Search Results - "Suen, Sylvia W.F."

CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank by Geurts, Maarten H., de Poel, Eyleen, Amatngalim, Gimano D., Oka, Rurika, Meijers, Fleur M., Kruisselbrink, Evelien, van Mourik, Peter, Berkers, Gitte, de Winter-de Groot, Karin M., Michel, Sabine, Muilwijk, Danya, Aalbers, Bente L., Mullenders, Jasper, Boj, Sylvia F., Suen, Sylvia W.F., Brunsveld, Jesse E., Janssens, Hettie M., Mall, Marcus A., Graeber, Simon Y., van Boxtel, Ruben, van der Ent, Cornelis K., Beekman, Jeffrey M., Clevers, Hans

“…Adenine base editing (ABE) enables enzymatic conversion from A-T into G-C base pairs. ABE holds promise for clinical application, as it does not depend on the…”
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Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids by Vonk, Annelotte M., van Mourik, Peter, Ramalho, Anabela S., Silva, Iris A.L., Statia, Marvin, Kruisselbrink, Evelien, Suen, Sylvia W.F., Dekkers, Johanna F., Vleggaar, Frank P., Houwen, Roderick H.J., Mullenders, Jasper, Boj, Sylvia F., Vries, Robert, Amaral, Margarida D., de Boeck, Kris, van der Ent, Cornelis K., Beekman, Jeffrey M.

“…This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In…”
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High-throughput functional assay in cystic fibrosis patient-derived organoids allows drug repurposing by Spelier, Sacha, de Poel, Eyleen, Ithakisiou, Georgia N, Suen, Sylvia W F, Hagemeijer, Marne C, Muilwijk, Danya, Vonk, Annelotte M, Brunsveld, Jesse E, Kruisselbrink, Evelien, van der Ent, Cornelis K, Beekman, Jeffrey M

“…Cystic fibrosis (CF) is a rare hereditary disease caused by mutations in the cystic fibrosis transmembrane conductance regulator ( ) gene. Recent therapies…”
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Forskolin-induced organoid swelling is associated with long-term cystic fibrosis disease progression by Muilwijk, Danya, de Poel, Eyleen, van Mourik, Peter, Suen, Sylvia W.F., Vonk, Annelotte M., Brunsveld, Jesse E., Kruisselbrink, Evelien, Oppelaar, Hugo, Hagemeijer, Marne C., Berkers, Gitte, de Winter-de Groot, Karin M., Heida-Michel, Sabine, Jans, Stephan R., van Panhuis, Hannah, van der Eerden, Menno M., van der Meer, Renske, Roukema, Jolt, Dompeling, Edward, Weersink, Els J.M., Koppelman, Gerard H., Vries, Robert, Zomer-van Ommen, Domenique D., Eijkemans, Marinus J.C., van der Ent, Cornelis K., Beekman, Jeffrey M.

“…Rationale Cystic fibrosis (CF) is a monogenic life-shortening disease associated with highly variable individual disease progression which is difficult to…”
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Repeatability and reproducibility of the Forskolin-induced swelling (FIS) assay on intestinal organoids from people with Cystic Fibrosis by Bierlaagh, Marlou C., Ramalho, Anabela S., Silva, Iris A.L., Vonk, Annelotte M., van den Bor, Rutger M., van Mourik, Peter, Pott, Johanna, Suen, Sylvia W.F., Boj, Sylvia F., Vries, Robert G.J., Lammertyn, Elise, Vermeulen, François, Amaral, Margarida D., de Boeck, Kris, van der Ent, Cornelis K., Beekman, Jeffrey M.

“…•An ECFS-endorsed SOP was used to measure CFTR function using forskolin-induced swelling.•The Forskolin-induced swelling (FIS) assay demonstrates high…”
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