Search Results - "Stepien, P"

Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP by Stepien, Karolina P., Prinslow, Eric A., Rizo, Josep

“…Munc18-1 and Munc13-1 orchestrate assembly of the SNARE complex formed by syntaxin-1, SNAP-25 and synaptobrevin, allowing exquisite regulation of…”
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Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci by Borowski, Lukasz S, Dziembowski, Andrzej, Hejnowicz, Monika S, Stepien, Piotr P, Szczesny, Roman J

“…RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria…”
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Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria by Pietras, Zbigniew, Wojcik, Magdalena A., Borowski, Lukasz S., Szewczyk, Maciej, Kulinski, Tomasz M., Cysewski, Dominik, Stepien, Piotr P., Dziembowski, Andrzej, Szczesny, Roman J.

“…The GC skew in vertebrate mitochondrial genomes results in synthesis of RNAs that are prone to form G-quadruplexes (G4s). Such RNAs, although mostly…”
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Mitochondrial biogenesis and neural differentiation of human iPSC is modulated by idebenone in a developmental stage-dependent manner by Augustyniak, J., Lenart, J., Zychowicz, M., Stepien, P. P., Buzanska, L.

“…Idebenone, the synthetic analog of coenzyme Q10 can improve electron transport in mitochondria. Therefore, it is used in the treatment of Alzheimer’s disease…”
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Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and αSNAP by Prinslow, Eric A, Stepien, Karolina P, Pan, Yun-Zu, Xu, Junjie, Rizo, Josep

“…Neurotransmitter release requires formation of trans-SNARE complexes between the synaptic vesicle and plasma membranes, which likely underlies synaptic vesicle…”
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Reference Gene Validation via RT–qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors by Augustyniak, Justyna, Lenart, Jacek, Lipka, Gabriela, Stepien, Piotr P., Buzanska, Leonora

“…Correct selection of the reference gene(s) is the most important step in gene expression analysis. The aims of this study were to identify and evaluate the…”
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Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans by Tien, Chi-Wei, Yu, Bin, Huang, Mengjia, Stepien, Karolina P., Sugita, Kyoko, Xie, Xiaoyu, Han, Liping, Monnier, Philippe P., Zhen, Mei, Rizo, Josep, Gao, Shangbang, Sugita, Shuzo

“…Assembly of SNARE complexes that mediate neurotransmitter release requires opening of a ‘closed’ conformation of UNC-64/syntaxin. Rescue of unc-13/Munc13…”
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Molecular machinery turns full circle by Rizo, Josep, Jaczynska, Klaudia, Stepien, Karolina P

“…Two proteins called Sec17 and Sec18 may have a larger role in membrane fusion than is commonly assumed in textbook models…”
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Sensitivity of hiPSC-derived neural stem cells (NSC) to Pyrroloquinoline quinone depends on their developmental stage by Augustyniak, J., Lenart, J., Zychowicz, M., Lipka, G., Gaj, P., Kolanowska, M., Stepien, P.P., Buzanska, L.

“…Pyrroloquinoline quinone (PQQ) is a factor influencing on the mitochondrial biogenesis. In this study the PQQ effect on viability, total cell number,…”
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Human mitochondrial RNA turnover caught in flagranti: involvement of hSuv3p helicase in RNA surveillance by Szczesny, Roman J, Borowski, Lukasz S, Brzezniak, Lien K, Dmochowska, Aleksandra, Gewartowski, Kamil, Bartnik, Ewa, Stepien, Piotr P

“…The mechanism of human mitochondrial RNA turnover and surveillance is still a matter of debate. We have obtained a cellular model for studying the role of…”
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Synaptotagmin-1–, Munc18-1–, and Munc13-1–dependent liposome fusion with a few neuronal SNAREs by Stepien, Karolina P., Rizo, Josep

“…Neurotransmitter release is governed by eight central proteins among other factors: the neuronal SNAREs syntaxin-1, synaptobrevin, and SNAP-25, which form a…”
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The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism by Dziembowski, Andrzej, Piwowarski, Jan, Hoser, Rafal, Minczuk, Michal, Dmochowska, Aleksandra, Siep, Michel, van der Spek, Hans, Grivell, Les, Stepien, Piotr P

“…The yeast mitochondrial degradosome (mtEXO) is an NTP-dependent exoribonuclease involved in mitochondrial RNA metabolism. Previous purifications suggested that…”
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Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs by Brzezniak, Lien K., Bijata, Monika, Szczesny, Roman J., Stepien, Piotr P.

“…Accurate tRNA processing is crucial for human mitochondrial genome expression, but the mechanisms of mt-tRNA cleavage and the key enzymes involved in this…”
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Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase by Tomecki, Rafal, Dmochowska, Aleksandra, Gewartowski, Kamil, Dziembowski, Andrzej, Stepien, Piotr P.

“…We report here on the identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. Immunocytochemical experiments confirm that the enzyme…”
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In vitro Reconstitution and Characterization of the Yeast Mitochondrial Degradosome Complex Unravels Tight Functional Interdependence by Malecki, Michal, Jedrzejczak, Robert, Stepien, Piotr P., Golik, Pawel

“…The mitochondrial degradosome (mtEXO), the main RNA-degrading complex of yeast mitochondria, is composed of two subunits: an exoribonuclease encoded by the…”
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Human Polynucleotide Phosphorylase, hPNPase, is Localized in Mitochondria by Piwowarski, Jan, Grzechnik, Pawel, Dziembowski, Andrzej, Dmochowska, Aleksandra, Minczuk, Michal, Stepien, Piotr P.

“…The human gene encoding a polynucleotide phosphorylase (hPNPase) has been recently identified as strongly up-regulated in two processes leading to irreversible…”
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Controlling the mitochondrial antisense - role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance by Pietras, Zbigniew, Wojcik, Magdalena A., Borowski, Lukasz S., Szewczyk, Maciej, Kulinski, Tomasz M., Cysewski, Dominik, Stepien, Piotr P., Dziembowski, Andrzej, Szczesny, Roman J.

“…Transcription of the human mitochondrial genome produces a vast amount of non-coding antisense RNAs. These RNA species can form G-quadraplexes (G4), which…”
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Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA by Minczuk, Michal, Piwowarski, Jan, Papworth, Monika A., Awiszus, Karen, Schalinski, Sarah, Dziembowski, Andrzej, Dmochowska, Aleksandra, Bartnik, Ewa, Tokatlidis, Kostas, Stepien, Piotr P., Borowski, Peter

“…We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the…”
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Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress by Stepien, P, Klbus, G

“…Hydroponically grown cucumber plants were exposed to 14-d period of salinity (0, 50, 100 mM NaCl). NaCl caused reduction in the relative water content in the…”
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Re‐examining how Munc13‐1 facilitates opening of syntaxin‐1 by Magdziarek, Magdalena, Bolembach, Agnieszka A., Stepien, Karolina P., Quade, Bradley, Liu, Xiaoxia, Rizo, Josep

“…Munc13‐1 is crucial for neurotransmitter release and, together with Munc18‐1, orchestrates assembly of the neuronal SNARE complex formed by syntaxin‐1,…”
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