Chronic sensory contact with subordinated conspecifics promotes splenic glucocorticoid resistance in experimentally wounded C57BL/6N male mice

Chronic sensory contact with subordinated conspecifics promotes splenic glucocorticoid resistance in experimentally wounded C57BL/6N male mice

Chronic psychosocial stress induced by the chronic subordinate colony housing (CSC, 19 Days) paradigm promotes functional splenic in vitro glucocorticoid (GC) resistance, but only if associated with significant bite wounding or prior abdominal transmitter implantation. Moreover, sensory contact to s...

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Bibliographic Details
Published in:Scientific reports Vol. 14; no. 1; p. 10867
Main Authors: Schiele, Jessica, Mazzari, Giulia, Struck, Antonia, Bailer, Yorick, Langgartner, Dominik, Reber, Stefan O.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 13-05-2024
Nature Publishing Group
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Subjects:
631/250/256
631/378/1831
Animal models
Animals
Cell viability
Chronic psychosocial stress
Chronic subordinate colony housing (CSC)
Conspecifics
Disease Models, Animal
Glucocorticoid resistance
Glucocorticoids
Housing
Humanities and Social Sciences
Hypotheses
Male
Mice
Mice, Inbred C57BL
multidisciplinary
Phenotypes
Post traumatic stress disorder
Science
Science (multidisciplinary)
Sensory contact
Social Defeat
Social interactions
Spleen
Spleen - metabolism
Statistical analysis
Stress, Psychological
Surgery
Thymus gland
Wounding
Wounding
Chronic subordinate colony housing (CSC)
Glucocorticoid resistance
Chronic psychosocial stress
Sensory contact
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Summary:Chronic psychosocial stress induced by the chronic subordinate colony housing (CSC, 19 Days) paradigm promotes functional splenic in vitro glucocorticoid (GC) resistance, but only if associated with significant bite wounding or prior abdominal transmitter implantation. Moreover, sensory contact to social defeat of conspecifics represents a social stressor for the observer individual. As the occurence and severity of bite wounding is not adequately controllable, the present study aimed to develop an animal model, allowing a bite wound-independent, more reliable generation of chronically-stressed mice characterized by functional splenic in vitro GC resistance. Therefore, male C57BL/6N mice received a standardized sterile intraperitoneal (i.p.) incision surgery or SHAM treatment one week prior to 19-days of (i) CSC, (ii) witnessing social defeat during CSC exposure in sensory contact (SENS) or (iii) single-housing for control (SHC), before assessing basal and LPS-induced splenic in vitro cell viability and GC resistance . Our results indicate that individually-housed SENS but not CSC mice develop mild signs of splenic in vitro GC resistance, when undergoing prior i.p.-wounding. Taken together and considering that future studies are warranted, our findings support the hypothesis that the combination of repeated standardized i.p.-wounding with chronic sensory stress exposure represents an adequate tool to induce functional splenic in vitro GC resistance independent of the occurrence of uncontrollable bite wounds required in social stress paradigms to induce a comparable phenotype.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-61581-7