Search Results - "Sterling, Harry"

Electrothermal Supercharging of Proteins in Native Electrospray Ionization by Sterling, Harry J, Cassou, Catherine A, Susa, Anna C, Williams, Evan R

“…The formation of high charge-state protein ions with nanoelectrospray ionization (nESI) from purely aqueous ammonium bicarbonate solutions at neutral pH, where…”
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Real-Time Hydrogen/Deuterium Exchange Kinetics via Supercharged Electrospray Ionization Tandem Mass Spectrometry by Sterling, Harry J, Williams, Evan R

“…Amide hydrogen/deuterium exchange (HDX) rate constants of bovine ubiquitin in an ammonium acetate solution containing 1% of the electrospray ionization (ESI)…”
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Protein Conformation and Supercharging with DMSO from Aqueous Solution by Sterling, Harry J., Prell, James S., Cassou, Catherine A., Williams, Evan R.

“…The efficacy of dimethyl sulfoxide (DMSO) as a supercharging reagent for protein ions formed by electrospray ionization from aqueous solution and the mechanism…”
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Origin of Supercharging in Electrospray Ionization of Noncovalent Complexes from Aqueous Solution by Sterling, Harry J., Williams, Evan R.

“…The use of m-nitrobenzyl alcohol (m-NBA) to enhance charging of noncovalent complexes formed by electrospray ionization from aqueous solutions was…”
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Electrothermal Supercharging in Mass Spectrometry and Tandem Mass Spectrometry of Native Proteins by Cassou, Catherine A, Sterling, Harry J, Susa, Anna C, Williams, Evan R

“…Electrothermal supercharging of protein ions formed by electrospray ionization from buffered aqueous solutions results in significant increases to both the…”
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The Protective Antigen Component of Anthrax Toxin Forms Functional Octameric Complexes by Kintzer, Alexander F., Thoren, Katie L., Sterling, Harry J., Dong, Ken C., Feld, Geoffrey K., Tang, Iok I., Zhang, Teri T., Williams, Evan R., Berger, James M., Krantz, Bryan A.

“…The assembly of bacterial toxins and virulence factors is critical to their function, but the regulation of assembly during infection has not been studied. We…”
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Tpr directly binds to Mad1 and Mad2 and is important for the Mad1-Mad2-mediated mitotic spindle checkpoint by Lee, Sang Hyun, Sterling, Harry, Burlingame, Alma, McCormick, Frank

“…The mitotic arrest-deficient protein Mad1 forms a complex with Mad2, which is required for imposing mitotic arrest on cells in which the spindle assembly is…”
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Structural basis for the unfolding of anthrax lethal factor by protective antigen oligomers by Tang, Iok I, Thoren, Katie L, Feld, Geoffrey K, Greenberg, Shoshana G, Krantz, Bryan A, Williams, Evan R, Sterling, Harry J, Kintzer, Alexander F

“…The anthrax lethal toxin protein transporter consists of protective antigen (PA) and lethal factor (LF), with LF unfolding for translocation into the host…”
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Supercharging Protein Complexes from Aqueous Solution Disrupts their Native Conformations by Sterling, Harry J., Kintzer, Alexander F., Feld, Geoffrey K., Cassou, Catherine A., Krantz, Bryan A., Williams, Evan R.

“…The effects of aqueous solution supercharging on the solution- and gas-phase structures of two protein complexes were investigated using traveling-wave ion…”
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Anthrax toxin receptor drives protective antigen oligomerization and stabilizes the heptameric and octameric oligomer by a similar mechanism by Kintzer, Alexander F, Sterling, Harry J, Tang, Iok I, Williams, Evan R, Krantz, Bryan A

“…Anthrax toxin is comprised of protective antigen (PA), lethal factor (LF), and edema factor (EF). These proteins are individually nontoxic; however, when PA…”
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The role of conformational flexibility on protein supercharging in native electrospray ionization by Sterling, Harry J, Cassou, Catherine A, Trnka, Michael J, Burlingame, A L, Krantz, Bryan A, Williams, Evan R

“…Effects of covalent intramolecular bonds, either native disulfide bridges or chemical crosslinks, on ESI supercharging of proteins from aqueous solutions were…”
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Effects of Supercharging Reagents on Noncovalent Complex Structure in Electrospray Ionization from Aqueous Solutions by Sterling, Harry J., Daly, Michael P., Feld, Geoffrey K., Thoren, Katie L., Kintzer, Alexander F., Krantz, Bryan A., Williams, Evan R.

