Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Culture scale-up and immobilisation of a mixed methanotrophic consortium for methane remediation in pilot-scale bio-filters

Robust methanotrophic consortia for methane (CH 4 ) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH 4 removal efficiency and potent...

Full description

Saved in:
Bibliographic Details
Published in:Environmental technology Vol. 38; no. 4; pp. 474 - 482
Main Authors: Karthikeyan, Obulisamy Parthiba, Saravanan, Nadarajan, Cirés, Samuel, Alvarez-Roa, Carlos, Razaghi, Ali, Chidambarampadmavathy, Karthigeyan, Velu, Chinnathambi, Subashchandrabose, Gobalakrishnan, Heimann, Kirsten
Format: Journal Article
Language:English
Published: England Taylor & Francis 16-02-2017
Taylor & Francis Ltd
Subjects:
Air Pollutants, Occupational - metabolism
bio-ball
bio-filter
Byproducts
Coal Mining
Consortia
continuous-stirred tank reactor
Cyanobacteria
Cyanobacteria - metabolism
Enrichment
Filtration
Geologic Sediments - microbiology
marine sediment
Mats
Metal matrix composites
Methane
Methane - metabolism
methanotrophs
Microalgae - metabolism
Microbial Consortia - physiology
Pilot Projects
Product development
Remediation
Tanks
British Isles
Methane
marine sediment
continuous-stirred tank reactor
methanotrophs
bio-ball
bio-filter
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Robust methanotrophic consortia for methane (CH 4 ) remediation and by-product development are presently not readily available for industrial use. In this study, a mixed methanotrophic consortium (MMC), sequentially enriched from a marine sediment, was assessed for CH 4 removal efficiency and potential biomass-generated by-product development. Suitable packing material for bio-filters to support MMC biofilm establishment and growth was also evaluated. The enriched MMC removed ∼7-13% CH 4 under a very high gas flow rate (2.5 L min −1 ; 20-25% CH 4 ) in continuous-stirred tank reactors (∼10 L working volume) and the biomass contained long-chain fatty acids (i.e. C 16 and C 18 ). Cultivation of the MMC on plastic bio-balls abated ∼95-97% CH 4 in pilot-scale non-sterile outdoor-operated bio-filters (0.1 L min −1 ; 1% CH 4 ). Contamination by cyanobacteria had beneficial effects on treating low-level CH 4 , by providing additional oxygen for methane oxidation by MMC, suggesting that the co-cultivation of MMC with cyanobacterial mats does not interfere with and may actually be beneficial for remediation of CH 4 and CO 2 at industrial scale.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0959-3330
1479-487X
DOI:10.1080/09593330.2016.1198424