Search Results - "Sugiura, Yukio"

The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications by Negi, Shigeru, Imanishi, Miki, Hamori, Mami, Kawahara-Nakagawa, Yuka, Nomura, Wataru, Kishi, Kanae, Shibata, Nobuhito, Sugiura, Yukio

“…Zinc finger proteins are abundant in the human proteome and are responsible for a variety of functions. The domains that constitute zinc finger proteins are…”
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Cytochrome c polymerization by successive domain swapping at the C-terminal helix by Hirota, Shun, Hattori, Yoko, Nagao, Satoshi, Taketa, Midori, Komori, Hirofumi, Kamikubo, Hironari, Wang, Zhonghua, Takahashi, Isao, Negi, Shigeru, Sugiura, Yukio, Kataoka, Mikio, Higuchi, Yoshiki, Toyoshima, Chikashi

“…Cytochrome c (cyt c) is a stable protein that functions in a monomeric state as an electron donor for cytochrome c oxidase. It is also released to the cytosol…”
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Effect of Charged-cluster Distribution of the ZIF268 Zinc Finger Surface on Cell Membrane Permeability by Negi, Shigeru, Hamori, Mami, Kawahara-Nakagawa, Yuka, Mashimo, Masato, Imanishi, Miki, Kuki, Nozomi, Kawahito, Yuri, Manabe, Takayuki, Kawamura, Nobuyuki, Yasukawa, Jyunichiro, Kitagishi, Hiroaki, Sugiura, Yukio

“…To investigate the importance of charged-cluster distribution in cell permeability, ZIF268 zinc finger (ZF) was examined as a new target protein. We found that…”
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Transpeptidation Reaction Mediated by Ligand- and Metal Cofactor-Substituted Sortase A from Staphylococcus aureus by Negi, Shigeru, Hamori, Mami, Sato, Ayaka, Shimizu, Kyoko, Kawahara-Nakagawa, Yuka, Manabe, Takayuki, Shibata, Nobuhito, Kitagishi, Hiroaki, Mashimo, Masato, Sugiura, Yukio

“…Sortase A is used for the post-translational modification of proteins in vitro and in cell, and it is known that amino acid residues involved in Ca2+ binding…”
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New Redesigned Zinc-Finger Proteins: Design Strategy and Its Application by Negi, Shigeru, Imanishi, Miki, Matsumoto, Makoto, Sugiura, Yukio

“…The design of DNA‐binding proteins for the specific control of the gene expression is one of the big challenges for several research laboratories in the…”
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Cellular Uptake of Arginine-Rich Peptides: Roles for Macropinocytosis and Actin Rearrangement by Nakase, Ikuhiko, Niwa, Miki, Takeuchi, Toshihide, Sonomura, Kazuhiro, Kawabata, Noriko, Koike, Yukihiro, Takehashi, Masanori, Tanaka, Seigo, Ueda, Kunihiro, Simpson, Jeremy C, Jones, Arwyn T, Sugiura, Yukio, Futaki, Shiroh

“…The use of membrane-permeable peptides as carrier vectors for the intracellular delivery of various proteins and macromolecules for modifying cellular function…”
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Direct and Rapid Cytosolic Delivery Using Cell-Penetrating Peptides Mediated by Pyrenebutyrate by Takeuchi, Toshihide, Kosuge, Michie, Tadokoro, Akiko, Sugiura, Yukio, Nishi, Mayumi, Kawata, Mitsuhiro, Sakai, Naomi, Matile, Stefan, Futaki, Shiroh

“…Intracellular delivery of bioactive molecules using arginine-rich peptides, including oligoarginine and HIV-1 Tat peptides, is a recently developed technology…”
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Possible Existence of Common Internalization Mechanisms among Arginine-rich Peptides by Suzuki, Tomoki, Futaki, Shiroh, Niwa, Miki, Tanaka, Seigo, Ueda, Kunihiro, Sugiura, Yukio

“…Basic peptides such as human immunodeficiency virus type 1 (HIV-1) Tat-(48–60) and DrosophilaAntennapedia-(43–58) have been reported to have a membrane…”
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Importance of two-dimensional cation clusters induced by protein folding in intrinsic intracellular membrane permeability by Negi, Shigeru, Hamori, Mami, Kawahara-Nakagawa, Yuka, Imanishi, Miki, Kurehara, Miku, Kitada, Chieri, Kawahito, Yuri, Kishi, Kanae, Manabe, Takayuki, Kawamura, Nobuyuki, Kitagishi, Hiroaki, Mashimo, Masato, Shibata, Nobuhito, Sugiura, Yukio

