Femtosecond laser desorption ionization mass spectrometry imaging and multivariate analysis of lipids in pancreatic tissue

Femtosecond laser desorption ionization mass spectrometry was used to obtain mass spectrometric (MS) images of lipids in human pancreatic tissue. The resulting MS images were analyzed using multivariate analysis, specifically principal component analysis and maximum a posteriori (MAP) reconstruction...

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Bibliographic Details
Published in:	Biointerphases Vol. 13; no. 3; p. 03B416
Main Authors:	Walker, Amy V, Gelb, Lev D, Barry, Grant E, Subanajouy, Polatip, Poudel, Ananta, Hara, Manami, Veryovkin, Igor V, Bell, Graeme I, Hanley, Luke
Format:	Journal Article
Language:	English
Published:	United States American Vacuum Society 02-04-2018
Subjects:	Fatty Acids - analysis Humans In Focus: Special Issue on Secondary Ion Mass Spectrometry (SIMS) Mass Spectrometry - methods Multivariate Analysis Pancreas - chemistry Phosphatidylcholines - analysis
Online Access:	Get full text
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Summary:	Femtosecond laser desorption ionization mass spectrometry was used to obtain mass spectrometric (MS) images of lipids in human pancreatic tissue. The resulting MS images were analyzed using multivariate analysis, specifically principal component analysis and maximum a posteriori (MAP) reconstruction. Both analysis methods showed that the MS images can be separated into lipid and non-lipid areas. MAP analysis further indicated that the lipid areas are composed of phosphatidylcholines and fatty acids. However, definitive identification of the lipids cannot be made because none of the intact parent ions of phosphatidylcholine, sphingomyelins, and/or other lipids were observed. The MAP analysis also revealed that the non-lipid areas could be separated into components that are due to the sample chemical treatment and topography.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1934-8630 1559-4106
DOI:	10.1116/1.5016301