Search Results - "Stoward, J"

Quantitative evaluation of the beneficial effects in the mdx mouse of epigallocatechin gallate, an antioxidant polyphenol from green tea by Nakae, Yoshiko, Dorchies, Olivier M., Stoward, Peter J., Zimmermann, Benno F., Ritter, Christina, Ruegg, Urs T.

“…In two separate previous studies, we reported that subcutaneous (sc) or oral administration of (−)-epigallocatechin-3-gallate (EGCG) limited the development of…”
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Subcutaneous injection, from birth, of epigallocatechin-3-gallate, a component of green tea, limits the onset of muscular dystrophy in mdx mice: a quantitative histological, immunohistochemical and electrophysiological study by Nakae, Yoshiko, Hirasaka, Katsuya, Goto, Junpei, Nikawa, Takeshi, Shono, Masayuki, Yoshida, Mizuko, Stoward, Peter J.

“…Dystrophic muscles suffer from enhanced oxidative stress. We have investigated whether administration of an antioxidant, epigallocatechin-3-gallate (EGCG), a…”
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The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies by Nakae, Yoshiko, Stoward, Peter J.

“…Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice…”
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A new technique for the quantitative assessment of 8-oxoguanine in nuclear DNA as a marker of oxidative stress. Application to dystrophin-deficient DMD skeletal muscles by Nakae, Yoshiko, Stoward, Peter J, Bespalov, Ivan A, Melamede, Robert J, Wallace, Susan S

“…This is the first report on the development of an immunohistochemical technique, combined with quantitative image analysis, for the assessment of oxidative…”
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Early onset of lipofuscin accumulation in dystrophin-deficient skeletal muscles of DMD patients and mdx mice by Nakae, Yoshiko, Stoward, Peter J, Kashiyama, Tatsuo, Shono, Masayuki, Akagi, Akiko, Matsuzaki, Tetsuya, Nonaka, Ikuya

“…Lipofuscin, the so-called ageing pigment, is formed by the oxidative degradation of cellular macromolecules by oxygen-derived free radicals and redox-active…”
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A valid quantitative histochemical technique for assaying cytochrome c oxidase in single cells by Nakae, Y, Stoward, P J

“…A quantitative histochemical method for assaying cytochrome c oxidase (COX) has been validated with two new findings concerning the optimal tissue thickness…”
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Localisation and quantification of dehydrogenase activities in single muscle fibers of mdx gastrocnemius by Nakae, Y, Stoward, P J, Shono, M, Matsuzaki, T

“…The kinetics of succinate (SDH) and lactate (LDH) dehydrogenases were determined in single muscle fibres in unfixed sections of the gastrocnemius of dystrophic…”
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Kinetic Parameters of Lactate Dehydrogenase in Liver and Gastrocnemius Determined by Three Quantitative Histochemical Methods by Nakae, Yoshiko, Stoward, Peter J

“…We determined the Michaelis constant (K m) and maximal velocity (V max) of lactate dehydrogenase (LDH) in periportal hepatocytes and skeletal muscle fibers by…”
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Decrease in human quadriceps muscle protein turnover consequent upon leg immobilization by Gibson, J N, Halliday, D, Morrison, W L, Stoward, P J, Hornsby, G A, Watt, P W, Murdoch, G, Rennie, M J

“…Quadriceps muscle protein turnover was assessed in the post-absorptive state in six men immediately after the end of unilateral leg immobilization (37 +/- 4…”
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Most apoptotic cells in mdx diaphragm muscle contain accumulated lipofuscin by Nakae, Y, Stoward, P J, Shono, M, Matsuzaki, T

“…An age-related pigment, lipofuscin (LF), which accumulates in postmitotic, long-lived cells, is formed by the oxidative degradation of cellular macromolecules…”
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Effects of Tissue Protectants on the Kinetics of Lactate Dehydrogenase in Cells by Nakae, Yoshiko, Stoward, Peter J

“…We studied the effects of two tissue protectants, polyvinyl alcohol (PVA) and agarose gel, on a kinetic parameter of lactate dehydrogenase LDH that is assumed…”
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Most apoptotic cells in mdx diaphragm muscle contain accumulated lipofuscin by Nakae, Yoshiko, Stoward, Peter J., Shono, Masayuki, Matsuzaki, Tetsuya

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The kinetics of enzymes in situ, with special reference to lactate and succinate dehydrogenases by Nakae, Y, Stoward, P J

“…The kinetics of two enzyme systems in situ that have been studied with real-time image analysis systems are reviewed in detail. The enzymes are a…”
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Effects of therapeutic percutaneous electrical stimulation of atrophic human quadriceps on muscle composition, protein synthesis and contractile properties by Gibson, J N, Morrison, W L, Scrimgeour, C M, Smith, K, Stoward, P J, Rennie, M J

“…The effects of percutaneous electrical stimulation (70 V, 300 microseconds pulses at 30 Hz) on muscle composition and rate of protein synthesis were studied in…”
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The diverse Michaelis constants and maximum velocities of lactate dehydrogenase in situ in various types of cell by Nakae, Y, Stoward, P J

“…The kinetics of lactate dehydrogenase in mouse cardiac muscle fibres, skeletal muscle fibres, gastric parietal cells, parotid gland ductal and acinar cells,…”
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The everchanging advances in enzyme histochemistry, 1986-1996 by Stoward, P J, Nakae, Y, Van Noorden, C J

“…The principal developments in the field of enzyme histochemistry in the ten years since 1986 are reviewed briefly. They include the replacement of catalytic…”
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The immunohistolocalization of carbonic anhydrase in rodent tissues by Spicer, SS, Stoward, PJ, Tashian, RE

“…Carbonic anhydrase has been localized with an immunoenzyme bridge technique in the following sites in paraffin sections of fixed rodent tissues: gastric…”
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The initial reaction velocities of lactate dehydrogenase in various cell types by Nakae, Y, Stoward, P J

“…The initial reaction velocities (vi) of lactate dehydrogenase in hepatocytes, cardiac muscle fibres, skeletal (gastrocnemius) muscle fibres, gastric parietal…”
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Initial reaction kinetics of succinate dehydrogenase in mouse liver studied with a real-time image analyser system by Nakae, Y, Stoward, P J

“…The initial reaction kinetics of succinate dehydrogenase in situ were investigated in sections of mouse unfixed liver using an ARGUS-100 image analyser system…”
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Estimating the initial reaction velocity of a soluble dehydrogenase in situ by Nakae, Y, Stoward, P J

“…The initial reaction velocities (vi) of lactate dehydrogenase in single hepatocytes were determined, by microdensitometry or computer-assisted image analysis,…”
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