The role of IL-5, IL-6 and IL-10 in primary and vaccine-primed immune responses to infection with Friend retrovirus (Murine leukaemia virus)

Institut für Virologie der Universität Würzburg, Versbacher Str.7, 97078 Würzburg, Germany 1 Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, MT 59840, Hamilton, USA 2 Author for correspondence...

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Published in:	Journal of general virology Vol. 82; no. 6; pp. 1349 - 1354
Main Authors:	Strestik, Beatrice D, Olbrich, Anke R. M, Hasenkrug, Kim J, Dittmer, Ulf
Format:	Journal Article
Language:	English
Published:	England Soc General Microbiol 01-06-2001
Subjects:	Animals Antibodies, Viral - immunology Friend murine leukaemia virus Friend murine leukemia virus Friend murine leukemia virus - immunology Friend murine leukemia virus - physiology Gene Deletion Immunoglobulin G - immunology Interleukin-10 - deficiency Interleukin-10 - genetics Interleukin-10 - immunology Interleukin-5 - deficiency Interleukin-5 - genetics Interleukin-5 - immunology Interleukin-6 - deficiency Interleukin-6 - genetics Interleukin-6 - immunology Leukemia, Experimental - immunology Leukemia, Experimental - prevention & control Leukemia, Experimental - virology Mice Mice, Inbred C57BL Neutralization Tests Retroviridae Infections - immunology Retroviridae Infections - virology Retrovirus Spleen - immunology Spleen - virology Tumor Virus Infections - immunology Tumor Virus Infections - virology Vaccination Vaccines, Attenuated - immunology Viral Load Viral Vaccines - immunology Virus Replication
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Summary:	Institut für Virologie der Universität Würzburg, Versbacher Str.7, 97078 Würzburg, Germany 1 Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, MT 59840, Hamilton, USA 2 Author for correspondence: Ulf Dittmer. Fax +49 931 2013934. e-mail ulf.dittmer{at}mail.uni-wuerzburg.de The defence of a host against viral infections is strongly influenced by cytokines. We investigated the role of the B-cell stimulating cytokines IL-5 and IL-6, and the immuno-suppressive cytokine IL-10, during primary and secondary immune responses in mice against infection with Friend retrovirus (FV) ( Murine leukaemia virus ). IL-5 -/- mice were comparable to C57BL/6 wild-type mice in their ability to control acute FV infection. In contrast, IL-6 -/- and IL-10 -/- mice showed significantly enhanced virus loads in spleen cells. However, this impaired control of acute FV replication did not alter the long-term control over persistent FV in IL-6 -/- and IL-10 -/- mice. Immunization with a live attenuated vaccine virus prior to challenge protected all three types of cytokine-deficient mice from high levels of spleen virus, despite the finding that the vaccinated IL-5- and IL-6-deficient mice had significantly reduced titres of virus-neutralizing IgG class antibodies. The results indicate that IL-6 and IL-10 contribute to primary immune responses against FV, but are dispensable during persistent infection and vaccine-primed secondary responses.
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ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-82-6-1349