Survival of Vibrio vulnificus in shellstock and shucked oysters (Crassostrea gigas and Crassostrea virginica) and effects of isolation medium on recovery

When two species of shellstock oysters were artifically contaminated with Vibrio vulnificus, the bacterium survived when the oysters were stored at 10 degrees C and below. Large numbers of endogenous V. vulnificus cells were found after 7 days at both 0.5 and 10 degrees C in uninoculated control oys...

Full description

Saved in:

Bibliographic Details
Published in:	Applied and Environmental Microbiology Vol. 55; no. 12; pp. 3072 - 3079
Main Authors:	Kaysner, C.A. (U.S. Food and Drug Administration, Bothell, WA), Tamplin, M.L, Wekell, M.M, Stott, R.F, Colburn, K.G
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-12-1989
Subjects:	ALMACENAMIENTO Animals Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony Count, Microbial Culture Media Food Microbiology Fundamental and applied biological sciences. Psychology HUITRE Mice Microbiology OSTRA Ostreidae - microbiology STOCKAGE SUPERVIVENCIA SURVIE TEMPERATURA TEMPERATURE VIBRIO Vibrio - growth & development Vibrio - isolation & purification Vibrio - pathogenicity Culture medium Solid medium Temperature Vibrio vulnificus Survival Contamination Enumeration(counting) Storage Bivalvia Crassostrea virginica Medium effect Bacteria Vibrionaceae Isolation Invertebrata Mollusca Crassostrea gigas
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	When two species of shellstock oysters were artifically contaminated with Vibrio vulnificus, the bacterium survived when the oysters were stored at 10 degrees C and below. Large numbers of endogenous V. vulnificus cells were found after 7 days at both 0.5 and 10 degrees C in uninoculated control oysters (Crassostrea virginica). Oysters allowed to take up V. vulnificus from seawater retained the bacterium for 14 days at 2 degrees C. The presence of V. vulnificus in the drip exuded from the shellstock presented a possibility of contamination of other shellstock in storage. V. vulnificus injected into shucked Pacific (Crassostrea gigas) and Eastern (C. virginica) oysters survived at 4 degrees C for at least 6 days. An 18-h most-probable-number enrichment step in alkaline peptone water gave higher recovery levels of V. vulnificus than did direct plating to selective agars. The survival of this pathogen in both shellstock and shucked oysters suggests a potential for human illness, even though the product is refrigerated
Bibliography:	Q03 9026772
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.55.12.3072-3079.1989