Search Results - "Steiner, Paul"

Generative adversarial network model to classify human induced pluripotent stem cell-cardiomyocytes based on maturation level by Wu, Ziqian, Park, Jiyoon, Steiner, Paul R., Zhu, Bo, Zhang, John X. J.

“…Our study develops a generative adversarial network (GAN)-based method that generates faithful synthetic image data of human cardiomyocytes at varying stages…”
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Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds by Beltrán, Jesús, Steiner, Paul J., Bedewitz, Matthew, Wei, Shuang, Peterson, Francis C., Li, Zongbo, Hughes, Brigid E., Hartley, Zachary, Robertson, Nicholas R., Medina-Cucurella, Angélica V., Baumer, Zachary T., Leonard, Alison C., Park, Sang-Youl, Volkman, Brian F., Nusinow, Dmitri A., Zhong, Wenwan, Wheeldon, Ian, Cutler, Sean R., Whitehead, Timothy A.

“…A general method to generate biosensors for user-defined molecules could provide detection tools for a wide range of biological applications. Here, we describe…”
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A Closed Form Model for Molecular Ratchet-Type Chemically Induced Dimerization Modules by Steiner, Paul J., Swift, Samuel D., Bedewitz, Matthew, Wheeldon, Ian, Cutler, Sean R., Nusinow, Dmitri A., Whitehead, Timothy A.

“…Chemical-induced dimerization (CID) modules enable users to implement ligand-controlled cellular and biochemical functions for a number of problems in basic…”
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A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation by Strawn, Isabell K, Steiner, Paul J, Newton, Matilda S, Baumer, Zachary T, Whitehead, Timothy A

“…Construction of user-defined long circular single stranded DNA (cssDNA) and linear single stranded DNA (lssDNA) is important for various biotechnological…”
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Highly variable mRNA half‐life time within marine bacterial taxa and functional genes by Steiner, Paul A., De Corte, Daniele, Geijo, Javier, Mena, Catalina, Yokokawa, Taichi, Rattei, Thomas, Herndl, Gerhard J., Sintes, Eva

“…Summary Messenger RNA can provide valuable insights into the variability of metabolic processes of microorganisms. However, due to uncertainties that include…”
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Computational Modeling of Synthetic Microbial Biofilms by Rudge, Timothy J, Steiner, Paul J, Phillips, Andrew, Haseloff, Jim

“…Microbial biofilms are complex, self-organized communities of bacteria, which employ physiological cooperation and spatial organization to increase both their…”
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An integrated technology for quantitative wide mutational scanning of human antibody Fab libraries by Petersen, Brian M., Kirby, Monica B., Chrispens, Karson M., Irvin, Olivia M., Strawn, Isabell K., Haas, Cyrus M., Walker, Alexis M., Baumer, Zachary T., Ulmer, Sophia A., Ayala, Edgardo, Rhodes, Emily R., Guthmiller, Jenna J., Steiner, Paul J., Whitehead, Timothy A.

“…Antibodies are engineerable quantities in medicine. Learning antibody molecular recognition would enable the in silico design of high affinity binders against…”
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One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening by Francino-Urdaniz, Irene M., Steiner, Paul J., Kirby, Monica B., Zhao, Fangzhu, Haas, Cyrus M., Barman, Shawn, Rhodes, Emily R., Leonard, Alison C., Peng, Linghang, Sprenger, Kayla G., Jardine, Joseph G., Whitehead, Timothy A.

