Kinetic constraints and features imposed by the immobilization of enzymes onto solid matrices: a key to advanced biotransformation

The kinetics of immobilized enzymes can not be analyzed by means of the simple Michaelis-Menten concept, which generally fails to describe the immobilized state due to both its probable barriers, and because the active concentration of the enzyme approaches, or even exceeds this of its substrate(s)....

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Bibliographic Details
Published in:	Indian journal of experimental biology Vol. 52; no. 11; pp. 1045 - 1051
Main Authors:	Foukis, A, Stergiou, P Y, Filippou, M, Koukouritaki, M, Parapouli, M, Theodorou, L G, Hatziloukas, E, Afendra, A, Pandey, A, Papamichael, E M
Format:	Journal Article
Language:	English
Published:	India 01-11-2014
Subjects:	Biotransformation Catalysis Enzymes, Immobilized - metabolism Kinetics Mathematics Solvents
Online Access:	Get full text
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Summary:	The kinetics of immobilized enzymes can not be analyzed by means of the simple Michaelis-Menten concept, which generally fails to describe the immobilized state due to both its probable barriers, and because the active concentration of the enzyme approaches, or even exceeds this of its substrate(s). In such cases, the various experimental data are usually treated by complex rate equations comprising too many parameters acquiring different natures and meanings, depending on both the properties of the immobilization state and the experimental conditions; thus, more likely, only apparent values of the Michaelis-Menten kinetic parameters can be estimated experimentally. Likewise, immobilization is often a key method in optimizing the operational performance of enzymes, in both laboratory and industrial scale, and affects considerably the kinetics in non-aqueous and non-conventional media due to several issues as the structural changes of the enzyme molecule, the heterogeneity of the system, and the partial or total absence of water. In this work a theoretical approach is described on the formulation of simplified rate equations, reflecting also the actual mass balances of the reactants, in the case where esterification synthetic reactions are catalyzed by immobilized lipases, in either a non-aqueous organic solvent or in a non-solvent system.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0019-5189