Glomerular and proximal tubular morphology after single nephron obstruction

Glomerular and proximal tubular morphology after single nephron obstruction

Glomerular and proximal tubular morphology after single nephron obstruction. This study examined the effect of blocking proximal tubule lumens on glomerular and early proximal tubular morphology. Single nephrons in the rat kidney were blocked with wax by micropuncture. After one day, one week, or on...

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Bibliographic Details
Published in:Kidney international Vol. 36; no. 6; pp. 1050 - 1060
Main Authors: Tanner, George A., Evan, Andrew P., Summerlin, Patricia B., Knopp, Lisa C.
Format: Journal Article
Language:English
Published: New York, NY Elsevier Inc 01-12-1989
Nature Publishing
Subjects:
Animals
Atrophy
Biological and medical sciences
Kidney Glomerulus - pathology
Kidney Tubules, Proximal - pathology
Male
Medical sciences
Microscopy, Electron
Microvilli - ultrastructure
Nephrology. Urinary tract diseases
Nephrons - pathology
Rats
Time Factors
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
Renal glomerulus
Pathology
Animal model
Vertebrata
Mammalia
Urinary system disease
Rat
Ultrastructure
Animal
Rodentia
Nephron
Obstruction
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Summary:Glomerular and proximal tubular morphology after single nephron obstruction. This study examined the effect of blocking proximal tubule lumens on glomerular and early proximal tubular morphology. Single nephrons in the rat kidney were blocked with wax by micropuncture. After one day, one week, or one month of obstruction, the kidneys were fixed with glutaraldehyde by intravascular perfusion, and nephron structure was examined by light and electron microscopy. Following obstruction, glomerular changes developed more slowly than tubular changes. After one day, the only change noted in some glomeruli was the presence of inflammatory cells. The only tubule change upstream to the block was a focal loss of apical microvilli. This is in contrast to the severe, damage previously reported (Evan, Tanner: Kidney Int 30: 818–827, 1986) in downstream proximal tubule segments at this time. After one month of obstruction, glomerular size was decreased and the glomerular filtration membrane was abnormal. Tubular cell size was decreased, apical microvilli were lost, basolateral interdigitations were reduced, and mitochondria were fewer and abnormally oriented. Interstitial fibrosis was present. Changes in nephron structure develop slowly after obstruction, perhaps because continued filtration and reabsorption maintain nephron integrity. Eventually, blocked nephrons atrophy, probably because of reduced blood flow, disuse, and inflammatory responses.
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ISSN:0085-2538
1523-1755
DOI:10.1038/ki.1989.300