Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by c...

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Bibliographic Details
Published in:Conservation genetics resources Vol. 6; no. 1; pp. 185 - 187
Main Authors: Jackson, Alexis M, Munguía-Vega, Adrian, Lain, Avian S, Stokes, Simon A, Williams, Aneese J, Bernardi, Giacomo
Format: Journal Article
Language:English
Published: Dordrecht Springer-Verlag 01-03-2014
Springer Netherlands
Springer Nature B.V
Subjects:
alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
chemistry
Conservation Biology/Ecology
Ecology
Evolutionary Biology
genomic libraries
Genotyping
Heterozygosity
Life Sciences
loci
Microsatellite Letters
microsatellite repeats
Mycteroperca rosacea
Plant Genetics and Genomics
sequence analysis
Threatened species
titanium
Gulf of California
Mexico
Leopard grouper
Microsatellites
Fisheries management
Conservation
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Summary:Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.
Bibliography:http://dx.doi.org/10.1007/s12686-013-0044-0
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-013-0044-0