“…We investigated the cell penetration of Sp1 zinc finger proteins (Sp1 ZF) and the mechanism via which the total cationic charge and distribution of cationic…”
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Efficient cleavage of DNA oligonucleotides by a non-FokI-type zinc finger nuclease containing one His4-type finger domain derived from the first finger domain of Sp1 by Negi, Shigeru, Yoshioka, Michiko, Mima, Hiroko, Mastumoto, Makoto, Suzuki, Michiko, Yokoyama, Mao, Kano, Koji, Sugiura, Yukio

“…[Display omitted] In this study, we sought to improve the hydrolytic activity of a His4-type single finger domain (f2), which was previously derived from the…”
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Translocation of Branched-Chain Arginine Peptides through Cell Membranes:  Flexibility in the Spatial Disposition of Positive Charges in Membrane-Permeable Peptides by Futaki, Shiroh, Nakase, Ikuhiko, Suzuki, Tomoki, Zhang, Sugiura, Yukio

“…A basic peptide derived from HIV-1 Tat has been reported to have the ability to translocate through cell membranes and to bring exogenous proteins into cells…”
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An Arginine Residue Instead of a Conserved Leucine Residue in the Recognition Helix of the Finger 3 of Zif268 Stabilizes the Domain Structure and Mediates DNA Binding by Negi, Shigeru, Imanishi, Miki, Sasaki, Maeko, Tatsutani, Kazuya, Futaki, Shiroh, Sugiura, Yukio

“…The Cys2His2-type zinc finger is a common DNA binding motif that is widely used in the design of artificial zinc finger proteins. In almost all Cys2His2-type…”
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Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by an Artificial Multi-Zinc Finger Protein by Morisaki, Tatsuya, Imanishi, Miki, Futaki, Shiroh, Sugiura, Yukio

“…Artificial transcription factors targeting any desired genes are very attractive but require specific DNA binding domains in order to address a single site for…”
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Contribution of Individual Zinc Ligands to Metal Binding and Peptide Folding of Zinc Finger Peptides by Nomura, Akiko, Sugiura, Yukio

“…Little is known about the contribution of individual zinc-ligating amino acid residues for coupling between zinc binding and protein folding in zinc finger…”
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NMR Structure of Transcription Factor Sp1 DNA Binding Domain by Oka, Shinichiro, Shiraishi, Yasuhisa, Yoshida, Takuya, Ohkubo, Tadayasu, Sugiura, Yukio, Kobayashi, Yuji

“…To understand the DNA recognition mechanism of zinc finger motifs of transcription factor Sp1, we have determined the solution structure of DNA-binding domain…”
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Rational design of DNA sequence-specific zinc fingers by Kono, Hidetoshi, Imanishi, Miki, Negi, Shigeru, Tatsutani, Kazuya, Sakaeda, Yui, Hashimoto, Ayaka, Nakayama, Chie, Futaki, Shiroh, Sugiura, Yukio

“…► A rational scheme for computationally designing DNA binding proteins is proposed. ► The structure and binding of the designed proteins were experimentally…”
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Zinc finger–zinc finger interaction between the transcription factors, GATA-1 and Sp1 by Imanishi, Miki, Imamura, Chika, Higashi, Chika, Yan, Wei, Negi, Shigeru, Futaki, Shiroh, Sugiura, Yukio

“…► The zinc fingers of the transcription factors, Sp1 and GATA-1, interact with each other. ► The zinc finger–zinc finger interaction is an entropically driven…”
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Acid wash in determining cellular uptake of Fab/cell-permeating peptide conjugates by Kameyama, Shouju, Horie, Mayo, Kikuchi, Takeo, Omura, Takao, Tadokoro, Akiko, Takeuchi, Toshihide, Nakase, Ikuhiko, Sugiura, Yukio, Futaki, Shiroh

“…Successful intracellular delivery of various bioactive molecules has been reported using cell‐permeating peptides (CPPs) as delivery vectors. To determine the…”
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Membrane permeability commonly shared among arginine-rich peptides by Futaki, Shiroh, Goto, Susumu, Sugiura, Yukio

“…Delivery of proteins and other macromolecules using membrane‐permeable carrier peptides is a recently developed novel technology, which enables us to modulate…”
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Metal-Stimulated Regulation of Transcription by an Artificial Zinc-Finger Protein by Imanishi, Miki, Nakaya, Tomohiro, Morisaki, Tatsuya, Noshiro, Daisuke, Futaki, Shiroh, Sugiura, Yukio

“…Transcriptional switch: A zinc‐finger protein (CDH2‐ZF3) has been created in which a Cys was replaced by an Asp. The ZnII binding affinity of the CDH2‐type…”
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