Utilization of UPLC-PDA and GC–MS/MS coupled with metabolomics analysis to identify bioactive metabolites in medicinal turmeric at different ages for the quality assurance

Utilization of UPLC-PDA and GC–MS/MS coupled with metabolomics analysis to identify bioactive metabolites in medicinal turmeric at different ages for the quality assurance

•Integration of holistic approach of traditional medicine into the quality control of herbal medicine.•New insight in quality control of turmeric used for traditional medicine production.•Other active compounds, namely Ar-turmerone, turmerone, curlone and zingiberene, should be used as quality marke...

Full description

Saved in:
Bibliographic Details
Published in:Phytomedicine (Stuttgart) Vol. 102; p. 154157
Main Authors: Pantharos, Patchaya, Sukcharoen, Panisara, Phadungrakwittaya, Rattana, Akarasereenont, Pravit, Booranasubkajorn, Suksalin, Lumlerdkij, Natchagorn
Format: Journal Article
Language:English
Published: Germany Elsevier GmbH 20-07-2022
Subjects:
Chemical Profiles
Curcuma - chemistry
Curcumin - pharmacology
Curcumin content
Gas Chromatography-Mass Spectrometry
Harvesting time
Metabolomics
Tandem Mass Spectrometry
Traditional Medicine
Turmeric rhizome
GC–MS/MS
LOD
R2
Curcumin content
RSD
PTFE
Traditional Medicine
HCA
BEH column
Harvesting time
Chemical Profiles
AR grade
LOQ
PCA
SD
NIST
X
Turmeric rhizome
HPLC
UPLC-PDA
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Integration of holistic approach of traditional medicine into the quality control of herbal medicine.•New insight in quality control of turmeric used for traditional medicine production.•Other active compounds, namely Ar-turmerone, turmerone, curlone and zingiberene, should be used as quality markers in addition to the conventional marker curcumin.•We provide evidence that suggest an optimal harvest time for medicinal turmeric. Available monographs often suggest measurement of curcumin or curcuminoids for quality control of turmeric-based medicines/products. However, one compound is not enough to indicate the quality of traditional herbal medicines due to the holistic approach. Furthermore, to ensure high quality of such products, good harvesting practice plays an important role in the quality control of turmeric raw materials. This study aimed to indicate quality markers of turmeric and to suggest optimum harvesting times for turmeric used for medicinal purposes by integration of Thai traditional medicine knowledge. Turmeric rhizomes at 4, 6, 8, 9, 10, and 11 months old were analyzed. UPLC-PDA was used for quantitation of curcumin. GC–MS/MS was used to obtain chemical profiles of turmeric volatile oil. PCA, Volcano plot, and HCA were performed to identify similarities or differences of the data. Turmeric aged between 6 and 10 months old contained > 5%w/w of curcumin, which complied with Thai Herbal Pharmacopoeia 2021. GC–MS/MS analysis suggested suitable chemical markers, namely Ar-turmerone, turmerone, curlone, and zingiberene, because they exhibited pharmacological activities related to the traditional uses of turmeric. Eucalyptol, santalene, β-caryophyllene, cis-β-farnesene, α-caryophyllene, curcumene, β-bisabolene, β-sesquiphellandrene, and cis-sesquisabinene hydrate were also tentatively identified. Later, the multivariate analysis revealed that turmeric aged between 6 and 10 months old showed similar metabolite profiles. Based on curcumin content and chemical profiles, turmeric aged between 6 and 10 months old are appropriate for uses in Thai traditional medicine. Our study suggests additional information in the quality control of turmeric to assure its efficacy, especially for uses in traditional medicines. [Display omitted]
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0944-7113
1618-095X
DOI:10.1016/j.phymed.2022.154157