Search Results - "Stroupe, Christopher"

This Is the End: Regulation of Rab7 Nucleotide Binding in Endolysosomal Trafficking and Autophagy by Stroupe, Christopher

“…Rab7 - or in yeast, Ypt7p - governs membrane trafficking in the late endocytic and autophagic pathways. Rab7 also regulates mitochondrion-lysosome contacts,…”
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The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane by Ho, Ruoya, Stroupe, Christopher

“…Many Rab GTPase effectors are membrane-tethering factors, that is, they physically link two apposed membranes before intracellular membrane fusion. In this…”
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The HOPS/Class C Vps Complex Tethers High‐Curvature Membranes via a Direct Protein–Membrane Interaction by Ho, Ruoya, Stroupe, Christopher

“…Membrane tethering is a physical association of two membranes before their fusion. Many membrane tethering factors have been identified, but the interactions…”
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In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties by Kreutzberger, Alex J. B., Kiessling, Volker, Stroupe, Christopher, Liang, Binyong, Preobraschenski, Julia, Ganzella, Marcelo, Kreutzberger, Mark A. B., Nakamoto, Robert, Jahn, Reinhard, Castle, J. David, Tamm, Lukas K.

“…Regulated exocytosis of synaptic vesicles is substantially faster than of endocrine dense core vesicles despite similar molecular machineries. The reasons for…”
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Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion by Zick, Michael, Stroupe, Christopher, Orr, Amy, Douville, Deborah, Wickner, William T

“…Like other intracellular fusion events, the homotypic fusion of yeast vacuoles requires a Rab GTPase, a large Rab effector complex, SNARE proteins which can…”
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Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p by Stroupe, C, Collins, K.M, Fratti, R.A, Wickner, W

“…Coupling of Rab GTPase activation and SNARE complex assembly during membrane fusion is poorly understood. The homotypic fusion and vacuole protein sorting…”
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The Major Role of the Rab Ypt7p in Vacuole Fusion Is Supporting HOPS Membrane Association by Hickey, Christopher M., Stroupe, Christopher, Wickner, William

“…Yeast vacuole fusion requires soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), the Rab GTPase Ypt7p, vacuolar lipids, Sec17p…”
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Minimal membrane docking requirements revealed by reconstitution of Rab GTPase-dependent membrane fusion from purified components by Stroupe, Christopher, Hickey, Christopher M, Mima, Joji, Burfeind, Amy S, Wickner, William

“…Rab GTPases and their effectors mediate docking, the initial contact of intracellular membranes preceding bilayer fusion. However, it has been unclear whether…”
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Correction: Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion by Zick, Michael, Stroupe, Christopher, Orr, Amy, Douville, Deborah, Wickner, William T

“…Membranes linked by trans-SNARE complexes require lipids prone to non-bilayer structure for progression to fusion. eLife 3:e01879. doi: 10.7554/eLife.01879…”
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Crystal Structures of a Rab Protein in its Inactive and Active Conformations by Stroupe, Christopher, Brunger, Axel T.

“…We have determined crystal structures of Sec4, a member of the Rab family in the G protein superfamily, in two states: bound to GDP, and to a non-hydrolyzable…”
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The HOPS/Class C Vps Complex Tethers Membranes via a Direct Protein-Membrane Interaction by Stroupe, Christopher

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The HOPS/Class C Vps Complex Tethers Membranes by Binding to a Rab Gtpase in one Membrane and Directly to a Second Membrane via a Curvature-Sensing Motif by Stroupe, Christopher

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The yeast vacuolar Rab GTPase Ypt7p has an activity beyond membrane recruitment of the homotypic fusion and protein sorting-Class C Vps complex by Stroupe, Christopher

“…A previous report described lipid mixing of reconstituted proteoliposomes made using lipid mixtures that mimic the composition of yeast vacuoles. This lipid…”
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Autophagy: Cells SNARE Selves by Stroupe, Christopher

“…Autophagosomes sequester cytosolic constituents and deliver this cargo to lysosomes for destruction. Two groups now report that autophagosome maturation…”
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Non-Boltzmann thermodynamic integration (NBTI) for macromolecular systems: Relative free energy of binding of trypsin to benzamidine and benzylamine by Ota, Nobuyuki, Stroupe, Christopher, Ferreira-da-Silva, J.M.S., Shah, Sapan A., Mares-Guia, Marcos, Brunger, Axel T.

“…The relative free energies of binding of trypsin to two amine inhibitors, benzamidine (BZD) and benzylamine (BZA), were calculated using non‐Boltzmann…”
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Crystal structures of Sec4 bound to GDP and to a nonhydrolyzable GTP analog by Stroupe, Stephen Christopher

“…Rab proteins constitute a family of small G proteins essential for membrane trafficking and vesicular transport in eukaryotic cells. I have determined the…”
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