Search Results - "Stevenson, Tracy I"

Mass Spectrometry of Proteins Directly from Polyacrylamide Gels by Ogorzalek Loo, Rachel R, Stevenson, Tracy I, Mitchell, Charles, Loo, Joseph A, Andrews, Philip C

“…The direct combination of thin-layer gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry has been demonstrated with good…”
Get full text

Large-scale evaluation of quantitative reproducibility and proteome coverage using acid cleavable isotope coded affinity tag mass spectrometry for proteomic profiling by Molloy, Mark P., Donohoe, Sam, Brzezinski, Erin E., Kilby, Greg W., Stevenson, Tracy I., Baker, J. David, Goodlett, David R., Gage, Douglas A.

“…Strategies employing non‐gel based methods for quantitative proteomic profiling such as isotope coded affinity tags coupled with mass spectrometry (ICAT‐MS)…”
Get full text

Proteomic analysis of hyperdynamic mouse hearts with enhanced sarcoplasmic reticulum calcium cycling by Chu, Guoxiang, Kerr, Jaclyn P., Mitton, Bryan, Egnaczyk, Gregory F., Vazquez, Jenny A., Shen, Meilan, Kilby, Greg W., Stevenson, Tracy I., Maggio, John E., Vockley, Jerry, Rapundalo, Stephen T., Kranias, Evangelia G.

“…ABSTRACT Depressed sarcoplasmic reticulum (SR) Ca‐cycling is a hallmark of human and experimental heart failure. Strategies to improve this impairment by…”
Get full text

Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: implications for proteome mapping by Ogorzalek Loo, R R, Mitchell, C, Stevenson, T I, Martin, S A, Hines, W M, Juhasz, P, Patterson, D H, Peltier, J M, Loo, J A, Andrews, P C

“…Matrix-assisted laser desorption ionization (MALDI) mass spectra have been obtained directly from thin-layer isoelectric focusing (IEF) gels with as little as…”
Get more information

Coupling Capillary High-Performance Liquid Chromatography to Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and N-Terminal Sequencing of Peptides via Automated Microblotting onto Membrane Substrates by Stevenson, Tracy I., Loo, Joseph A., Greis, Kenneth D.

“…To minimize low-quantity sample handling for protein sequencing and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a system consisting…”
Get full text

Application of electrospray ionization mass spectrometry for studying human immunodeficiency virus protein complexes by Loo, Joseph A., Holler, Tod P., Foltin, Susan K., McConnell, Patrick, Banotai, Craig A., Horne, Nicole M., Mueller, W. Tom, Stevenson, Tracy I., Mack, David P.

“…Mass spectrometry (MS) with electrospray ionization (ESI) has shown utility for studying noncovalent protein complexes, as it offers advantages in sensitivity,…”
Get full text

Cardiac sarcoplasmic reticulum and sarcolemmal proteins separated by two-dimensional electrophoresis: Surfactant effects on membrane solubilization by Macri, Joseph, McGee, Beth, Thomas, John N., Du, Ping, Stevenson, Tracy I., Kilby, Greg W., Rapundalo, Stephen T.

“…We evaluated the use of the alkyaryl amidosulfobetaine zwitterionic detergent, designated as C8∅︁, to facilitate the solubilization of cardiac subcellular,…”
Get full text

Application of mass spectrometry for target identification and characterization by Loo, Joseph A., DeJohn, Dana E., Du, Ping, Stevenson, Tracy I., Ogorzalek Loo, Rachel R.

“…Mass spectrometry‐based methodologies span the vast expanse of drug discovery. Both electrospray ionization (ESI) and matrix‐assisted laser…”
Get full text

Diffusive transfer to membranes as an effective interface between gel electrophoresis and mass spectrometry by Ogorzalek Loo, Rachel R., Mitchell, Charles, Stevenson, Tracy I., Loo, Joseph A., Andrews, Philip C.

“…Diffusive transfer was examined as a blotting method to transfer proteins from polyacrylamide gels to membranes for ultraviolet matrix-assisted laser…”
Get full text