Ammonia Accumulation as an Index of Glufosinate-Tolerant Soybean Cell Lines

Selection of soybean (Glycine max L. cv. SJ 4) cell lines tolerant to glufosinate was attempted using cell suspension cultures induced from hypocotyls of young seedlings. The cell suspension was cultured on MS basic medium supplemented with B5 vitamins, 0.3% sucrose, and 10 mg/L NAA. Using a stepwis...

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Bibliographic Details
Published in:	Pesticide biochemistry and physiology Vol. 68; no. 2; pp. 102 - 106
Main Authors:	Pornprom, Tosapon, Surawattananon, Srisom, Srinives, Peerasak
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-10-2000 Elsevier
Subjects:	Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions ammonia accumulation Biological and medical sciences Biotechnology cell suspension culture Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants glufosinate herbicide-tolerant soybean Varietal selection. Specialized plant breeding, plant breeding aims cell suspension culture glufosinate ammonia accumulation herbicide-tolerant soybean Biotechnology Cell culture Organic phosphinate Artificial selection Index GLUFOSINATE Suspension culture Hypocotyl Dicotyledones Angiospermae Selection criterion Pesticide resistance Pesticides Tolerance Experimental study Glycine max Herbicide Oil plant(vegetal) Indicator Grain legume Ammonia Cell line Leguminosae Aminoacid Genetic improvement Spermatophyta Biological accumulation
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Summary:	Selection of soybean (Glycine max L. cv. SJ 4) cell lines tolerant to glufosinate was attempted using cell suspension cultures induced from hypocotyls of young seedlings. The cell suspension was cultured on MS basic medium supplemented with B5 vitamins, 0.3% sucrose, and 10 mg/L NAA. Using a stepwise selection with increasing concentration of herbicide, a soybean line tolerant to 10−7 M glufosinate was obtained through direct selection of diploid cells in the suspension culture. It is referred to as 10−7 M glufosinate-tolerant soybean cell line. Determination of ammonia concentration in the herbicide-treated cells indicated that the normal (nontolerant) cells accumulated up to 15-fold more ammonia than the tolerant cells. This information suggested that lower ammonia accumulation in the tolerant cells can be used as an indicator to select for glufosinate-tolerant soybean cell lines.
ISSN:	0048-3575 1095-9939
DOI:	10.1006/pest.2000.2502