Search Results - "Strychalski, Elizabeth A"

Genetic circuit design automation by Nielsen, Alec A. K., Der, Bryan S., Shin, Jonghyeon, Vaidyanathan, Prashant, Paralanov, Vanya, Strychalski, Elizabeth A., Ross, David, Densmore, Douglas, Voigt, Christopher A.

“…Computation can be performed in living cells by DNA-encoded circuits that process sensory information and control biological functions. Their construction is…”
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Design and synthesis of a minimal bacterial genome by Hutchison, Clyde A., Chuang, Ray-Yuan, Noskov, Vladimir N., Assad-Garcia, Nacyra, Deerinck, Thomas J., Ellisman, Mark H., Gill, John, Kannan, Krishna, Karas, Bogumil J., Ma, Li, Pelletier, James F., Qi, Zhi-Qing, Richter, R. Alexander, Strychalski, Elizabeth A., Sun, Lijie, Suzuki, Yo, Tsvetanova, Billyana, Wise, Kim S., Smith, Hamilton O., Glass, John I., Merryman, Chuck, Gibson, Daniel G., Venter, J. Craig

“…We used whole-genome design and complete chemical synthesis to minimize the 1079-kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial…”
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Organizing genome engineering for the gigabase scale by Bartley, Bryan A., Beal, Jacob, Karr, Jonathan R., Strychalski, Elizabeth A.

“…Genome-scale engineering holds great potential to impact science, industry, medicine, and society, and recent improvements in DNA synthesis have enabled the…”
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Quantitative Measurements of the Size Scaling of Linear and Circular DNA in Nanofluidic Slitlike Confinement by Strychalski, Elizabeth A, Geist, Jon, Gaitan, Michael, Locascio, Laurie E, Stavis, Samuel M

“…Quantitative size measurements of single linear and circular DNA molecules in nanofluidic slitlike confinement are reported. A novel experimental method using…”
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Expanding the Capabilities of Microfluidic Gradient Elution Moving Boundary Electrophoresis for Complex Samples by Strychalski, Elizabeth A, Henry, Alyssa C, Ross, David

“…Gradient elution moving boundary electrophoresis (GEMBE) is a robust, continuous injection separation technique that uses electrophoresis to drive electrically…”
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Comparison of bias and resolvability in single-cell and single-transcript methods by Rammohan, Jayan, Lund, Steven P., Alperovich, Nina, Paralanov, Vanya, Strychalski, Elizabeth A., Ross, David

“…Single-cell and single-transcript measurement methods have elevated our ability to understand and engineer biological systems. However, defining and comparing…”
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Cyberbiosecurity for Biopharmaceutical Products by Mantle, Jennifer L, Rammohan, Jayan, Romantseva, Eugenia F, Welch, Joel T, Kauffman, Leah R, McCarthy, Jim, Schiel, John, Baker, Jeffrey C, Strychalski, Elizabeth A, Rogers, Kelley C, Lee, Kelvin H

“…Cyberbiosecurity is an emerging discipline that addresses the unique vulnerabilities and threats that occur at the intersection of cyberspace and…”
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Genetic requirements for cell division in a genomically minimal cell by Pelletier, James F., Sun, Lijie, Wise, Kim S., Assad-Garcia, Nacyra, Karas, Bogumil J., Deerinck, Thomas J., Ellisman, Mark H., Mershin, Andreas, Gershenfeld, Neil, Chuang, Ray-Yuan, Glass, John I., Strychalski, Elizabeth A.

“…Genomically minimal cells, such as JCVI-syn3.0, offer a platform to clarify genes underlying core physiological processes. Although this minimal cell includes…”
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Cellular mechanics during division of a genomically minimal cell by Pelletier, James F, Glass, John I, Strychalski, Elizabeth A

“…Genomically minimal cells, such as JCVI-syn3.0 and JCVI-syn3A, offer an empowering framework to study relationships between genotype and phenotype. With a…”
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Equilibrium free energies from non-equilibrium trajectories with relaxation fluctuation spectroscopy by Ross, David, Strychalski, Elizabeth A., Jarzynski, Christopher, Stavis, Samuel M.

“…Recent advances in non-equilibrium statistical mechanics and single-molecule measurements have enabled the determination of equilibrium free energies from…”
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A localized transition in the size variation of circular DNA in nanofluidic slitlike confinement by Strychalski, Elizabeth A., Stavis, Samuel M., Geist, Jon

“…We report strong evidence for a localized transition in the size variation of circular DNA between strong and moderate regimes of slitlike confinement. A novel…”
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Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production by Romantseva, Eugenia F, Tack, Drew S, Alperovich, Nina, Ross, David, Strychalski, Elizabeth A

“…Performance variability is a common challenge in cell-free protein production and hinders a wider adoption of these systems for both research and…”
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Cotranscriptionally encoded RNA strand displacement circuits by Schaffter, Samuel W, Strychalski, Elizabeth A

“…Engineered molecular circuits that process information in biological systems could address emerging human health and biomanufacturing needs. However, such…”
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Rise of synthetic yeast: Charting courses to new applications by Taghon, Geoffrey J, Strychalski, Elizabeth A

“…Microbes have long provided us with important capabilities, and the genome engineering of microbes has greatly empowered research and applications in…”
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Design Approaches to Expand the Toolkit for Building Cotranscriptionally Encoded RNA Strand Displacement Circuits by Schaffter, Samuel W., Wintenberg, Molly E., Murphy, Terence M., Strychalski, Elizabeth A.

“…Cotranscriptionally encoded RNA strand displacement (ctRSD) circuits are an emerging tool for programmable molecular computation, with potential applications…”
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Advancing reproducibility can ease the ‘hard truths’ of synthetic biology by Lux, Matthew W, Strychalski, Elizabeth A, Vora, Gary J

“…Abstract Reproducibility has been identified as an outstanding challenge in science, and the field of synthetic biology is no exception. Meeting this challenge…”
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Special issue: reproducibility in synthetic biology by Lux, Matthew W, Strychalski, Elizabeth A, Vora, Gary J

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Building Synthetic CellsFrom the Technology Infrastructure to Cellular Entities by Rothschild, Lynn J., Averesch, Nils J. H., Strychalski, Elizabeth A., Moser, Felix, Glass, John I., Cruz Perez, Rolando, Yekinni, Ibrahim O., Rothschild-Mancinelli, Brooke, Roberts Kingman, Garrett A., Wu, Feilun, Waeterschoot, Jorik, Ioannou, Ion A., Jewett, Michael C., Liu, Allen P., Noireaux, Vincent, Sorenson, Carlise, Adamala, Katarzyna P.

“…The de novo construction of a living organism is a compelling vision. Despite the astonishing technologies developed to modify living cells, building a…”
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Effects of DNA template preparation on variability in cell-free protein production by Romantseva, Eugenia, Alperovich, Nina, Ross, David, Lund, Steven P, Strychalski, Elizabeth A

“…Abstract DNA templates for protein production remain an unexplored source of variability in the performance of cell-free expression (CFE) systems. To…”
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Diffusion of DNA in Nanoslits by Strychalski, Elizabeth A, Levy, Stephen L, Craighead, Harold G

“…We observed the free diffusion of individual 10 kbp, (1/2)λ (24.2 kbp), and λ (48.5 kbp) double-stranded deoxyribonucleic acid (DNA) molecules in fused-silica…”
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