Search Results - "Storey, B. T."

Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa by Alvarez, J G, Storey, B T

“…Intact human sperm incorporated radiolabelled fatty acids into membrane phospholipids when incubated in medium containing bovine serum albumin as a fatty acid…”
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Comparison of Glycerol, Other Polyols, Trehalose, and Raffinose to Provide a Defined Cryoprotectant Medium for Mouse Sperm Cryopreservation by Storey, Bayard T., Noiles, Esther E., Thompson, Kathleen A.

“…Most procedures for mouse sperm cryopreservation have utilized raffinose to provide hypertonicity for cell desiccation prior to freezing and glycerol to block…”
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Activation of a Gi protein in mouse sperm membranes by solubilized proteins of the zona pellucida, the egg's extracellular matrix by WARD, C. R, STOREY, B. T, KOPF, G. S

“…Zona pellucida (ZP)-induced acrosomal exocytosis in mammalian spermatozoa is thought to be mediated by signal transduction cascades similar to those found in…”
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Human sperm glutathione reductase activity in situ reveals limitation in the glutathione antioxidant defense system due to supply of NADPH by Storey, Bayard T., Alvarez, Juan G., Thompson, Kathleen A.

“…In order to characterize further the antilipoperoxidative enzyme system of human sperm, that part of the system designed to provide reducing equivalents for…”
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Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility by Alvarez, J.G, Storey, B.T

“…Loss of forward motility of rabbit epididymal spermatozoa in high K+ phosphate buffer is inhibited by taurine, hypotaurine, epinephrine and bovine serum…”
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Centrifugation of human spermatozoa induces sublethal damage; separation of human spermatozoa from seminal plasma by a dextran swim-up procedure without centrifugation extends their motile lifetime by Alvarez, J G, Lasso, J L, Blasco, L, Nuñez, R C, Heyner, S, Caballero, P P, Storey, B T

“…While washing of human sperm cells by centrifugation and resuspension is a procedure in widespread use, there have been indications that this procedure per se…”
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Selective activation of Gi1 and Gi2 in mouse sperm by the zona pellucida, the egg's extracellular matrix by WARD, C. R, STOREY, B. T, KOPF, G. S

“…Mammalian sperm acrosomal exocytosis as induced by the egg's zona pellucida (ZP) appears to be a G protein-mediated event. Previously, we demonstrated that…”
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Bicarbonate is essential for fertilization of mouse eggs: mouse sperm require it to undergo the acrosome reaction by LEE, M. A, STOREY, B. T

“…Fertilization of mouse eggs by capacitated mouse sperm occurs readily in vitro in a defined culture medium designated medium CM, derived from Krebs-Ringer…”
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Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa by Storey, B T

“…Lipid peroxidation occurs in human sperm cells with damage to the cell plasma membrane, leading to loss of cytosolic components and hence to cell 'death'. The…”
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Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation by Alvarez, J. G, Storey, B. T

“…Cryopreservation of human sperm, now generally required in donor insemination programs, adversely affects the sperm in terms of standard sperm evaluation…”
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Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay by Lee, M A, Trucco, G S, Bechtol, K B, Wummer, N, Kopf, G S, Blasco, L, Storey, B T

“…Human spermatozoa were incubated in culture medium containing human serum albumin (HSA) to promote capacitation, which was monitored by a rapid…”
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Lipid peroxidation and the reactions of superoxide and hydrogen peroxide in mouse spermatozoa by ALVAREZ, J. G, STOREY, B. T

“…Mouse spermatozoa released from the cauda epididymidis underwent spontaneous lipid peroxidation during aerobic incubation at 37 degrees C in medium containing…”
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Characterization of the mouse sperm plasma membrane zona-binding site sensitive to trypsin inhibitors by BENAU, D. A, STOREY, B. T

“…The first contact of mammalian gametes is the binding of the spermatozoon to the zona pellucida of the egg. Previous work has shown that binding of the…”
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Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay by Ward, Cynthia R., Storey, Bayard T.

“…The heads of mouse spermatozoa obtained 5 min after release from the excised caudae epididymides showed a characteristic fluorescence pattern in the presence…”
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Interactions between gametes leading to fertilization: the sperm's eye view by Storey, B T

“…Sexual reproduction requires that the gamete carrying the male-derived haploid chromatin join with the gamete carrying the female-derived haploid chromatin…”
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Assessment of the anti-microbial agent C31G as a spermicide: Comparison with nonoxynol-9 by Thompson, Kathleen A., Malamud, Daniel, Storey, Bayard T.

“…The broad-spectrum anti-microbial agent, C31G, containing an equimolar mixture of n-dodecyl-dimethylamine-Noxide (C 12-N-O) and N-(n-dodecyl),…”
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Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit spermatozoa by Alvarez, J.G, Storey, B.T

“…Damage to the plasma membrane of rabbit epididymal spermatozoa during spontaneous lipid peroxidation was examined by means of trypan blue uptake and expression…”
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A comparison of three methods for detecting the acrosome reaction in human spermatozoa by Amin, Abdel-Halim, Bailey, Janice L., Storey, Bayard T., Blasco, Luis, Heyner, Susan

“…This study was designed to compare three different fluorescent probes to assay the acrosome reaction in human spermatozoa: chlortetracycline (CTC),…”
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Evidence for plasma membrane impermeability to small ions in acrosome-intact mouse spermatozoa bound to mouse zonae pellucidae, using an aminoacridine fluorescent pH probe: time course of the zona-induced acrosome reaction monitored by both chlortetracycline and pH probe fluorescence by LEE, M. A, STOREY, B. T

“…Previous studies have shown that capacitated mouse spermatozoa bind to zonae pellucidae of mouse eggs with acrosomes apparently intact. The question addressed…”
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Mechanism of the acceleration of CO2 production from pyruvate in liver mitochondria by HCO3 by Taguchi, Y, Ono, Y, Lin, L, Storey, B T, Dodgson, S J, Forster, R E

“…To investigate the mechanism by which HCO3- accelerates pyruvate metabolism in guinea pig liver mitochondria, we measured continuously, at pH 7.4 and 37…”
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