Using Rapid Prototyping to Develop a Cell-Based Platform with Electrical Impedance Sensor Membranes for In Vitro RPMI2650 Nasal Nanotoxicology Monitoring

Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotox...

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Bibliographic Details
Published in:	Biosensors (Basel) Vol. 14; no. 2; p. 107
Main Authors:	Vasconez Martinez, Mateo Gabriel, Reihs, Eva I, Stuetz, Helene M, Hafner, Astrid, Brandauer, Konstanze, Selinger, Florian, Schuller, Patrick, Bastus, Neus, Puntes, Victor, Frank, Johannes, Tomischko, Wolfgang, Frauenlob, Martin, Ertl, Peter, Resch, Christian, Bauer, Gerald, Povoden, Guenter, Rothbauer, Mario
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-02-2024
Subjects:	Acute toxicity Apoptosis Biochips Biosensors Cell adhesion & migration Cell culture Cytolytic activity Cytotoxicity Electrical impedance Environmental assessment environmental monitoring Environmental risk Impedance impedance sensors lab-on-a-chip Manufacturing Membranes Methods Microfluidics Mucosa Nanomaterials Nanoparticles nanotoxicology nasal epithelial cells Nasal mucosa Physiological aspects Polyethylene terephthalate Rapid prototyping Risk assessment Sensors Silicones Spectrum analysis Testing Toxicity Zinc oxide zinc oxide toxicity Zinc oxides Austria zinc oxide toxicity environmental monitoring nasal epithelial cells impedance sensors lab-on-a-chip nanotoxicology
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Summary:	Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotoxic or cytolytic events using stand-alone biochip platforms in the context of environmental risk assessment. In the current study, we established a nasal mucosa biosensing platform using RPMI2650 mucosa cells inside a membrane-integrated impedance-sensing biochip using exclusively rapid prototyping technologies. In a final proof-of-concept, we applied this biosensing platform to create human cell models of nasal mucosa for monitoring the acute cytotoxic effect of zinc oxide reference nanoparticles. Our data generated with the biochip platform successfully monitored the acute toxicity and cytolytic activity of 6 mM zinc oxide nanoparticles, which was non-invasively monitored as a negative impedance slope on nasal epithelial models, demonstrating the feasibility of rapid prototyping technologies such as additive manufacturing and xurography for cell-based platform development.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2079-6374 2079-6374
DOI:	10.3390/bios14020107