Search Results - "Steed, P Ryan"

Toward the Fourth Dimension of Membrane Protein Structure: Insight into Dynamics from Spin-Labeling EPR Spectroscopy by Mchaourab, Hassane S., Steed, P. Ryan, Kazmier, Kelli

“…Trapping membrane proteins in the confines of a crystal lattice obscures dynamic modes essential for interconversion between multiple conformations in the…”
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Sodium and proton coupling in the conformational cycle of a MATE antiporter from Vibrio cholerae by Claxton, Derek P., Jagessar, Kevin L., Steed, P. Ryan, Stein, Richard A., Mchaourab, Hassane S.

“…Secondary active transporters belonging to the multidrug and toxic compound extrusion (MATE) family harness the potential energy of electrochemical ion…”
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Aqueous Accessibility to the Transmembrane Regions of Subunit c of the Escherichia coli F1F0 ATP Synthase by Steed, P. Ryan, Fillingame, Robert H.

“…Rotary catalysis in F1F0 ATP synthase is powered by proton translocation through the membrane-embedded F0 sector. Proton binding and release occur in the…”
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Interacting cytoplasmic loops of subunits a and c of Escherichia coli F₁F₀ ATP synthase gate H⁺ transport to the cytoplasm by Steed, P. Ryan, Kraft, Kaitlin A., Fillingame, Robert H.

“…Significance H ⁺ transport through the membrane-traversing F ₁F ₀ ATP synthase mechanically drives bond formation between ATP and P ᵢ. For the enzyme in the…”
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Subunit a Facilitates Aqueous Access to a Membrane-embedded Region of Subunit c in Escherichia coli F1F0 ATP Synthase by Steed, P. Ryan, Fillingame, Robert H.

“…Rotary catalysis in F1F0 ATP synthase is powered by proton translocation through the membrane-embedded F0 sector. Proton binding and release occurs in the…”
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Development and Implementation of a Research-Preparative General Chemistry Laboratory Course: Chemistry of Beverages by Love, Oksana, Heying, Melanie, Steed, P. Ryan, Wasileski, Sally A.

“…In the University of North Carolina Asheville Chemistry and Biochemistry Department, we believe that research with undergraduates is the most effective…”
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Generating Publishable Data from Course-Based Undergraduate Research Experiences in Chemistry by Wolfe, Amanda L., Steed, P. Ryan

“…Embedding Course-based Undergraduate Research Experiences (CUREs) into chemistry curricula has become a best practice due to the overwhelming evidence that…”
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Half channels mediating H+ transport and the mechanism of gating in the Fo sector of Escherichia coli F1Fo ATP synthase by Fillingame, Robert H., Steed, P. Ryan

“…H+-transporting F1Fo ATP synthase catalyzes the synthesis of ATP via coupled rotary motors within Fo and F1. H+ transport at the subunit a–c interface in…”
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Mutational analysis of a conserved positive charge in the c-ring of E. coli ATP synthase by Shrestha, Rashmi K, Founds, Michael W, Shepard, Sam J, Rothrock, Mallory M, Defnet, Amy E, Steed, P Ryan

“…F F ATP synthase is a ubiquitous molecular motor that utilizes a rotary mechanism to synthesize adenosine triphosphate (ATP), the fundamental energy currency…”
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Fluidity of Structure and Swiveling of Helices in the Subunit c Ring of Escherichia coli ATP Synthase as Revealed by Cysteine-Cysteine Cross-Linking by Vincent, Owen D., Schwem, Brian E., Steed, P. Ryan, Jiang, Warren, Fillingame, Robert H.

“…Subunit c in the membrane-traversing F0 sector of Escherichia coli ATP synthase is known to fold with two transmembrane helices and form an oligomeric ring of…”
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Residues in the polar loop of subunit c in Escherichia coli ATP synthase function in gating proton transport to the cytoplasm by Steed, P Ryan, Fillingame, Robert H

“…Rotary catalysis in F1F0 ATP synthase is powered by proton translocation through the membrane-embedded F0 sector. Proton binding and release occur in the…”
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Amine Basicity of Quinoline ATP Synthase Inhibitors Drives Antibacterial Activity against Pseudomonas aeruginosa by Ward, Katie T., Williams, Alexander P. L., Blair, Courtney A., Chatterjee, Ananya M., Karthikeyan, Abirami, Roper, Addison S., Kellogg, Casey N., Steed, P. Ryan, Wolfe, Amanda L.

