Anti-Müllerian hormone is a survival factor and promotes the growth of rhesus macaque preantral follicles during matrix-free culture

Anti-Müllerian hormone is a survival factor and promotes the growth of rhesus macaque preantral follicles during matrix-free culture

Anti-Müllerian hormone (AMH) plays a key role during ovarian follicular development, with local actions associated with a dynamic secretion profile by growing follicles. While results for AMH effects on antral follicle growth and function are consistent among studies in various species, any effects...

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Bibliographic Details
Published in:Biology of reproduction Vol. 98; no. 2; pp. 197 - 207
Main Authors: Xu, Jing, Xu, Fuhua, Lawson, Maralee S, Tkachenko, Olena Y, Ting, Alison Y, Kahl, Christoph A, Park, Byung S, Stouffer, Richard R, Bishop, Cecily V
Format: Journal Article
Language:English
Published: United States Society for the Study of Reproduction 01-02-2018
Oxford University Press
Subjects:
Animals
Anti-Mullerian Hormone - genetics
Anti-Mullerian Hormone - metabolism
anti-Müllerian hormone
Female
Fertility
follicle
follicle culture
Gene expression
In Vitro Oocyte Maturation Techniques - methods
Macaca mulatta
Monkeys & apes
Ovarian Follicle - growth & development
Ovarian Follicle - metabolism
OVARY
primate
Reproductive health
primate
ovary
follicle culture
anti-Müllerian hormone
follicle
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Summary:Anti-Müllerian hormone (AMH) plays a key role during ovarian follicular development, with local actions associated with a dynamic secretion profile by growing follicles. While results for AMH effects on antral follicle growth and function are consistent among studies in various species, any effects on preantral follicle development remain controversial. Therefore, experiments were conducted to investigate the direct actions and role of AMH during follicle development at the preantral stage. Macaque-specific short-hairpin RNAs (shRNAs) targeting AMH mRNA were incorporated into adenoviral vectors to decrease AMH gene expression in rhesus macaque follicles. Secondary follicles were isolated from adult macaque ovaries and cultured individually in the ultra-low-attachment dish containing defined medium supplemented with follicle-stimulating hormone and insulin for 5 weeks. Follicles were randomly assigned to treatment groups: (a) control, (b) nontargeting control shRNA-vector, (c) AMH shRNA-vector, (d) AMH shRNA-vector + recombinant human AMH, and (e) recombinant human AMH. Follicle survival and growth were assessed. Culture media were analyzed for steroid hormone and paracrine factor concentrations. For in vivo study, the nontargeting control shRNA-vector and AMH shRNA-vector were injected into macaque ovaries. Ovaries were collected 9 days postinjection for morphology and immunohistochemistry assessment. Decreased AMH expression reduced preantral follicle survival and growth in nonhuman primates. Supplemental AMH treatment in the culture media promoted preantral follicle growth to the small antral stage in vitro with increased steroid hormone and paracrine factor production, as well as oocyte maturation. These data demonstrate that AMH is a critical follicular paracrine/autocrine factor positively impacting preantral follicle survival and growth in primates. Summary Sentence Anti-Müllerian hormone is a survival factor for preantral follicles in nonhuman primates, and promotes preantral follicle growth to the small antral stage with increased steroid hormone and paracrine factor production, as well as oocyte maturation.
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ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/iox181