Search Results - "Sueda, Shinji"

Antibody immobilization for immunosensing by Sueda, Shinji

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Enzyme-based protein-tagging systems for site-specific labeling of proteins in living cells by Sueda, Shinji

“…Abstract Various protein-labeling methods based on the specific interactions between genetically encoded tags and synthetic probes have been proposed to…”
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Stepwise Preparation of a Polymer Comprising Protein Building Blocks on a Solid Support for Immunosensing Platform by MIYAO, Hiroki, UEMURA, Utaro, SUEDA, Shinji

“…In immunosensing, immobilization of the antibody on the sensing platform significantly influences the performance of the sensor. Herein, we propose a novel…”
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Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein by NISHI, Sairi, YAMAMOTO, Chihiro, YONEDA, Sawako, SUEDA, Shinji

“…Labeling with fluorescent proteins is now widely exploited for elucidating the functions and roles of target proteins in living cells. Previously, we developed…”
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Fluorescence Imaging of Extracellular Potassium Ion Using Potassium Sensing Oligonucleotide by Sato, Shinobu, Ohzawa, Shinsuke, Sota, Kojiro, Sakamoto, Naoto, Udo, Ayano, Sueda, Shinji, Matsuda, Tomoki, Nagai, Takeharu, Takenaka, Shigeori

“…Potassium-sensing oligonucleotide, PSO, a conjugate of a quadruplex structure-forming oligonucleotide with a peptide incorporating a Förster Resonance Energy…”
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A luminescent affinity tag for proteins based on the terbium(III)-binding peptide by Sueda, Shinji, Tanaka, Shogo, Inoue, Sayomi, Komatsu, Hideyuki

“…Genetically encoded tags attached to proteins of interest are widely exploited for proteome analysis. Here, we present Tb3+-binding peptides (TBPs) which can…”
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An SH2 Domain-Based Tyrosine Kinase Assay Using Biotin Ligase Modified with a Terbium(III) Complex by SUEDA, Shinji, SHINBOKU, Yuki, KUSABA, Takeshi

“…Src homology 2 (SH2) domains are modules of approximately 100 amino acids and are known to bind phosphotyrosine-containing sequences with high affinity and…”
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An ATP detection system based on the enzyme reaction with biotin protein ligase by Sueda, Shinji, Fujii, Satoshi

“…Adenosine triphosphate (ATP) is the energy currency of all living organisms and can be used as an indicator for cell proliferation and cytotoxicity. In the…”
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A fluorescence-based binding assay for proteins using the cell surface as a sensing platform by Hirano, Kazuki, Sueda, Shinji

“…Protein–protein interaction (PPI) analysis is very important for elucidating the functions of proteins because many proteins execute their functions in living…”
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Metal ion-directed cooperative DNA binding of small molecules by Ihara, Toshihiro, Ikegami, Takashi, Fujii, Tomohiro, Kitamura, Yusuke, Sueda, Shinji, Takagi, Makoto, Jyo, Akinori

“…Compound (1), which consists of an oxine and a pyridinium group, was synthesized as a metal-responsive DNA binding ligand. Two 1s coordinate to a Cu(II) to…”
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High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris by Li, Lei, Wang, Jin-Xing, Zhao, Xiao-Fan, Kang, Cui-Jie, Liu, Ning, Xiang, Jian-Hai, Li, Fu-Hua, Sueda, Shinji, Kondo, Hiroki

“…Penaeidins, members of a new family of antimicrobial peptides constitutively produced and stored in the haemocytes of penaeid shrimp, display antimicrobial…”
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Does the nuclear envelope retain its identity during mitosis? by Tsuda, Natsumi, Fukagawa, Ryohei, Sueda, Shinji

“…During mitosis in metazoan species, the nuclear envelope (NE) undergoes breakdown, and its fragments are absorbed within the membranous network of the…”
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A unique biotin carboxyl carrier protein in archaeon Sulfolobus tokodaii by Li, Yan-Qiu, Sueda, Shinji, Kondo, Hiroki, Kawarabayasi, Yutaka

“…Biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl…”
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Structure and Luminescence Properties of the Tetradentate β-Diketonate–Europium(III) Complexes by Yuan, Jingli, Sueda, Shinji, Somazawa, Ryosuke, Matsumoto, Keiichiro, Matsumoto, Kazuko

“…The luminescence properties of Eu3+ complexes with a series of tetradentate β-diketones were compared and it was found that the Eu3+ complexes of…”
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Identification of the Catalytic Residues Involved in the Carboxyl Transfer of Pyruvate Carboxylase by Yong-Biao, Jin, Islam, Md. Nurul, Sueda, Shinji, Kondo, Hiroki

“…To clarify the mechanism of carboxyl transfer from carboxylbiotin to pyruvate, the following conserved amino acid residues present in the carboxyl transferase…”
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Substrate specificity of archaeon Sulfolobus tokodaii biotin protein ligase by Sueda, Shinji, Li, Yan-Qiu, Kondo, Hiroki, Kawarabayasi, Yutaka

“…Biotin protein ligase (BPL) is an enzyme mediating biotinylation of a specific lysine residue of the carboxyl carrier protein (BCCP) of biotin-dependent…”
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Pyridoxine biosynthesis in yeast: participation of ribose 5-phosphate ketol-isomerase by Kondo, Hiroki, Nakamura, Yoriko, Dong, Yi-Xin, Nikawa, Jun-ichi, Sueda, Shinji

“…To identify the genes involved in pyridoxine synthesis in yeast, auxotrophic mutants were prepared. After transformation with a yeast genomic library, a…”
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Immobilization of immunoglobulin-G-binding domain of Protein A on a gold surface modified with biotin ligase by Miyao, Hiroki, Ikeda, Yusuke, Shiraishi, Arata, Kawakami, Yuji, Sueda, Shinji

“…Protein A from Staphylococcus aureus specifically binds to the Fc region of immunoglobulin G (IgG) and is widely used as a scaffold for the immobilization of…”
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Stepwise preparation of a polymer comprising protein building blocks on a solid support for immunosensing platform by Miyao, Hiroki, Uemura, Utaro, Sueda, Shinji

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Ultrasensitive Biotin Assay of a Noncompetitive Format in a Homogeneous Solution Based on Resonance Energy Transfer Induced by a Protein–Protein Interaction by Ikeda, Tomohiro, Miyao, Hiroki, Sueda, Shinji

“…Biotin is a water-soluble vitamin serving as a cofactor for several metabolic enzymes and plays crucial roles in every living cell. In the present study, we…”
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