid amplification techniques, detection of the pathogenic DNA in clinical samples at point-of-need is no longer a…”
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Suitcase Lab for Rapid Detection of SARS-CoV‑2 Based on Recombinase Polymerase Amplification Assay
Published in Analytical chemistry (Washington) (02-02-2021)“…In March 2020, the SARS-CoV-2 virus outbreak was declared as a world pandemic by the World Health Organization (WHO). The only measures for controlling the…”
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Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka
Published in Parasites & vectors (22-12-2018)“…Leishmaniasis is a disease caused by vector-borne protozoans. In Sri Lanka, the cutaneous form of the disease is predominant, which is usually diagnosed using…”
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Diagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysis
Published in Scientific reports (06-03-2019)“…Zika virus (ZIKV) is a mosquito-borne flavivirus. Homologous proteins of different flaviviruses display high degrees of sequence identity, especially within…”
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Molecular and Serological Footprints of Mycobacterium avium Subspecies Infections in Zoo Animals
Published in Veterinary sciences (23-08-2020)“…Background: Mycobacteria of the Mycobacterium avium complex (MAC) pose a significant risk to zoological collections. Mycobacterium avium subspecies…”
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Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus ducreyi in Yaws-Like Lesions
Published in Tropical medicine and infectious disease (06-10-2020)“…Yaws is a skin debilitating disease caused by subspecies with most cases reported in children. World Health Organization (WHO) aims at total eradication of…”
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Development of Rapid Extraction Method of Mycobacterium avium Subspecies paratuberculosis DNA from Bovine Stool Samples
Published in Diagnostics (Basel) (29-03-2019)“…The rapid identification of subspecies (MAP) infected animals within the herd is essential for preventing the spread of the disease as well as avoiding human…”
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Differentiation of Capripox Viruses by Nanopore Sequencing
Published in Vaccines (Basel) (06-04-2021)“…The genus capripoxvirus (CaPV), family , includes three virus species: goatpox virus (GPV), sheeppox virus (SPV) and lumpy skin disease virus (LSDV). CaPV…”
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Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay
Published in PLoS currents (25-01-2017)“…Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped…”
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Multiplex Recombinase polymerase amplification assay for simultaneous detection of 'Treponema pallidum' and 'Haemophilus ducreyi' in yaws-like lesions
Published in Tropical medicine and infectious disease (01-12-2020)“…Yaws is a skin debilitating disease caused by 'Treponema pallidum' subspecies 'pertenue' with most cases reported in children. World Health Organization (WHO)…”
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Recombinase polymerase amplification assays for the identification of pork and horsemeat
Published in Food chemistry (30-08-2020)“…•Pork and horse DNA can be detected in eleven minutes.•The assays are based on the technique recombinase polymerase amplification.•The whole procedure can be…”
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Recombinase polymerase amplification assay for rapid detection of Monkeypox virus
Published in Diagnostic microbiology and infectious disease (01-09-2019)“…In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA)…”
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The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress
Published in Cell reports (Cambridge) (27-08-2024)“…The tumor suppressor p53 and its antagonists MDM2 and MDM4 integrate stress signaling. For instance, dysbalanced assembly of ribosomes in nucleoli induces p53…”
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Development of a pan-rickettsial molecular diagnostic test based on recombinase polymerase amplification assay
Published in Analytical biochemistry (01-03-2018)“…Rickettsioses are zoonotic vector-transmitted bacterial infections leading to flu-like symptoms and can progress to severe illness in humans. The gold standard…”
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Serotyping of foot-and-mouth disease virus using oxford nanopore sequencing
Published in Journal of virological methods (01-01-2019)“…•The causative serotype of Foot and Mouth Disease Virus can be identified via nanopore sequencing within five hours.•The whole procedure was conducted in a…”
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Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology
Published in Methods in molecular biology (Clifton, N.J.) (2020)“…Development of novel point of care diagnostic methods in order to help in implementing disease control program and identifying the causative agent of an…”
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Combination random isothermal amplification and nanopore sequencing for rapid identification of the causative agent of an outbreak
Published in Journal of clinical virology (01-09-2018)“…•The causative agent of an outbreak can be identified by nanopore sequencing in 7 h.•The whole procedure was conducted in a mobile suitcase laboratory, which…”
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