Use of multiple endpoints to assess the impact of captivity on gut flora diversity in Long Island Sound Fundulus heteroclitus

Use of multiple endpoints to assess the impact of captivity on gut flora diversity in Long Island Sound Fundulus heteroclitus

We evaluated the impact of short-term captive housing on gut flora diversity in Fundulus heteroclitus by characterizing culturable gut bacteria immediately after fish capture and following short-term (28-day) captive housing. Bacterial colony and cell morphology served as crude but statistically sig...

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Bibliographic Details
Published in:Environmental biology of fishes Vol. 105; no. 7; pp. 867 - 883
Main Authors: Battaglia, Joseph P., Kearney, Christian M., Guerette, Kirsten, Corbishley, Jordan, Sanchez, Emily, Kent, Brennah, Storie, Hanna, Sharp, Emma, Martin, Shane, Saberito, Matthew, Blake, Jonathan D., Feinn, Richard S., Mital, Jeffrey, Kaplan, Lisa A. E.
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-07-2022
Springer Nature B.V
Subjects:
Animal Systematics/Taxonomy/Biogeography
Aquariums
Bacteria
Biomedical and Life Sciences
Captivity
Cell morphology
Colonies
Cytology
Dominance
Environment
Fish
Flora
Food
Freshwater & Marine Ecology
Fundulus heteroclitus
Housing
Intestinal flora
Intestinal microflora
Laboratories
Life Sciences
Nature Conservation
Plants
Pollutants
rRNA 16S
Species richness
Statistical analysis
Zoology
Captivity
Gut flora
Diversity
Microbiome
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Summary:We evaluated the impact of short-term captive housing on gut flora diversity in Fundulus heteroclitus by characterizing culturable gut bacteria immediately after fish capture and following short-term (28-day) captive housing. Bacterial colony and cell morphology served as crude but statistically significant endpoints for detecting alteration in culturable gut bacteria following F. heteroclitus short-term captive housing. 16S rRNA sequence analyses of isolates from short-term captive-housed F. heteroclitus produced fewer families (68%), genera (69%), and species (63%) identities resulting in lower species richness (63%), 2.4-fold lower Margalef’s Index, 1.7-fold lower Menhinick’s Index, 28.6% lower Shannon–Wiener Diversity Index, 2.1-fold greater Simpson Dominance Index, 1.5-fold greater Berger-Parker Dominance, and 2.9-fold lower Fisher’s Alpha Diversity Index. When comparing isolate populations from field and short-term captive-housed fish, Sorenson’s Similarity Coefficient (CC) was 0.081, and Jaccard’s Distance Coefficient (1-J) was 0.958. Based upon these observations, our study provides clear evidence for the substantial and negative impact of short-term captive housing on F. heteroclitus gut flora diversity.
ISSN:0378-1909
1573-5133
DOI:10.1007/s10641-022-01293-x