Search Results - "Stiffler, Michael A"

Evolvability as a Function of Purifying Selection in TEM-1 β-Lactamase by Stiffler, Michael A., Hekstra, Doeke R., Ranganathan, Rama

“…Evolvability—the capacity to generate beneficial heritable variation—is a central property of biological systems. However, its origins and modulation by…”
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Inferring protein 3D structure from deep mutation scans by Rollins, Nathan J., Brock, Kelly P., Poelwijk, Frank J., Stiffler, Michael A., Gauthier, Nicholas P., Sander, Chris, Marks, Debora S.

“…We describe an experimental method of three-dimensional (3D) structure determination that exploits the increasing ease of high-throughput mutational scans…”
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PDZ Domain Binding Selectivity Is Optimized Across the Mouse Proteome by Stiffler, Michael A, Chen, Jiunn R, Grantcharova, Viara P, Lei, Ying, Fuchs, Daniel, Allen, John E, Zaslavskaia, Lioudmila A, MacBeath, Gavin

“…PDZ domains have long been thought to cluster into discrete functional classes defined by their peptide-binding preferences. We used protein microarrays and…”
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Simultaneous enhancement of multiple functional properties using evolution-informed protein design by Fram, Benjamin, Su, Yang, Truebridge, Ian, Riesselman, Adam J., Ingraham, John B., Passera, Alessandro, Napier, Eve, Thadani, Nicole N., Lim, Samuel, Roberts, Kristen, Kaur, Gurleen, Stiffler, Michael A., Marks, Debora S., Bahl, Christopher D., Khan, Amir R., Sander, Chris, Gauthier, Nicholas P.

“…A major challenge in protein design is to augment existing functional proteins with multiple property enhancements. Altering several properties likely…”
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Necl‐4/Cadm4 recruits Par‐3 to the Schwann cell adaxonal membrane by Meng, Xiaosong, Maurel, Patrice, Lam, Isabel, Heffernan, Corey, Stiffler, Michael A., McBeath, Gavin, Salzer, James L.

“…Interactions between axons and Schwann cells are essential for the acquisition of Schwann cell radial and longitudinal polarity and myelin sheath assembly. In…”
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Predicting PDZ domain-peptide interactions from primary sequences by MacBeath, Gavin, Chen, Jiunn R, Chang, Bryan H, Allen, John E, Stiffler, Michael A

“…PDZ domains constitute one of the largest families of interaction domains and function by binding the C termini of their target proteins. Using Bayesian…”
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Quantifying protein-protein interactions in high throughput using protein domain microarrays by MacBeath, Gavin, Kaushansky, Alexis, Allen, John E, Gordus, Andrew, Stiffler, Michael A, Karp, Ethan S, Chang, Bryan H

“…Protein microarrays provide an efficient way to identify and quantify protein–protein interactions in high throughput. One drawback of this technique is that…”
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Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors by Chen, Wen-Yi, Singh, Deepali, Lai, Lien B, Stiffler, Michael A, Lai, Hue D, Foster, Mark P, Gopalan, Venkat

“…RNase P, which catalyzes tRNA 5'-maturation, typically comprises a catalytic RNase P RNA (RPR) and a varying number of RNase P proteins (RPPs): 1 in bacteria,…”
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing by Stiffler, Michael A, Subramanian, Subu K, Salinas, Victor H, Ranganathan, Rama

“…Site-directed mutagenesis has long been used as a method to interrogate protein structure, function and evolution. Recent advances in massively-parallel…”
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Uncovering Quantitative Protein Interaction Networks for Mouse PDZ Domains Using Protein Microarrays by Stiffler, Michael A, Grantcharova, Viara P, Sevecka, Mark, MacBeath, Gavin

“…One of the principal challenges in systems biology is to uncover the networks of protein−protein interactions that underlie most biological processes. To date,…”
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Corrigendum: Predicting PDZ domain-peptide interactions from primary sequences by Chen, Jiunn R, Chang, Bryan H, Allen, John E, Stiffler, Michael A, MacBeath, Gavin

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Erratum: Corrigendum: Predicting PDZ domain–peptide interactions from primary sequences by Chen, Jiunn R, Chang, Bryan H, Allen, John E, Stiffler, Michael A, MacBeath, Gavin

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Correction: Corrigendum: Predicting PDZ domain–peptide interactions from primary sequences by Chen, Jiunn R, Chang, Bryan H, Allen, John E, Stiffler, Michael A, MacBeath, Gavin

“…Nat. Biotechnol. 26, 1041–1045 (2008); published online 17 August 2008; corrected after print 9 October 2008 In the version of this article initially…”
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