Analysis of varicella-zoster virus and herpes simplex virus in various clinical samples by the use of different PCR assays

Rapid and reliable detection of varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and -2 (HSV-2) is of clinical significance in immunocompromised patients and patients with infections of the central nervous system. This paper describes the detection of VZV and HSV using the commer...

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Published in:Journal of virological methods Vol. 160; no. 1; pp. 193 - 196
Main Authors: Finnström, Niklas, Bergsten, Karin, Ström, Helena, Sundell, Therese, Martin, Sophie, Wutzler, Peter, Sauerbrei, Andreas
Format: Journal Article
Language:English
Published: Kidlington Elsevier B.V 01-09-2009
Elsevier
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Summary:Rapid and reliable detection of varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and -2 (HSV-2) is of clinical significance in immunocompromised patients and patients with infections of the central nervous system. This paper describes the detection of VZV and HSV using the commercially available Affigene ® VZV and Affigene ® HSV 1/2 tracer kits in comparison to “in-house” polymerase chain reaction (PCR) assays. For sample preparation, Qiagen (Hilden, Germany) and Affigene ® (Cepheid AB, Bromma, Sweden) DNA extraction kits were used. 175 samples were analyzed for VZV and 352 samples for HSV-1 and -2. Generally more positive results were obtained using the Affigene ® assays compared to the “in-house” methods independent of the DNA preparation method used. There were significant differences in sensitivity between the Affigene ® HSV 1/2 tracer and the “in-house” PCR assays for the detection of both HSV-1 and -2 in cerebrospinal fluid and vesicle/skin swabs. The Affigene ® HSV 1/2 and VZV tracers are very sensitive assays for detection of VZV and HSV. A wide variety of clinical samples can be examined in combination with either the Qiagen or the Affigene ® DNA extraction kits for preparation.
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ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2009.04.035