Search Results - "Storer, A. C"

Functional expression of human cathepsin S in Saccharomyces cerevisiae. Purification and characterization of the recombinant enzyme by Brömme, D, Bonneau, P R, Lachance, P, Wiederanders, B, Kirschke, H, Peters, C, Thomas, D Y, Storer, A C, Vernet, T

“…A cDNA encoding the human lysosomal cysteine proteinase cathepsin S precursor has been expressed in yeast using the pVT100-U expression vector containing the…”
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NMR structural studies of human cystatin C dimers and monomers by Ekiel, I., Abrahamson, M., Fulton, D.B., Lindahl, P., Storer, A.C., Levadoux, W., Lafrance, M., Labelle, S., Pomerleau, Y., Groleau, D., LeSauteur, L., Gehring, K.

“…Human cystatin C undergoes dimerization before unfolding. Dimerization leads to a complete loss of its activity as a cysteine proteinase inhibitor. A similar…”
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Processing of the papain precursor: The ionization state of a conserved amino acid motif within the Pro region participates in the regulation of intramolecular processing by Vernet, T. (Institut de Biologie Structurale, Grenoble, France.), Berti, P.J, Montigny, C. de, Musil, R, Tessier, D.C, Menard, R, Magny, M.C, Storer, A.C, Thomas, D.Y

“…The cysteine protease papain is synthesized as a 40-kDa inactive precursor with a 107-amino-acid N-terminal pro region. Although sequence conservation in the…”
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Crystal structure of a papain-E-64 complex by Varughese, K. I, Ahmed, F. R, Carey, P. R, Hasnain, S, Huber, C. P, Storer, A. C

“…E-64 [1-[N-[(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucyl] amino]-4-guanidinobutane] is an irreversible inhibitor of many cysteine proteases. A papain-E-64…”
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Processing of the papain precursor. Purification of the zymogen and characterization of its mechanism of processing by Vernet, T. (National Research Council of Canada, Montreal, Quebec, Canada), Khouri, H.E, Laflamme, P, Tessier, D.C, Musil, R, Gour-Salin, B.J, Storer, A.C, Thomas, D.Y

“…The precursor of the cysteine protease papain has been expressed and secreted as propapain from insect cells infected with a recombinant baculovirus expressing…”
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Mechanistic and structural studies on Rhodococcus ATCC 39484 nitrilase by Stevenson, D E, Feng, R, Dumas, F, Groleau, D, Mihoc, A, Storer, A C

“…Rhodococcus ATCC 39484 produced a nitrilase when induced with isovaleronitrile. The enzyme was obtainable pure in milligram amounts, had a subunit Mr of 40…”
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A model to explain the pH-dependent specificity of cathepsin B-catalysed hydrolyses by KHOURI, H. E, PLOUFFE, C, HASNAIN, S, HIRAMA, T, STORER, A. C, MENARD, R

“…1. Three synthetic substrates of cathepsin B (EC 3.4.22.1) with various amino acid residues at the P2 position (Cbz-Phe-Arg-NH-Mec, Cbz-Arg-Arg-NH-Mec and…”
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Local pH-dependent conformational changes leading to proteolytic susceptibility of cystatin C by BERTI, P. J, STORER, A. C

“…Cystatin C, a cysteine protease inhibitor, was subject to hydrolysis at two sites when complexed with papain and in the presence of excess papain. A…”
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Aziridine Analogs of [[trans-(Epoxysuccinyl)-L-leucyl]amino]-4-guanidinobutane (E-64) as Inhibitors of Cysteine Proteases by Martichonok, Valeri, Plouffe, Celine, Storer, Andrew C, Menard, Robert, Jones, J. Bryan

“…Aziridine derivatives of E-64 have been synthesized, and their characterization against the cysteine proteases cathepsin B, cathepsin L, and papain is…”
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Contribution of the glutamine 19 side chain to transition-state stabilization in the oxyanion hole of papain by Menard, Robert, Carriere, Julie, Laflamme, Pierre, Plouffe, Celine, Khouri, Henri E, Vernet, Thierry, Tessier, Daniel C, Thomas, David Y, Storer, Andrew C

“…The existence of an oxyanion hole in cysteine proteases able to stabilize a transition-state complex in a manner analogous to that found with serine proteases…”
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E64 [trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane] analogues as inhibitors of cysteine proteinases : investigation of S2 subsite interactions by GOUR-SALIN, B. J, LACHANCE, P, MAGNY, M.-C, PLOUFFE, C, MENARD, R, STORER, A. C

“…A number of epoxysuccinyl amino acid benzyl esters (HO-Eps-AA-OBzl) and benzyl amides (HO-Eps-AA-NHBzl) (where AA represents amino acid) were synthesized as…”
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Alignment/Phylogeny of the Papain Superfamily of Cysteine Proteases by Berti, Paul J., Storer, Andrew C.

“…An alignment/phylogeny of the papain superfamily of cysteine proteases was created using an initial structure-based alignment followed by successive iterations…”
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Engineering of papain: selective alteration of substrate specificity by site-directed mutagenesis by Khouri, Henry E, Vernet, Thierry, Menard, Robert, Parlati, Francesco, Laflamme, Pierre, Tessier, Daniel C, Gour-Salin, Barbara, Thomas, David Y, Storer, Andrew C

“…The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis…”
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Secretion of functional papain precursor from insect cells. Requirement for N-glycosylation of the pro-region by Vernet, T, Tessier, D C, Richardson, C, Laliberté, F, Khouri, H E, Bell, A W, Storer, A C, Thomas, D Y

“…The synthetic gene coding for the precursor of the cysteine protease papain (EC 3.4.22.2) has been expressed using the baculovirus/insect cell system. The…”
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Oxyanion hole interactions in serine and cysteine proteases by Ménard, R, Storer, A C

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Benzoylamidoacetonitrile is bound as a thioimidate in the active site of papain by Brisson, J R, Carey, P R, Storer, A C

“…13C NMR spectroscopy has been used to demonstrate that 13CN-labeled benzoylamidoacetonitrile forms a covalent adduct with the thiol group of cysteine 25 in the…”
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Identification of internal autoproteolytic cleavage sites within the prosegments of recombinant procathepsin B and procathepsin S. Contribution of a plausible unimolecular autoproteolytic event for the processing of zymogens belonging to the papain family by Quraishi, O, Storer, A C

“…The steps involved in the maturation of proenzymes belonging to the papain family of cysteine proteases have been difficult to characterize. Intermolecular…”
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Catalytic mechanism in papain family of cysteine peptidases by Storer, A C, Ménard, R

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Detection of covalent enzyme-substrate complexes of nitrilase by ion-spray mass spectroscopy by Stevenson, David E., Feng, Rong, Storer, Andrew C.

“…Nitrilase from Rhodococcus ATCC 39484 was found to consist of two species of M r 40 258 ±2 and 40 388 ±2 Da. When the enzyme was incubated with nitrile…”
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Cooperativity of papain-substrate interaction energies in the S2 to S2' subsites by Berti, Paul J, Faerman, Carlos H, Storer, Andrew C

“…Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone…”
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