“…Pseudomonas aeruginosa (PA), a Gram-negative pathogen, is a common cause of nosocomial infections, especially in immunocompromised and cystic fibrosis…”
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Structure and pH-Induced Structural Rearrangements of the Putative Multidrug Efflux Pump EmrD in Liposomes Probed by Site-Directed Spin Labeling by Steed, P. Ryan, Zou, Ping, Trone, Kristin E, Mchaourab, Hassane S

“…EmrD is the only structurally characterized drug/H+ antiporter of the major facilitator superfamily (MFS). It has been crystallized in a doubly occluded…”
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Na+–Substrate Coupling in the Multidrug Antiporter NorM Probed with a Spin-Labeled Substrate by Steed, P. Ryan, Stein, Richard A, Mishra, Smriti, Goodman, Michael C, Mchaourab, Hassane S

“…NorM of the multidrug and toxic compound extrusion (MATE) family of transporters couples the efflux of a broad range of hydrophobic molecules to an inward Na+…”
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Quinoline Compounds Targeting the c -Ring of ATP Synthase Inhibit Drug-Resistant Pseudomonas aeruginosa by Fraunfelter, Vesper M, Pugh, Bryce A, Williams, Alexander P L, Ward, Katie T, Jackson, Dietrich O, Austin, Molly, Ciprich, John F, Dippy, Lorelei, Dunford, Jason, Edwards, G Nathaniel, Glass, Evan, Handy, Kyle M, Kellogg, Casey N, Llewellyn, Kaitlyn, Nyberg, K Quinn, Shepard, Sam J, Thomas, Casey, Wolfe, Amanda L, Steed, P Ryan

“…(PA) is a Gram-negative, biofilm-forming bacterium and an opportunistic pathogen. The growing drug resistance of PA is a serious threat that necessitates the…”
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Synthesis and Evaluation of Pseudomonas aeruginosa ATP Synthase Inhibitors by Ciprich, John F., Buckhalt, Alexander J. E., Carroll, Lane L., Chen, David, DeFiglia, Steven A., McConnell, Riley S., Parmar, Dhruvi J., Pistor, Olivia L., Rao, Aliyah B., Rubin, M. Lillian, Volk, Grace E., Steed, P. Ryan, Wolfe, Amanda L.

“…New antibiotics with unique biological targets are desperately needed to combat the growing number of resistant bacterial pathogens. ATP synthase, a critical…”
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Proton Transport at the Interface of Subunit a and the Rotor Ring of Escherichia coli ATP Synthase by Steed, P Ryan

“…Rotary catalysis in F1F0 ATP synthase is powered by proton translocation through the membrane-embedded F0 sector. Proton binding and release occur in the…”
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Residues in the Polar Loop of Subunit c in Escherichia coli ATP Synthase Function in Gating Proton Transport to the CytoplasmThis work was supported, in whole or in part, by National Institutes of Health Grant GM23105 from the United States Public Health Service by Steed, P. Ryan, Fillingame, Robert H.

“…Rotary catalysis in F1F0 ATP synthase is powered by proton translocation through the membrane-embedded F0 sector. Proton binding and release occur in the…”
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Interacting cytoplasmic loops of subunits a and c of Escherichia coli F 1 F 0 ATP synthase gate H + transport to the cytoplasm by Steed, P. Ryan, Kraft, Kaitlin A., Fillingame, Robert H.

“…H + transport through the membrane-traversing F 1 F 0 ATP synthase mechanically drives bond formation between ATP and P i . For the enzyme in the inner…”
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Interacting cytoplasmic loops of subunits a and c of Escherichia coli F^sub 1^F^sub 0^ ATP synthase gate H+ transport to the cytoplasm by Steed, P Ryan, Kraft, Kaitlin A, Fillingame, Robert H

“…H+-transporting ... ATP synthase catalyzes the synthesis of ATP via coupled rotary motors within F0 and F1. H+ transport at the subunit a-c interface in…”
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