Search Results - "Stivers, James T"

Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation by Esadze, Alexandre, Stivers, James T

“…Preservation of the coding potential of the genome and highly regulated gene expression over the life span of a human are two fundamental requirements of life…”
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Phosphorylation of SAMHD1 Thr592 increases C-terminal domain dynamics, tetramer dissociation and ssDNA binding kinetics by Orris, Benjamin, Huynh, Kevin W, Ammirati, Mark, Han, Seungil, Bolaños, Ben, Carmody, Jason, Petroski, Matthew D, Bosbach, Benedikt, Shields, David J, Stivers, James T

“…SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) is driven into its activated tetramer form by binding of GTP activator…”
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GTP activator and dNTP substrates of HIV-1 restriction factor SAMHD1 generate a long-lived activated state by Hansen, Erik C, Seamon, Kyle J, Cravens, Shannen L, Stivers, James T

“…The HIV-1 restriction factor sterile α-motif/histidine-aspartate domain-containing protein 1 (SAMHD1) is a tetrameric protein that catalyzes the hydrolysis of…”
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Timing facilitated site transfer of an enzyme on DNA by Schonhoft, Joseph D, Stivers, James T

“…DNA repair proteins require efficient pathways to search for DNA damage lesions within a large genome. Kinetic trapping experiments define the rates of human…”
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Uracil DNA glycosylase uses DNA hopping and short-range sliding to trap extrahelical uracils by Porecha, Rishi H, Stivers, James T

“…The astonishingly efficient location and excision of damaged DNA bases by DNA repair glycosylases is an especially intriguing problem in biology. One example…”
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Single-Stranded Nucleic Acids Bind to the Tetramer Interface of SAMHD1 and Prevent Formation of the Catalytic Homotetramer by Seamon, Kyle J, Bumpus, Namandjé N, Stivers, James T

“…Sterile alpha motif and HD domain protein 1 (SAMHD1) is a unique enzyme that plays important roles in nucleic acid metabolism, viral restriction, and the…”
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Replication-competent HIV-1 in human alveolar macrophages and monocytes despite nucleotide pools with elevated dUTP by Cui, Junru, Meshesha, Mesfin, Churgulia, Natela, Merlo, Christian, Fuchs, Edward, Breakey, Jennifer, Jones, Joyce, Stivers, James T

“…Abstract Background Although CD4 + memory T cells are considered the primary latent reservoir for HIV-1, replication competent HIV has been detected in tissue…”
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Extrahelical Damaged Base Recognition by DNA Glycosylase Enzymes by Stivers, James T.

“…The efficient enzymatic detection of damaged bases concealed in the DNA double helix is an essential step during DNA repair in all cells. Emergent structural…”
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A Mechanistic Perspective on the Chemistry of DNA Repair Glycosylases by Stivers, James T, Jiang, Yu Lin

“…This review will emphasize developments since the last review of DNA glycosylases in this journal in 1998. Rather than attempting to serve as a comprehensive…”
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Deficient uracil base excision repair leads to persistent dUMP in HIV proviruses during infection of monocytes and macrophages by Meshesha, Mesfin, Esadze, Alexandre, Cui, Junru, Churgulia, Natela, Sahu, Sushil Kumar, Stivers, James T

“…Non-dividing cells of the myeloid lineage such as monocytes and macrophages are target cells of HIV that have low dNTP pool concentrations and elevated levels…”
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Deoxyguanosine-Linked Bifunctional Inhibitor of SAMHD1 dNTPase Activity and Nucleic Acid Binding by Egleston, Matthew, Dong, Linghao, Howlader, A. Hasan, Bhat, Shridhar, Orris, Benjamin, Bianchet, Mario A., Greenberg, Marc M., Stivers, James T.

“…Sterile alpha motif histidine-aspartate domain protein 1 (SAMHD1) is a deoxynucleotide triphosphohydrolase that exists in monomeric, dimeric, and tetrameric…”
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Dynamics of Uracil and 5-Fluorouracil in DNA by Parker, Jared B, Stivers, James T

“…The prodrug 5-fluorouracil (5-FU), after activation into 5-F-dUMP, is an extensively used anticancer agent that inhibits thymidylate synthase and leads to…”
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Uracil DNA glycosylase initiates degradation of HIV-1 cDNA containing misincorporated dUTP and prevents viral integration by Weil, Amy F, Ghosh, Devlina, Zhou, Yan, Seiple, Lauren, McMahon, Moira A, Spivak, Adam M, Siliciano, Robert F, Stivers, James T

“…HIV-1 reverse transcriptase discriminates poorly between dUTP and dTTP, and accordingly, viral DNA products become heavily uracilated when viruses infect host…”
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A Portable Hot Spot Recognition Loop Transfers Sequence Preferences from APOBEC Family Members to Activation-induced Cytidine Deaminase by Kohli, Rahul M., Abrams, Shaun R., Gajula, Kiran S., Maul, Robert W., Gearhart, Patricia J., Stivers, James T.

“…Enzymes of the AID/APOBEC family, characterized by the targeted deamination of cytosine to generate uracil within DNA, mediate numerous critical immune…”
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Microscopic mechanism of DNA damage searching by hOGG1 by Rowland, Meng M, Schonhoft, Joseph D, McKibbin, Paige L, David, Sheila S, Stivers, James T

“…The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed…”
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Enzymatic capture of an extrahelical thymine in the search for uracil in DNA by Stivers, James T, Parker, Jared B, Bianchet, Mario A, Krosky, Daniel J, Friedman, Joshua I, Amzel, L. Mario

“…The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of…”
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Effect of the Thymidylate Synthase Inhibitors on dUTP and TTP Pool Levels and the Activities of DNA Repair Glycosylases on Uracil and 5-Fluorouracil in DNA by Grogan, Breeana C, Parker, Jared B, Guminski, Amy F, Stivers, James T

“…5-Fluorouracil (5-FU), 5-fluorodeoxyuridine (5-dUrd), and raltitrixed (RTX) are anticancer agents that target thymidylate synthase (TS), thereby blocking the…”
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Impact of linker strain and flexibility in the design of a fragment-based inhibitor by Stivers, James T, Chung, Suhman, Parker, Jared B, Bianchet, Mario, Amzel, L Mario

“…The linking together of molecular fragments that bind to adjacent sites on an enzyme can lead to high-affinity inhibitors. Ideally, this strategy would use…”
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AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation by Nabel, Christopher S, Jia, Huijue, Ye, Yu, Shen, Li, Goldschmidt, Hana L, Stivers, James T, Zhang, Yi, Kohli, Rahul M

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A Transient Kinetic Analysis of PRMT1 Catalysis by Feng, You, Xie, Nan, Jin, Miyeong, Stahley, Mary R, Stivers, James T, Zheng, Yujun George

“…Post-translational modifications (PTMs) are important strategies used by eukaryotic organisms to modulate their phenotypes. One of the well-studied PTMs,…”
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