Identification of two mosquitocidal Bacillus cereus strains showing different host ranges

Mosquitocidal bacteria, M413 and C32 have been isolated from sediment samples collected from woodland and ditch, respectively. Gas chromatographic analysis of fatty acids methyl esters (GC-FAME) and 16S rRNA gene sequence alignment results showed these isolates belong to Bacillus cereus. The SDS–PAG...

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Bibliographic Details
Published in:	Journal of invertebrate pathology Vol. 100; no. 1; pp. 54 - 56
Main Authors:	Park, Hyun-Woo, Hayes, Sabrina R., Stout, Gail M., Day-Hall, Ginny, Latham, Mark D., Hunter, John P.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 2009 Elsevier
Subjects:	animal parasites and pests Animals Bacillus cereus Bacillus cereus - chemistry Bacillus cereus - isolation & purification Bacillus cereus - physiology Base Sequence Biological and medical sciences biological control biological control agents Chromatography, Gas Culex quinquefasciatus Culicidae Culicidae - drug effects Culicidae - growth & development Electrophoresis, Polyacrylamide Gel Endotoxins - chemistry Endotoxins - toxicity entomopathogenic bacteria Fundamental and applied biological sciences. Psychology Geologic Sediments - microbiology host range hosts insect pests instars Larva - drug effects Larva - growth & development larvae Lethal Dose 50 Medically important nuisances and vectors, pests of stored products and materials: population survey and control molecular sequence data Mosquitocidal nucleotide sequences Ochlerotatus Ochlerotatus taeniorhynchus Pest Control, Biological ribosomal RNA RNA, Bacterial - chemistry RNA, Ribosomal - chemistry Sequence Alignment Sequence Analysis, RNA species differences sporulation strain differences strains Vectors. Intermediate hosts Mosquitocidal Ochlerotatus taeniorhynchus Bacillus cereus Culex quinquefasciatus Entomopathogen Disease Insecta Parasite Bacillaceae Bacillales Arthropoda Culicidae Host range Bacteria Ectoparasite Invertebrata Vector Diptera
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Summary:	Mosquitocidal bacteria, M413 and C32 have been isolated from sediment samples collected from woodland and ditch, respectively. Gas chromatographic analysis of fatty acids methyl esters (GC-FAME) and 16S rRNA gene sequence alignment results showed these isolates belong to Bacillus cereus. The SDS–PAGE analysis of sporulated cultures of both isolates showed two major bands very similar in size. Interestingly, however, M413 is mainly toxic to 4th instars of Ochlerotatus taeniorhynchus whereas C32 is to those of Culex quinquefasciatus.
Bibliography:	http://dx.doi.org/10.1016/j.jip.2008.09.010 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2008.09.010