In vivo magnetic resonance imaging of atherosclerotic lesions with a newly developed Evans blue-DTPA-gadolinium contrast medium in apolipoprotein-E-deficient mice

Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with en...

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Bibliographic Details
Published in:	Journal of vascular research Vol. 45; no. 2; p. 123
Main Authors:	Yasuda, Satoshi, Ikuta, Kenjiro, Uwatoku, Toyokazu, Oi, Keiji, Abe, Kohtaro, Hyodo, Fuminori, Yoshimitsu, Kengo, Sugimura, Kohtaro, Utsumi, Hideo, Katayama, Yoshiki, Shimokawa, Hiroaki
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-2008
Subjects:	Animals Aorta - metabolism Aorta - pathology Apolipoproteins E - deficiency Apolipoproteins E - genetics Apolipoproteins E - metabolism Atherosclerosis - genetics Atherosclerosis - metabolism Atherosclerosis - pathology Contrast Media - administration & dosage Disease Models, Animal Injections, Intravenous Magnetic Resonance Angiography - methods Male Mice Mice, Inbred C57BL Mice, Knockout Organometallic Compounds - administration & dosage Pentetic Acid - administration & dosage Pentetic Acid - analogs & derivatives Time Factors
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Summary:	Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. We used male apolipoprotein-E-deficient (ApoE-/-) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 microM/kg body weight), MRI T(1) signal was obtained in vivo. Increased signal intensity in the aortic wall was noted within 10-20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE-/- mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.
ISSN:	1423-0135
DOI:	10.1159/000109930