“…The effects of two supercharging reagents, m-nitrobenzyl alcohol ( m-NBA) and sulfolane, on the charge-state distributions and conformations of myoglobin ions…”
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Effects of Buffer Loading for Electrospray Ionization Mass Spectrometry of a Noncovalent Protein Complex that Requires High Concentrations of Essential Salts by Sterling, Harry J., Batchelor, Joseph D., Wemmer, David E., Williams, Evan R.

“…Electrospray ionization (ESI) mass spectrometry (MS) is a powerful method for analyzing the active forms of macromolecular complexes of biomolecules. However,…”
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Role of the Protective Antigen Octamer in the Molecular Mechanism of Anthrax Lethal Toxin Stabilization in Plasma by Kintzer, Alexander F., Sterling, Harry J., Tang, Iok I., Abdul-Gader, Ali, Miles, Andrew J., Wallace, B.A., Williams, Evan R., Krantz, Bryan A.

“…Anthrax is caused by strains of Bacillus anthracis that produce two key virulence factors, anthrax toxin (Atx) and a poly-γ-D-glutamic acid capsule. Atx is…”
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Biophysical Mass Spectrometry Techniques for Probing the Higher-Order Structure of Proteins and Complexes by Sterling, Harry John

“…Electrospray ionization mass spectrometry (ESI-MS) is a powerful analytical platform for answering a wide variety of questions about the identity, quantity,…”
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Intracerebroventricular enzyme replacement therapy with β-galactosidase reverses brain pathologies due to GM1 gangliosidosis in mice by Chen, Joseph C., Luu, Amanda R., Wise, Nathan, Angelis, Rolando De, Agrawal, Vishal, Mangini, Linley, Vincelette, Jon, Handyside, Britta, Sterling, Harry, Lo, Melanie J., Wong, Hio, Galicia, Nicole, Pacheco, Glenn, Van Vleet, Jeremy, Giaramita, Alexander, Fong, Sylvia, Roy, Sushmita M., Hague, Chuck, Lawrence, Roger, Bullens, Sherry, Christianson, Terri M., d'Azzo, Alessandra, Crawford, Brett E., Bunting, Stuart, LeBowitz, Jonathan H., Yogalingam, Gouri

“…Autosomal recessive mutations in the galactosidase β1 (GLB1) gene cause lysosomal β-gal deficiency, resulting in accumulation of galactose-containing…”
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Proteome profiling and vector yield optimization in a recombinant adeno‐associated virus‐producing yeast model by Aponte‐Ubillus, Juan Jose, Barajas, Daniel, Sterling, Harry, Aghajanirefah, Ali, Bardliving, Cameron, Peltier, Joseph, Shamlou, Parviz, Roy, Mimi, Gold, Daniel

“…ABSTRACT Recent studies on recombinant adeno‐associated viral (rAAV) vector production demonstrated the generation of infectious viral particles in…”
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Beyond the God Delusion: How Radical Theology Harmonizes Science and Religion - By Richard Grigg by Sterling, Harry

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Receiver Domains Control the Active-State Stoichiometry of Aquifex aeolicus σ54 Activator NtrC4, as Revealed by Electrospray Ionization Mass Spectrometry by Batchelor, Joseph D., Sterling, Harry J., Hong, Eunmi, Williams, Evan R., Wemmer, David E.

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Receiver domains control the active state stoichiometry of Aquifex aeolicus σ54 activator NtrC4, as revealed by electrospray mass spectrometry by Batchelor, Joseph D., Sterling, Harry J., Hong, Eunmi, Williams, Evan R., Wemmer, David E.

“…A common challenge with studies of proteins in vitro is determining which constructs and conditions are most physiologically relevant. σ 54 activators are…”
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