Search Results - "Steinmetz, Eric J."

Genome-Wide Distribution of Yeast RNA Polymerase II and Its Control by Sen1 Helicase by Steinmetz, Eric J., Warren, Christopher L., Kuehner, Jason N., Panbehi, Bahman, Ansari, Aseem Z., Brow, David A.

“…Functional engagement of RNA polymerase II (Pol II) with eukaryotic chromosomes is a fundamental and highly regulated biological process. Here we present a…”
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RNA-binding protein Nrd1 directs poly(A)-independent 3′-end formation of RNA polymerase II transcripts by Brow, David A, Steinmetz, Eric J, Conrad, Nicholas K, Corden, Jeffry L

“…A eukaryotic chromosome contains many genes, each transcribed separately by RNA polymerase (pol) I, II or III. Transcription termination between genes prevents…”
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Ssu72 Protein Mediates Both Poly(A)-Coupled and Poly(A)-Independent Termination of RNA Polymerase II Transcription by Steinmetz, Eric J., Brow, David A.

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LucY: A Versatile New Fluorescent Reporter Protein by Auldridge, Michele E, Cao, Hongnan, Sen, Saurabh, Franz, Laura P, Bingman, Craig A, Yennamalli, Ragothaman M, Phillips, Jr, George N, Mead, David, Steinmetz, Eric J

“…We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus…”
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cis- and trans-Acting Determinants of Transcription Termination by Yeast RNA Polymerase II by Steinmetz, Eric J., Ng, Sarah B. H., Cloute, Joseph P., Brow, David A.

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Control of pre-mRNA accumulation by the essential yeast protein Nrd1 requires high-affinity transcript binding and a domain implicated in RNA polymerase II association by Steinmetz, E.J. (University of Wisconsin Medical School, Madison, WI.), Brow, D.A

“…Nrd1 is an essential yeast protein of unknown function that has an RNA recognition motif (RRM) in its carboxyl half and a putative RNA polymerase II-binding…”
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Screening Fusion Tags for Improved Recombinant Protein Expression in E. coli with the Expresso® Solubility and Expression Screening System by Steinmetz, Eric J, Auldridge, Michele E

“…The simplicity, speed, and low cost of bacterial culture make E. coli the system of choice for most initial trials of recombinant protein expression. However,…”
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yeast heterogeneous nuclear ribonucleoprotein complex associated with RNA polymerase II by Conrad, N.K, Wilson, S.M, Steinmetz, E.J, Patturajan, M, Brow, D.A, Swanson, M.S, Corden, J.L

“…Recent evidence suggests a role for the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (pol II) in pre-mRNA processing. The yeast…”
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Evidence Supporting a Tethered Tracking Model for Helicase Activity of Escherichia coli Rho Factor by Steinmetz, Eric J., Platt, Terry

“…Transcription termination factor Rho of Escherichia coli has an ATP-dependent RNA·DNA helicase activity that presumably facilitates RNA transcript release from…”
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Pre-mRNA Processing and the CTD of RNA Polymerase II: The Tail That Wags the Dog? by Steinmetz, Eric J.

“…Eukaryotic cells must execute a complex program to generate mature functional messenger RNA (mRNA). After the transcription of a nuclear gene by RNA polymerase…”
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Expresso® Cloning and Expression Systems streamline recombinant protein expression by Sen, Saurabh, Sternard, Heather, Franz, Laura, Drinkwater, Colleen, Sheets, Lynne, Niebauer, Chris, Mead, David, Godiska, Ron, Steinmetz, Eric J

“…Abstract only The Expresso® Cloning and Expression Systems use Expressioneering™ Technology, an in vivo recombinational cloning strategy, to dramatically…”
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Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1 by Steinmetz, Eric J., Brow, David A.

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A short intervening structure can block rho factor helicase action at a distance by Steinmetz, E J, Brennan, C A, Platt, T

“…We have characterized the helicase activity of transcription termination factor rho on a variety of substrates. Helicase activity requires specific recognition…”
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Specificity and efficiency of rho-factor helicase activity depends on magnesium concentration and energy coupling to NTP hydrolysis by BRENNAN, C. A, STEINMETZ, E. J, SPEAR, P, PLATT, T

“…The RNA-DNA helicase activity of Escherichia coli transcription termination factor rho can be significantly enhanced at lower potassium chloride and magnesium…”
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