Imaging Cells and Tissues with Refractive Index Radiology

Imaging Cells and Tissues with Refractive Index Radiology

Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enh...

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Bibliographic Details
Published in:Biophysical journal Vol. 87; no. 6; pp. 4180 - 4187
Main Authors: Hwu, Y., Tsai, W.L., Chang, H.M., Yeh, H.I., Hsu, P.C., Yang, Y.C., Su, Y.T., Tsai, H.L., Chow, G.M., Ho, P.C., Li, S.C., Moser, H.O., Yang, P., Seol, S.K., Kim, C.C., Je, J.H., Stefanekova, E., Groso, A., Margaritondo, G.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-12-2004
Biophysical Society
Subjects:
Animals
Cells
Cells, Cultured - diagnostic imaging
Cellular biology
Humans
Microscopy
Radiographic Image Enhancement - methods
Radiographic Image Interpretation, Computer-Assisted - methods
Radiography - methods
Radiology
Refractometry - methods
Spectroscopy, Imaging, Other Techniques
Tissues
Online Access:Get full text
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Description
Summary:Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.
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Address reprint requests to J. H. Je, E-mail: jhje@postech.ac.kr.
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.103.034991