“…The potential emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) escape mutants is a threat to the efficacy of existing…”
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Stabilization of the SARS-CoV-2 receptor binding domain by protein core redesign and deep mutational scanning by Leonard, Alison C, Weinstein, Jonathan J, Steiner, Paul J, Erbse, Annette H, Fleishman, Sarel J, Whitehead, Timothy A

“…Abstract Stabilizing antigenic proteins as vaccine immunogens or diagnostic reagents is a stringent case of protein engineering and design as the exterior…”
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Impact of In Vivo Protein Folding Probability on Local Fitness Landscapes by Faber, Matthew S, Wrenbeck, Emily E, Azouz, Laura R, Steiner, Paul J, Whitehead, Timothy A

“…Abstract It is incompletely understood how biophysical properties like protein stability impact molecular evolution and epistasis. Epistasis is defined as…”
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Criticality and Adaptivity in Enzymatic Networks by Steiner, Paul J., Williams, Ruth J., Hasty, Jeff, Tsimring, Lev S.

“…The contrast between the stochasticity of biochemical networks and the regularity of cellular behavior suggests that biological networks generate robust…”
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Functional Seasonality of Free-Living and Particle-Associated Prokaryotic Communities in the Coastal Adriatic Sea by Steiner, Paul A, Geijo, Javier, Fadeev, Eduard, Obiol, Aleix, Sintes, Eva, Rattei, Thomas, Herndl, Gerhard J

“…Marine snow is an important habitat for microbes, characterized by chemical and physical properties contrasting those of the ambient water. The higher nutrient…”
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Heart-on-Chip for Combined Cellular Dynamics Measurements and Computational Modeling Towards Clinical Applications by Park, Jiyoon, Wu, Ziqian, Steiner, Paul R., Zhu, Bo, Zhang, John X. J.

“…Organ-on-chip or micro-engineered three-dimensional cellular or tissue models are increasingly implemented in the study of cardiovascular pathophysiology as…”
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HRS White Paper on interoperability of data from cardiac implantable electronic devices (CIEDs) by Slotwiner, David J., Abraham, Robert L., Al-Khatib, Sana M., Anderson, H. Vernon, Bunch, T. Jared, Ferrara, Martha G., Lippman, Neal, Serwer, Gerald A., Steiner, Paul R., Tcheng, James E., Varma, Niraj, Wilkoff, Bruce L.

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A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking Mutagenesis by Steiner, Paul J, Baumer, Zachary T, Whitehead, Timothy A

“…Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly…”
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A yeast surface display platform for plant hormone receptors: Toward directed evolution of new biosensors by Steiner, Paul J., Bedewitz, Matthew A., Medina‐Cucurella, Angélica V., Cutler, Sean R., Whitehead, Timothy A.

“…Small‐molecule biosensors have major applications in biotechnology and medicine but remain difficult to engineer. Plant hormone receptors represent an…”
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User-defined single pot mutagenesis using unamplified oligo pools by Medina-Cucurella, Angélica V, Steiner, Paul J, Faber, Matthew S, Beltrán, Jesús, Borelli, Alexandra N, Kirby, Monica B, Cutler, Sean R, Whitehead, Timothy A

“…Abstract User-defined mutagenic libraries are fundamental for applied protein engineering workflows. Here we show that unamplified oligo pools can be used to…”
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Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning by Haas, Cyrus M., Francino-Urdaniz, Irene M., Steiner, Paul J., Whitehead, Timothy A.

“…Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain…”
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Seasonal dynamics of marine snow‐associated and free‐living demethylating bacterial communities in the coastal northern Adriatic Sea by Steiner, Paul A., Sintes, Eva, Simó, Rafel, De Corte, Daniele, Pfannkuchen, Daniela Marić, Ivančić, Ingrid, Najdek, Mirjana, Herndl, Gerhard J.

“…Summary The extent of DMSP demethylation has been hypothesized to depend on DMSP availability and bacterial sulfur demand, which might lead to niche…”
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Temporal dynamics in the free-living bacterial community composition in the coastal North Sea by Eva, Sintes, Harry, Witte, Karen, Stodderegger, Paul, Steiner, Gerhard J., Herndl

“…Abstract The coastal North Sea is characterized by strong seasonal dynamics in abiotic and biotic variables. Hence, pronounced temporal changes in the…”
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