Search Results - "Steward, Lance E."

Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A) by Jacky, Birgitte P S, Garay, Patton E, Dupuy, Jérôme, Nelson, Jeremy B, Cai, Brian, Molina, Yanira, Wang, Joanne, Steward, Lance E, Broide, Ron S, Francis, Joseph, Aoki, K Roger, Stevens, Raymond C, Fernández-Salas, Ester

“…Botulinum neurotoxin serotype A (BoNT/A) causes transient muscle paralysis by entering motor nerve terminals (MNTs) where it cleaves the SNARE protein…”
Get full text

Reengineering the specificity of the highly selective Clostridium botulinum protease via directed evolution by Dyer, Rebekah P., Isoda, Hariny M., Salcedo, Gabriela S., Speciale, Gaetano, Fletcher, Madison H., Le, Linh Q., Liu, Yi, Brami-Cherrier, Karen, Malik, Shiazah Z., Vazquez-Cintron, Edwin J., Chu, Andrew C., Rupp, David C., Jacky, Birgitte P. S., Nguyen, Thu T. M., Katz, Benjamin B., Steward, Lance E., Majumdar, Sudipta, Brideau-Andersen, Amy D., Weiss, Gregory A.

“…The botulinum neurotoxin serotype A (BoNT/A) cuts a single peptide bond in SNAP25, an activity used to treat a wide range of diseases. Reengineering the…”
Get full text

Novel Chimeras of Botulinum Neurotoxins A and E Unveil Contributions from the Binding, Translocation, and Protease Domains to Their Functional Characteristics by Wang, Jiafu, Meng, Jianghui, Lawrence, Gary W., Zurawski, Tomas H., Sasse, Astrid, Bodeker, MacDara O., Gilmore, Marcella A., Fernández-Salas, Ester, Francis, Joseph, Steward, Lance E., Aoki, K. Roger, Dolly, J. Oliver

“…Hyperexcitability disorders of cholinergically innervated muscles are treatable with botulinum neurotoxin (BoNT) A. The seven serotypes (A–G) potently block…”
Get full text

OnabotulinumtoxinA Displays Greater Biological Activity Compared to IncobotulinumtoxinA, Demonstrating Non-Interchangeability in Both In Vitro and In Vivo Assays by Rupp, David, Nicholson, Greg, Canty, David, Wang, Joanne, Rhéaume, Catherine, Le, Linh, Steward, Lance E, Washburn, Mark, Jacky, Birgitte P, Broide, Ron S, Philipp-Dormston, Wolfgang G, Brin, Mitchell F, Brideau-Andersen, Amy

“…Differences in botulinum neurotoxin manufacturing, formulation, and potency evaluation can impact dose and biological activity, which ultimately affect…”
Get full text

Plasma Membrane Localization Signals in the Light Chain of Botulinum Neurotoxin by Fernández-Salas, Ester, Steward, Lance E., Ho, Helen, Garay, Patton E., Sun, Sarah W., Gilmore, Marcella A., Ordas, Joseph V., Wang, Joanne, Francis, Joseph, Aoki, K. Roger, Boyer, Herbert W.

“…Botulinum neurotoxin (BoNT) is a potent biological substance used to treat neuromuscular and pain disorders. Both BoNT type A and BoNT type E display…”
Get full text

Is the light chain subcellular localization an important factor in botulinum toxin duration of action? by Fernández-Salas, Ester, Ho, Helen, Garay, Patton, Steward, Lance E., Aoki, K. Roger

“…Botulinum neurotoxins (BoNT) are therapeutic proteins that are specific, potent, and effective. They are highly specific in binding to motor neurons but do not…”
Get full text

Location of the Synaptosome-Binding Regions on Botulinum Neurotoxin B by Dolimbek, Behzod Z, Steward, Lance E, Aoki, K. Roger, Atassi, M. Zouhair

“…The regions of botulinum neurotoxin B (BoNT/B) involved in binding to mouse brain synaptosomes (snps) were localized. Sixty 19-residue overlapping peptides…”
Get full text

Regions of botulinum neurotoxin A light chain recognized by human anti-toxin antibodies from cervical dystonia patients immunoresistant to toxin treatment. The antigenic structure of the active toxin recognized by human antibodies by Atassi, M. Zouhair, Dolimbek, Behzod Z, Jankovic, Joseph, Steward, Lance E, Aoki, K. Roger

“…Abstract This work was aimed at determining the BoNT/A L-chain antigenic regions recognized by blocking antibodies in human antisera from cervical dystonia…”
Get full text

Mapping of the regions on the heavy chain of botulinum neurotoxin A (BoNT/A) recognized by antibodies of cervical dystonia patients with immunoresistance to BoNT/A by Dolimbek, Behzod Z., Aoki, K. Roger, Steward, Lance E., Jankovic, Joseph, Atassi, M. Zouhair

“…The purpose of this work was to map the entire recognition profile of the H chain of botulinum neurotoxin A (BoNT/A) by Abs in sera that have protective…”
Get full text

Depolarization after resonance energy transfer (DARET): A sensitive fluorescence-based assay for botulinum neurotoxin protease activity by Gilmore, Marcella A., Williams, Dudley, Okawa, Yumiko, Holguin, Bret, James, Nicholas G., Ross, Justin A., Roger Aoki, K., Jameson, David M., Steward, Lance E.

