Efficacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens

The attenuation of infectious bronchitis (IB) QX-like virus strain L1148 is described. The virus was passaged multiple times in embryonated specific pathogen free (SPF) chicken eggs, and at different passage levels samples were tested for safety for the respiratory tract and kidneys in 1-day-old SPF...

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Bibliographic Details
Published in:	Avian pathology Vol. 40; no. 1; pp. 93 - 102
Main Authors:	Geerligs, H.J, Boelm, G.J, Meinders, C.A.M, Stuurman, B.G.E, Symons, J, Tarres-Call, J, Bru, T, Vila, R, Mombarg, M, Karaca, K, Wijmenga, W, Kumar, M
Format:	Journal Article
Language:	English
Published:	England World Veterinary Poultry Association 01-02-2011 Taylor & Francis Group Taylor & Francis Ltd Taylor & Francis
Subjects:	amino acid substitution Amino acids Animal vaccines Animals antibodies Antibodies, Viral - blood Avian flu chicken eggs Chickens Coronavirus Infections - prevention & control Coronavirus Infections - veterinary Female infectious bronchitis Infectious bronchitis virus Infectious bronchitis virus - immunology Kidneys pathogenicity Poultry Poultry Diseases - prevention & control Respiratory system specific pathogen-free animals Vaccination - veterinary vaccines Vaccines, Attenuated - adverse effects Vaccines, Attenuated - immunology Viral Vaccines - adverse effects Viral Vaccines - immunology viruses
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Summary:	The attenuation of infectious bronchitis (IB) QX-like virus strain L1148 is described. The virus was passaged multiple times in embryonated specific pathogen free (SPF) chicken eggs, and at different passage levels samples were tested for safety for the respiratory tract and kidneys in 1-day-old SPF chickens. There was a clear decrease in pathogenicity for the respiratory tract and kidneys when the virus had undergone a large number of passages. Passage level 80 was investigated for safety for the reproductive tract in 1-day-old and 7-day-old SPF chickens. In 1-day-old chickens, 12.5% of the vaccinated birds had macroscopic lesions. No lesions were observed if the chickens had been vaccinated at 7 days of age. Passage level 80 was investigated for its ability to spread from vaccinated to non-vaccinated chickens and for dissemination in the body. The virus was able to spread from vaccinated chickens to groups of non-vaccinated chickens, and in the vaccinated birds the virus was found frequently in oro-pharyngeal and cloacal swabs. A fragment of the hypervariable region of the S1 protein of passage level 80 was sequenced and revealed nucleotide changes resulting in two amino acid substitutions. Passage level 80 was given additional passages to levels 82 and 85. Both passage levels were tested for efficacy in SPF chickens and passage level 85 was tested for efficacy in commercial chickens with maternally derived antibodies (MDA) against a challenge with QX-like strain IB D388. In both SPF chickens and chickens with MDA, the vaccines based on strain IB L1148 were efficacious against challenge.
Bibliography:	http://dx.doi.org/10.1080/03079457.2010.542742 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0307-9457 1465-3338 1465-3338
DOI:	10.1080/03079457.2010.542742