Distribution and functional analysis of the two types of 8-vinyl reductase involved in chlorophyll biosynthesis in marine cyanobacteria

In the chlorophyll biosynthesis pathway, the 8-vinyl group of the chlorophyll precursor is reduced to an ethyl group by 8-vinyl reductase. Two isozymes of 8-vinyl reductase have been described in oxygenic photosynthetic organisms: one encoded by BciA and another by BciB . Only BciB contains an [Fe-S...

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Bibliographic Details
Published in:	Archives of microbiology Vol. 203; no. 6; pp. 3565 - 3575
Main Authors:	Suehiro, Haruka, Tanaka, Ryouichi, Ito, Hisashi
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2021 Springer Nature B.V
Subjects:	Biochemistry Biomedical and Life Sciences Biosynthesis Biotechnology Cell Biology Chlorophyll Cyanobacteria Ecology Functional analysis Harbors Iron Isoenzymes Life Sciences Marine environment Microbial Ecology Microbiology Microorganisms Nickel Original Paper Photosynthesis Reductase Reductases Superoxide dismutase Synechococcus 8-Vinyl reductase Chlorophyll biosynthesis Low-iron environment Adaptation Acaryochloris Synechococcus
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Summary:	In the chlorophyll biosynthesis pathway, the 8-vinyl group of the chlorophyll precursor is reduced to an ethyl group by 8-vinyl reductase. Two isozymes of 8-vinyl reductase have been described in oxygenic photosynthetic organisms: one encoded by BciA and another by BciB . Only BciB contains an [Fe-S] cluster and most cyanobacteria harbor this form; whereas a few contain BciA . Given this disparity in distribution, cyanobacterial BciA has remained largely overlooked, which has limited understanding of chlorophyll biosynthesis in these microorganisms. Here, we reveal that cyanobacterial BciA encodes a functional 8-vinyl reductase, as evidenced by measuring the in vitro activity of recombinant Synechococcus and Acaryochloris BciA. Genomic comparison revealed that BciB had been replaced by BciA during evolution of the marine cyanobacterium Synechococcus , and coincided with replacement of Fe-superoxide dismutase (SOD) with Ni-SOD. These findings imply that the acquisition of BciA confers an adaptive advantage to cyanobacteria living in low-iron oceanic environments.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0302-8933 1432-072X
DOI:	10.1007/s00203-021-02348-w