O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation
Gut microbiota of the gastrointestinal tract provide health benefits to the human host via bacterial metabolites. Bacterial butyrate has beneficial effects on intestinal homeostasis and is the preferred energy source of intestinal epithelial cells, capable of inducing differentiation. It was previou...
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| Published in: | Molecular & cellular proteomics Vol. 22; no. 3; p. 100501 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Elsevier Inc
01-03-2023
American Society for Biochemistry and Molecular Biology |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Gut microbiota of the gastrointestinal tract provide health benefits to the human host via bacterial metabolites. Bacterial butyrate has beneficial effects on intestinal homeostasis and is the preferred energy source of intestinal epithelial cells, capable of inducing differentiation. It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2 (Cancer Coli-2), by applying porous graphitized carbon nano–liquid chromatography with electrospray ionization tandem mass spectrometry. Moreover, a quantitative proteomic approach was used to decipher changes in the cell proteome. It was found that the fully differentiated butyrate-stimulated cells are characterized by a higher expression of sialylated O-glycan structures, whereas fucosylation is downregulated with differentiation. By performing an integrative approach, we generated hypotheses about the origin of the observed O-glycome changes. These insights pave the way for future endeavors to study the dynamic O-glycosylation patterns in the gut, either produced via cellular biosynthesis or through the action of bacterial glycosidases as well as the functional role of these patterns in homeostasis and dysbiosis at the gut–microbiota interface.
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•Butyrate induces changes in the CaCo-2 O-glycome and proteome upon differentiation.•Differentiated cells show a higher expression of sialylated O-glycan structures.•Terminal fucosylation shows lower expression in the differentiated cells.•Butyrate induces changes in monosaccharide conversion and cell adhesion proteome.
Bacterial butyrate is beneficial for intestinal homeostasis as the preferred energy source of intestinal epithelia, capable of inducing differentiation. Here, we describe glycomic and proteomic signatures of butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2. We identified an upregulation of specific O-glycan signatures as well as specific proteins involved in cell metabolism, monosaccharide precursor biosynthesis, and cell adhesion. Using an integrative approach, we generated hypotheses about the origin of the glycosylation changes with differentiation. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1535-9476 1535-9484 1535-9484 |
| DOI: | 10.1016/j.mcpro.2023.100501 |