“…The DARET (depolarization after resonance energy transfer) assay is a coupled Förster resonance energy transfer (FRET)–fluorescence polarization assay for…”
Get full text

Synaptotagmin II and Gangliosides Bind Independently with Botulinum Neurotoxin B but Each Restrains the Other by Atassi, M. Zouhair, Taruishi, Midori, Naqvi, Masooma, Steward, Lance E., Aoki, K. Roger

“…Botulinum neurotoxin type B (BoNT/B) initiates its toxicity by binding to synaptotagmin II (SytII) and gangliosides GD1a and GT1b on the neural membrane. We…”
Get full text

Molecular immune recognition of botulinum neurotoxin B. The light chain regions that bind human blocking antibodies from toxin-treated cervical dystonia patients. Antigenic structure of the entire BoNT/B molecule by Atassi, M. Zouhair, Jankovic, Joseph, Steward, Lance E, Aoki, K. Roger, Dolimbek, Behzod Z

“…Abstract We recently mapped the regions on the heavy (H) chain of botulinum neurotoxin, type B (BoNT/B) recognized by blocking antibodies (Abs) from cervical…”
Get full text

Characterization of Förster resonance energy transfer in a botulinum neurotoxin protease assay by Ross, Justin A., Gilmore, Marcella A., Williams, Dudley, Aoki, K. Roger, Steward, Lance E., Jameson, David M.

“…Our previous article described a fluorescence-based assay for monitoring the proteolytic activity of botulinum neurotoxin types A and E (BoNT/A and BoNT/E). As…”
Get full text

Regions of recognition by blocking antibodies on the light chain of botulinum neurotoxin A: antigenic structure of the entire toxin by Dolimbek, Behzod Z, Steward, Lance E, Aoki, K Roger, Atassi, M Zouhair

“…The continuous regions on botulinum neurotoxin A (BoNT/A) light (L) chain recognized by anti-toxin antibodies (Abs) from mouse, horse and chicken have been…”
Get full text

Molecular recognition of botulinum neurotoxin B heavy chain by human antibodies from cervical dystonia patients that develop immunoresistance to toxin treatment by Atassi, M. Zouhair, Dolimbek, Behzod Z., Jankovic, Joseph, Steward, Lance E., Aoki, K. Roger

“…We determined the entire profile of the continuous antigenic regions recognized by blocking antibodies (Abs) in sera from 30 BoNT/B-treated cervical dystonia…”
Get full text

Antibodies against a synthetic peptide designed to mimic a surface area of the H chain of botulinum neurotoxin A by Atassi, M. Zouhair, Dolimbek, Behzod Z, Steward, Lance E, Aoki, K. Roger

“…Abstract A surface-simulation peptide, SQMIN [GG] TTNI [G] NSIS [G] RDTH [G] NLES , (SS-peptide) was synthesized that described the spatial interrelationships…”
Get full text

Immune recognition of botulinum neurotoxin B: Antibody-binding regions on the heavy chain of the toxin by Dolimbek, Behzod Z., Steward, Lance E., Aoki, K. Roger, Atassi, M. Zouhair

“…The purpose of this work was to map the continuous regions recognized by human, horse and mouse anti-botulinum neurotoxin B (BoNT/B) antibodies (Abs). We…”
Get full text

Regions of recognition by blocking antibodies on the light chain of botulinum neurotoxin A by Dolimbek, Behzod Z, Steward, Lance E, Aoki, K. Roger, Atassi, M. Zouhair

“…Abstract The continuous regions on botulinum neurotoxin A (BoNT/A) light (L) chain recognized by anti-toxin antibodies (Abs) from mouse, horse and chicken have…”
Get full text

The binding sites on botulinum neurotoxin B for synaptotagmin and for blocking antibodies by Dolimbek, Behzod Z., Steward, Lance E., Aoki, K. Roger, Atassi, M. Zouhair

Get full text

Inhibition of Botulinum Neurotoxin A Toxic Action In Vivo by Synthetic Synaptosome- and Blocking Antibody-Binding Regions by Atassi, M. Zouhair, Dolimbek, Behzod Z., Steward, Lance E., Aoki, K. Roger

“…In previous studies, we showed that certain peptides of the H N and H C domains of the H-chain of BoNT/A bind to mouse brain synaptosomes (snps). There was…”
Get full text