Gingival fibroblasts grown from cyclosporin-treated patients show a reduced production of matrix metalloproteinase-1 (MMP-1) compared with normal gingival fibroblasts, and cyclosporin down-regulates the production of MMP-1 stimulated by pro-inflammatory cytokines

Background and Objective: Cyclosporin‐induced gingival overgrowth arises from an alteration in collagen homeostasis and is enhanced by inflammatory changes in the gingival tissues. The aim of this study was to investigate the interaction among interleukin‐1, oncostatin M, cyclosporin and nifedipine...

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Published in:	Journal of periodontal research Vol. 42; no. 6; pp. 580 - 588
Main Authors:	Sukkar, T. Z., Thomason, J. M., Cawston, T. E., Lakey, R., Jones, D., Catterall, J., Seymour, R. A.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-12-2007 Blackwell
Subjects:	Biological and medical sciences Calcium Channel Blockers - pharmacology Case-Control Studies Cells, Cultured collagen cyclosporin Cyclosporine - adverse effects Cyclosporine - pharmacology cytokine Down-Regulation Fibroblasts - metabolism Gingiva - cytology Gingiva - metabolism gingival fibroblast Gingival Overgrowth - chemically induced Gingival Overgrowth - metabolism Humans Immunosuppressive Agents - adverse effects Interleukin-1 - physiology matrix metalloproteinase Matrix Metalloproteinase 1 - biosynthesis Medical sciences Nifedipine - pharmacology Oncostatin M - physiology Otorhinolaryngology. Stomatology tissue inhibitor of metalloproteinase Tissue Inhibitor of Metalloproteinase-1 - biosynthesis Human Enzyme Stomatology Cytokine Metalloendopeptidases Inflammation gingival fibroblast Peptidases Tissue Regulation(control) Treatment Collagen tissue inhibitor of metalloproteinase Hydrolases cyclosporin matrix metalloproteinase Ciclosporin Immunosuppressive agent Gingiva Fibroblast Comparative study Interstitial collagenase
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Summary:	Background and Objective: Cyclosporin‐induced gingival overgrowth arises from an alteration in collagen homeostasis and is enhanced by inflammatory changes in the gingival tissues. The aim of this study was to investigate the interaction among interleukin‐1, oncostatin M, cyclosporin and nifedipine in promoting the up‐regulation of matrix metalloproteinase‐1 (MMP‐1) and tissue inhibitor of metalloproteinase by gingival fibroblasts. Material and Methods: Fibroblast cultures (n = 5) were obtained from healthy controls and from patients with cyclosporin‐induced gingival overgrowth, and cells were harvested between the fourth and ninth passages. Cells were stimulated with interleukin‐1 and oncostatin M, alone or in combination, and with different concentrations of cyclosporin (0–2000 ng/mL) and nifedipine (0–200 ng/mL). MMP‐1 and tissue inhibitor of metalloproteinase‐1 production was determined using an enzyme‐linked immunosorbent assay technique. A CyQuant cell proliferation assay was used to determine the DNA concentration in the sample. Results: Fibroblasts obtained from patients with cyclosporin‐induced gingival overgrowth produced significantly lower levels of MMP‐1 than control fibroblasts (p < 0.001); tissue inhibitor of metalloproteinase‐1 levels were significantly lower (p < 0.05), and the ratio of MMP‐1 to tissue inhibitor of metalloproteinase‐1 was reduced, in the conditioned medium of patients with cyclosporin‐induced gingival overgrowth compared with controls. Interleukin‐1 and oncostatin M produced a significant increase in the up‐regulation of MMP‐1, which was reversed when cyclosporin and nifedipine were added to the cell cultures (p < 0.05). Conclusion: Pro‐inflammatory cytokines significantly up‐regulate MMP‐1 in cultured gingival fibroblasts. Up‐regulation is attenuated by both cyclosporin and nifedipine. The interaction may account for the synergism between inflammation and cyclosporin‐induced gingival overgrowth.
Bibliography:	ArticleID:JRE986 istex:D3F94328AE43BB22600EC904F4AD91428FE71545 ark:/67375/WNG-VP5VKQX5-V ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3484 1600-0765
DOI:	10.1111/j.1600-0765.2007.00986.x